Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry

Nanoscale ◽  
2020 ◽  
Vol 12 (15) ◽  
pp. 8303-8313 ◽  
Author(s):  
Zdeněk Farka ◽  
Matthias J. Mickert ◽  
Zuzana Mikušová ◽  
Antonín Hlaváček ◽  
Pavla Bouchalová ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Tumor Markers ◽  
Upconversion Nanoparticles ◽  
High Contrast ◽  
Specific Detection ◽  
Surface Design ◽  
Background Ratio

Streptavidin-PEG-neridronate-UCNP nanoconjugates enable the specific detection of tumor markers on the surface of cancer cells with outstanding signal-to-background ratio.

Aptamer-modified sensitive nanobiosensors for the specific detection of antibiotics

2020 ◽  
Vol 8 (37) ◽  
pp. 8607-8613
Author(s):  
Ying Zhang ◽  
Bo Duan ◽  
Qing Bao ◽  
Tao Yang ◽  
Tiancheng Wei ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Energy Transfer ◽  
Fluorescence Resonance Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Upconversion Nanoparticles ◽  
Core Shell ◽  
Specific Detection ◽  
Energy Donor ◽  
Energy Acceptor

A highly selective, fluorescence resonance energy transfer (FRET) based aptasensor for enrofloxacin (ENR) detection was developed using core–shell upconversion nanoparticles as an energy donor and graphene oxide as an energy acceptor.

Cytotoxicity, genotoxicity and uptake detection of folic acid-functionalized green upconversion nanoparticles Y2O3/Er3+, Yb3+ as biolabels for cancer cells

2018 ◽  
Vol 53 (9) ◽  
pp. 6665-6680 ◽  
Author(s):  
D. Chávez-García ◽  
K. Juarez-Moreno ◽  
C. H. Campos ◽  
E. M. Tejeda ◽  
J. B. Alderete ◽  
...  
Keyword(s):  
Folic Acid ◽  
Cancer Cells ◽  
Upconversion Nanoparticles

scholarly journals Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 820 ◽  
Author(s):  
Dmitry Polikarpov ◽  
Liuen Liang ◽  
Andrew Care ◽  
Anwar Sunna ◽  
Douglas Campbell ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
Cancer Cells ◽  
Near Infrared ◽  
Carcinoma Cells ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Silica Layer ◽  
Bladder Cancer Cells

Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.

scholarly journals Evaluation of a Panel of Tumor Markers for Molecular Detection of Circulating Cancer Cells in Women with Suspected Breast Cancer

2005 ◽  
Vol 11 (10) ◽  
pp. 3722-3732 ◽  
Author(s):  
Monica M. Reinholz ◽  
Andrea Nibbe ◽  
Leslie M. Jonart ◽  
Kathleen Kitzmann ◽  
Vera J. Suman ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Tumor Markers ◽  
Molecular Detection ◽  
Circulating Cancer Cells

scholarly journals Single-band upconversion nanoprobes for multiplexed simultaneous in situ molecular mapping of cancer biomarkers

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Lei Zhou ◽  
Rui Wang ◽  
Chi Yao ◽  
Xiaomin Li ◽  
Chengli Wang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Western Blotting ◽  
Breast Cancer Cells ◽  
Molecular Mapping ◽  
Single Band ◽  
Upconversion Nanoparticles ◽  
Single Colour ◽  
Tissue Specimens

Abstract The identification of potential diagnostic markers and target molecules among the plethora of tumour oncoproteins for cancer diagnosis requires facile technology that is capable of quantitatively analysing multiple biomarkers in tumour cells and tissues. Diagnostic and prognostic classifications of human tumours are currently based on the western blotting and single-colour immunohistochemical methods that are not suitable for multiplexed detection. Herein, we report a general and novel method to prepare single-band upconversion nanoparticles with different colours. The expression levels of three biomarkers in breast cancer cells were determined using single-band upconversion nanoparticles, western blotting and immunohistochemical technologies with excellent correlation. Significantly, the application of antibody-conjugated single-band upconversion nanoparticle molecular profiling technology can achieve the multiplexed simultaneous in situ biodetection of biomarkers in breast cancer cells and tissue specimens and produce more accurate results for the simultaneous quantification of proteins present at low levels compared with classical immunohistochemical technology.

Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

2017 ◽  
Vol 53 (87) ◽  
pp. 11889-11892 ◽  
Author(s):  
Jinlu Tang ◽  
Xiaoxiao He ◽  
Yanli Lei ◽  
Hui Shi ◽  
Qiuping Guo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cancer Cells ◽  
Sensitive Detection ◽  
Label Free ◽  
Specific Detection ◽  
Chain Reaction ◽  
Temperature Responsive ◽  
Polymerase Chain

A label-free and general thermo-controlled split apta-PCR strategy was developed and used for the sensitive and specific detection of cancer cells.

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