Temperature-responsive split aptamers coupled with polymerase chain reaction for label-free and sensitive detection of cancer cells

2017 ◽  
Vol 53 (87) ◽  
pp. 11889-11892 ◽  
Author(s):  
Jinlu Tang ◽  
Xiaoxiao He ◽  
Yanli Lei ◽  
Hui Shi ◽  
Qiuping Guo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cancer Cells ◽  
Sensitive Detection ◽  
Label Free ◽  
Specific Detection ◽  
Chain Reaction ◽  
Temperature Responsive ◽  
Polymerase Chain

A label-free and general thermo-controlled split apta-PCR strategy was developed and used for the sensitive and specific detection of cancer cells.

Polymerase chain reaction-based assay for specific detection of Rhizoctonia solani AG-3 isolates

1999 ◽  
Vol 103 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Salim Bounou ◽  
Suha H. Jabaji-Hare ◽  
Richard Hogue ◽  
Pierre M. Charest
Keyword(s):  
Polymerase Chain Reaction ◽  
Rhizoctonia Solani ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Multiplexed microRNA expression profiling by combined asymmetric PCR and label-free detection using silicon photonic sensor arrays

2018 ◽  
Vol 10 (14) ◽  
pp. 1618-1623 ◽  
Author(s):  
Richard M. Graybill ◽  
Maria C. Cardenosa-Rubio ◽  
Hongwei Yang ◽  
Mark D. Johnson ◽  
Ryan C. Bailey
Keyword(s):  
Polymerase Chain Reaction ◽  
Expression Profiling ◽  
Sensor Arrays ◽  
Label Free ◽  
Asymmetric Pcr ◽  
Chain Reaction ◽  
Photonic Sensor ◽  
Silicon Photonic ◽  
Label Free Detection ◽  
Polymerase Chain

Analysis methods based upon the quantitative, real-time polymerase chain reaction are extremely powerful; however, they face intrinsic limitations in terms of target multiplexing.

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