Leak-free million-fold DNA amplification with locked nucleic acid and targeted hybridization in one pot

2019 ◽  
Vol 17 (23) ◽  
pp. 5708-5713 ◽  
Author(s):  
K. Komiya ◽  
M. Komori ◽  
C. Noda ◽  
S. Kobayashi ◽  
T. Yoshimura ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Amplification ◽  
Locked Nucleic Acid ◽  
One Pot ◽  
Single Stranded Dna ◽  
Free Dna ◽  
Amplification Reaction

The leak-free DNA amplification reaction supplies a large quantity of single-stranded DNA for directing molecular nanodevices in response to nucleic acid stimuli.

Conformationally Locked Aryl C-Nucleosides: Synthesis of Phosphoramidite Monomers and Incorporation into Single-Stranded DNA and LNA (Locked Nucleic Acid).

ChemInform ◽  
2003 ◽  
Vol 34 (15) ◽  
Author(s):  
B. Ravindra Babu ◽  
Ashok K. Prasad ◽  
Smriti Trikha ◽  
Niels Thorup ◽  
Virinder S. Parmar ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Single Stranded Dna ◽  
Conformationally Locked

Direct detection of circulating free DNA extracted from serum samples of breast cancer using locked nucleic acid molecular beacon

Talanta ◽  
2016 ◽  
Vol 154 ◽  
pp. 520-525 ◽  
Author(s):  
Zhen Gui ◽  
Quanbo Wang ◽  
Jinchang Li ◽  
Mingchen Zhu ◽  
Lili Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nucleic Acid ◽  
Molecular Beacon ◽  
Direct Detection ◽  
Locked Nucleic Acid ◽  
Serum Samples ◽  
Circulating Free Dna ◽  
Free Dna

scholarly journals Locked Nucleic Acid Pentamers as Universal PCR Primers for Genomic DNA Amplification

PLoS ONE ◽  
2008 ◽  
Vol 3 (11) ◽  
pp. e3701 ◽  
Author(s):  
Zhen Sun ◽  
Zhi Chen ◽  
Xiaoli Hou ◽  
Shuping Li ◽  
Haihong Zhu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Genomic Dna ◽  
Dna Amplification ◽  
Pcr Primers ◽  
Locked Nucleic Acid

scholarly journals A mini DNA-RNA hybrid origami nanobrick

2021 ◽  
Author(s):  
Lifeng Zhou ◽  
Arun Richard Chandrasekaran ◽  
Mengwen Yan ◽  
Vibhav A. Valsangkar ◽  
Jeremy I. Feldblyum ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Dna Origami ◽  
Complex Geometries ◽  
Single Stranded Dna ◽  
Dna Scaffold

DNA origami is typically used to fold a long single-stranded DNA scaffold into nanostructures with complex geometries using many short DNA staple strands. Integration of RNA into nucleic acid nanostructures...

Trinucleotide Cap Analogue Bearing a Locked Nucleic Acid Moiety: Synthesis, mRNA Modification, and Translation for Therapeutic Applications

2021 ◽  
Author(s):  
Annamalai Senthilvelan ◽  
Tyson Vonderfecht ◽  
Muthian Shanmugasundaram ◽  
Indra Pal ◽  
Jason Potter ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Acid Moiety ◽  
Therapeutic Applications

scholarly journals Nitrocellulose-bound achromopeptidase for point-of-care nucleic acid tests

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Georgios Chondrogiannis ◽  
Shirin Khaliliazar ◽  
Anna Toldrà ◽  
Pedro Réu ◽  
Mahiar M. Hamedi
Keyword(s):  
Nucleic Acid ◽  
Sample Preparation ◽  
Point Of Care ◽  
Dna Amplification ◽  
Recombinase Polymerase Amplification ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Enzymatic Function ◽  
Modern Biotechnology ◽  
Nucleic Acid Tests

AbstractEnzymes are the cornerstone of modern biotechnology. Achromopeptidase (ACP) is a well-known enzyme that hydrolyzes a number of proteins, notably proteins on the surface of Gram-positive bacteria. It is therefore used for sample preparation in nucleic acid tests. However, ACP inhibits DNA amplification which makes its integration difficult. Heat is commonly used to inactivate ACP, but it can be challenging to integrate heating into point-of-care devices. Here, we use recombinase polymerase amplification (RPA) together with ACP, and show that when ACP is immobilized on nitrocellulose paper, it retains its enzymatic function and can easily and rapidly be activated using agitation. The nitrocellulose-bound ACP does, however, not leak into the solution, preventing the need for deactivation through heat or by other means. Nitrocellulose-bound ACP thus opens new possibilities for paper-based Point-of-Care (POC) devices.

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