scholarly journals Photodynamic action of Hypericum perforatum hydrophilic extract against Staphylococcus aureus

2020 ◽  
Vol 19 (3) ◽  
pp. 324-331
Author(s):  
Pietro Delcanale ◽  
Cormac Hally ◽  
Santi Nonell ◽  
Silvia Bonardi ◽  
Cristiano Viappiani ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Hypericum Perforatum ◽  
Photodynamic Action ◽  
Naturally Occurring ◽  
Hydrophilic Extract

Hypericin (Hyp) is one of the most effective, naturally occurring photodynamic agents, which proved effective against a wide array of microorganisms.

Survival of Bacteria in “Soul Foods” at 10-Centigrade

1981 ◽  
Vol 44 (4) ◽  
pp. 271-274
Author(s):  
ADELLE W. STEWART
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Typhimurium ◽  
Food Samples ◽  
Viable Count ◽  
Inoculum Size ◽  
Farm Families ◽  
Farm Family ◽  
Collard Greens ◽  
Naturally Occurring

The fate of naturally occurring and added bacterial pathogens was determined in “soul foods” purchased at local supermarkets and farm families while the foods were stored under conditions simulating those used for retail distribution, home storage, and preparation before use. Viable count determinations for 10 samples at the end of a 5-day period at 10 C showed considerable decreases in comparison to the inoculum size, indicating that growth was not promoted. Escherichia coli survived in all the food samples but the populations decreased by 1 to 9 log cycles/g of food. Salmonella typhimurium survived in 59% of the food samples. Except for farm family collard greens and sausage (encased), Staphylococcus aureus remained viable in all of the foods tested an d was the only survivor in cracklings (cooked) obtained from both sources. Clostridium perfringens was detected in farm family sweet peas and 23% of the pig offal samples.

scholarly journals Evaluation of Antibacterial and Anti-biofilm Activities of Cinchona Alkaloid Derivatives against Staphylococcus aureus

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Malena E. Skogman ◽  
Janni Kujala ◽  
Igor Busygin ◽  
Reko Leino ◽  
Pia M. Vuorela ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Cinchona Alkaloids ◽  
Cinchona Alkaloid ◽  
Bacterial Biofilms ◽  
Planktonic Bacteria ◽  
Naturally Occurring ◽  
Resazurin Assay ◽  
Synthetic Derivative ◽  
Synthetic Derivatives

Bacterial biofilms are resistant to most of the commonly available antibacterial chemotherapies. Thus, an enormous need exists to meet the demands of effective anti-biofilm therapy. In this study, a small library of cinchona alkaloids, including the naturally occurring compounds cinchonidine and cinchonine, as well as various synthetic derivatives and analogues was screened for antibacterial and anti-biofilm activity against the Staphylococcus aureus biofilm producing strain ATCC 25923. Two methods were used to evaluate activity against biofilms, namely crystal violet staining to measure biomass and resazurin assay to measure biofilms viability. Cinchonidine was found to be inactive, whereas a synthetic derivative, 11-triphenylsilyl-10,11-dihydrocinchonidine (11-TPSCD), was effective against planktonic bacteria as well as in preventing biofilm formation at low micromolar concentrations. Higher concentrations were required to eradicate mature biofilms.

scholarly journals Experimental Ovine Mastitis; A Pathologic Study

1970 ◽  
Vol 7 (3) ◽  
pp. 246-264 ◽  
Author(s):  
M.F. El Etreby ◽  
Y.M. Abdel-Hamid
Keyword(s):  
Staphylococcus Aureus ◽  
Mammary Glands ◽  
The Other ◽  
Pathologic Study ◽  
Naturally Occurring

Strains of Staphylococcus aureus, Corynebacterium pyogenes, and Corynebacterium ovis isolated from naturally occurring, cases of ovine mastitis in Egypt were inoculated unilaterally via the teats into the mammary glands of 20 native-bred ewes, and the course and pathogenesis of the resulting changes were described. One ewe of the group infected with S. aureus developed mastitis acuta gravis. The other ewes of this group had Galactophoritis et Mastitis catarrbalis chronica et acuta. The ewes infected with G. pyogenes or with mixed G. pyogenes and S. aureus developed Galactophoritis et Mastitis catarrbatis purulenta acuta et chronica. The ewes infected with C. ovis had Mastitis apostematosa chronica. The mastitis was unilateral in all animals.

scholarly journals Non-spa-Typeable Clinical Staphylococcus aureus Strains Are Naturally Occurring Protein A Mutants

2009 ◽  
Vol 47 (11) ◽  
pp. 3624-3629 ◽  
Author(s):  
C. Baum ◽  
B. Haslinger-Loffler ◽  
H. Westh ◽  
K. Boye ◽  
G. Peters ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Naturally Occurring

Antimicrobial activity of plumbagin, a naturally occurring naphthoquinone from Plumbago rosea, against Staphylococcus aureus and Candida albicans

2016 ◽  
Vol 306 (4) ◽  
pp. 237-248 ◽  
Author(s):  
Sweatha V. Nair ◽  
Gaurav Baranwal ◽  
Maitrayee Chatterjee ◽  
Arun Sachu ◽  
Anil Kumar Vasudevan ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Naturally Occurring ◽  
Plumbago Rosea

scholarly journals Case report: characterization of a persistent, treatment-resistant, novel Staphylococcus aureus infection causing chronic mastitis in a Holstein dairy cow

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Ellie J. Putz ◽  
Mitchell V. Palmer ◽  
Hao Ma ◽  
Eduardo Casas ◽  
Timothy A. Reinhardt ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Dairy Cow ◽  
Immune Escape ◽  
Collecting Duct ◽  
Clinical Signs ◽  
Treatment Resistance ◽  
Health Concern ◽  
Valuable Insight ◽  
Naturally Occurring

Abstract Background Mastitis is the most common health concern plaguing the modern dairy cow and costs dairy producers estimates of two billion dollars annually. Staphylococcus aureus infections are prevalent, displaying varied disease presentation and markedly low cure rates. Neutrophils are considered the first line of defense against mastitis causing bacteria and are frequently targeted in the development of treatment and prevention technologies. We describe a case of naturally occurring, chronic mastitis in a Holstein cow (1428), caused by a novel strain of S. aureus that was not able to be cleared by antibiotic treatment. Case presentation The infection was identified in a single quarter, 2 months into the cow’s first lactation. The infection persisted for the following 20 months, including through dry off, and a second calving and lactation. This case of mastitis was associated with a consistently high somatic cell count, however presented with no other clinical signs. This cow was unsuccessfully treated with antibiotics commonly used to treat mastitis, consisting of two rounds of treatment during lactation and an additional round at the beginning of dry off. The chronic infection was also unchanged through an experimental mid-lactation treatment with pegylated granulocyte-colony stimulating factor (PEG-gCSF) and an additional periparturient treatment with PEG-gCSF. We isolated milk neutrophils from 1428 and compared them to two cows challenged with experimental S. aureus, strain Newbould 305. Neutrophils from 1428’s milk had higher surface expression of myeloperoxidase compared to experimental Newbould challenged animals, as well as increased presence of Neutrophil Extracellular Traps. This suggests a heightened activation state of neutrophils sourced from 1428’s naturally occurring infection. Upon postmortem examination, the affected quarter revealed multifocal abscesses separated by fibrous connective tissues. Abscesses were most common in the gland cistern and collecting duct region. Microscopically, the inflammatory reaction was pyogranulomatous to granulomatous and consistent with botryomycosis. Colonies of Gram-positive cocci were found within the eosinophilic matrix of the Splendore-Hoeppli reaction within granulomas and intracellularly within the acinar epithelium. Conclusions Collectively, we describe a unique case of chronic mastitis, the characterization of which provides valuable insight into the mechanics of S. aureus treatment resistance and immune escape.

scholarly journals Evaluation of Durability to Laundering of Triclosan and Chitosan on a Textile Substrate

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Anupama Sargur Ranganath ◽  
Ajoy K. Sarkar
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Agents ◽  
Test Methods ◽  
Hospital Environment ◽  
Cotton Textile ◽  
Current Investigation ◽  
Effective Measure ◽  
Naturally Occurring ◽  
Transmission Of Disease

In a hospital environment, textile substrates have been implicated as a vector in the transmission of disease. To mitigate the harmful spread of disease via textile substrates, an effective measure is treatment of textiles with antimicrobial agents. The current investigation compares one of the most widely used chemically synthesized antimicrobial agents, triclosan with chitosan, a naturally occurring antimicrobial agent. For the study, samples of a common polyester/cotton textile used in hospital settings were treated with triclosan and chitosan based antimicrobial agents. Following treatment, the samples were analyzed for their effectiveness against Staphylococcus aureus and Escherichia coli using standard AATCC Test Methods. The efficacy of the treatment to laundering was then evaluated by subjecting the treated samples to 50 washings and repeating the tests against the challenge microorganisms. Data obtained were statistically analyzed at a 95% confidence interval. Results showed that before laundering both triclosan and chitosan treated samples were extremely effective as antimicrobial agents. After laundering, chitosan was less effective against E.coli but maintained efficacy against S. aureus. The effectiveness of triclosan was not adversely affected after the laundering treatments.

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