Enhancing the stability of DNA origami nanostructures: staple strand redesign versus enzymatic ligation

Nanoscale ◽  
2019 ◽  
Vol 11 (35) ◽  
pp. 16270-16276 ◽  
Author(s):  
Saminathan Ramakrishnan ◽  
Leonard Schärfen ◽  
Kristin Hunold ◽  
Sebastian Fricke ◽  
Guido Grundmeier ◽  
...  
Keyword(s):  
Dna Origami ◽  
Moderate Increase ◽  
The Stability ◽  
Enzymatic Ligation

Merging of bridging staples with adjacent oligonucleotide sequences leads to a moderate increase of DNA origami stability, while enzymatic ligation after assembly yields a reinforced nanostructure with superior stability at up to 37 °C and in the presence of 6 M urea.

scholarly journals Stability study of tubular DNA origami in the presence of protein crystallisation buffer

RSC Advances ◽  
2015 ◽  
Vol 5 (72) ◽  
pp. 58734-58737 ◽  
Author(s):  
Dianming Wang ◽  
Ziran Da ◽  
Bohan Zhang ◽  
Mark Antonin Isbell ◽  
Yuanchen Dong ◽  
...  
Keyword(s):  
Stability Study ◽  
Dna Origami ◽  
The Stability

A method for screening compatible buffer conditions for both DNA origami and protein crystallisation and studied how protein crystallisation buffer conditions notably cations, buffering agents, precipitants, and pH, influenced the stability of tubular DNA origami.

On the Stability of DNA Origami Nanostructures in Low-Magnesium Buffers

2018 ◽  
Vol 130 (30) ◽  
pp. 9614-9618 ◽  
Author(s):  
Charlotte Kielar ◽  
Yang Xin ◽  
Boxuan Shen ◽  
Mauri A. Kostiainen ◽  
Guido Grundmeier ◽  
...  
Keyword(s):  
Dna Origami ◽  
Low Magnesium ◽  
The Stability

The effect of crossovers on the stability of DNA origami type nanocarriers

2020 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Maryam Mogheiseh ◽  
Reza Hasanzadeh Ghasemi ◽  
Reza Soheilifard
Keyword(s):  
Medical Engineering ◽  
Dna Origami ◽  
Structure Stability ◽  
Content Type ◽  
Chemical Structures ◽  
Human Body Temperature ◽  
Different Dimensions ◽  
The Stability ◽  
Dynamics Simulations ◽  
Significant Factors

PurposeThe purpose of this paper is to compare the stability of the three nanocarriers created by DNA origami method with different positions and numbers of crossoversDesign/methodology/approachNanocarriers are attractive components among a variety of nanostructures created by DNA origami and can have numerous applications in mechanical and medical engineering. For this reason, the current study compares three nanotubes with different positions and numbers of crossovers created by DNA origami method that can be utilized as nanocarriers. To investigate the structures, the DNA nanocarriers are studied at the human body temperature 310 K. Molecular dynamics simulations are used for this study. For a quantitative analysis of DNA nanocarriers, the areas of three hexagons at three different sites in each of the nanotubes are investigated. The results indicate that the number and position of crossovers are among the significant factors in the structure stability of nanocarriers. The analyses also revealed that although adding crossovers in locations with fewer crossovers increase structural stability, the position of crossovers can have different effects on the stability. DNA origami-based nanocarriers can be implemented in drug delivery, allow the nanocargoes to pass various surfaces and act as filters for passing cargoes of different dimensions and chemical structures.FindingsThe results indicate that the number and position of crossovers are among the significant factors in the structure stability of nanocarriersOriginality/valueIn this paper, the stability of DNA origami nanocarriers with different positions and numbers of crossovers was investigated.

On the Stability of DNA Origami Nanostructures in Low-Magnesium Buffers

2018 ◽  
Vol 57 (30) ◽  
pp. 9470-9474 ◽  
Author(s):  
Charlotte Kielar ◽  
Yang Xin ◽  
Boxuan Shen ◽  
Mauri A. Kostiainen ◽  
Guido Grundmeier ◽  
...  
Keyword(s):  
Dna Origami ◽  
Low Magnesium ◽  
The Stability

scholarly journals DNA origami protection and molecular interfacing through engineered sequence-defined peptoids

2020 ◽  
Vol 117 (12) ◽  
pp. 6339-6348 ◽  
Author(s):  
Shih-Ting Wang ◽  
Melissa A. Gray ◽  
Sunting Xuan ◽  
Yiyang Lin ◽  
James Byrnes ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Electrostatic Interactions ◽  
Biological Fluids ◽  
Structural Characteristics ◽  
Dna Nanotechnology ◽  
Dna Origami ◽  
Cell Targeting ◽  
The Stability ◽  
Molecular Plasticity

DNA nanotechnology has established approaches for designing programmable and precisely controlled nanoscale architectures through specific Watson−Crick base-pairing, molecular plasticity, and intermolecular connectivity. In particular, superior control over DNA origami structures could be beneficial for biomedical applications, including biosensing, in vivo imaging, and drug and gene delivery. However, protecting DNA origami structures in complex biological fluids while preserving their structural characteristics remains a major challenge for enabling these applications. Here, we developed a class of structurally well-defined peptoids to protect DNA origamis in ionic and bioactive conditions and systematically explored the effects of peptoid architecture and sequence dependency on DNA origami stability. The applicability of this approach for drug delivery, bioimaging, and cell targeting was also demonstrated. A series of peptoids (PE1–9) with two types of architectures, termed as “brush” and “block,” were built from positively charged monomers and neutral oligo-ethyleneoxy monomers, where certain designs were found to greatly enhance the stability of DNA origami. Through experimental and molecular dynamics studies, we demonstrated the role of sequence-dependent electrostatic interactions of peptoids with the DNA backbone. We showed that octahedral DNA origamis coated with peptoid (PE2) can be used as carriers for anticancer drug and protein, where the peptoid modulated the rate of drug release and prolonged protein stability against proteolytic hydrolysis. Finally, we synthesized two alkyne-modified peptoids (PE8 and PE9), conjugated with fluorophore and antibody, to make stable DNA origamis with imaging and cell-targeting capabilities. Our results demonstrate an approach toward functional and physiologically stable DNA origami for biomedical applications.

scholarly journals European Integration and the Spatial Dynamics of Manufacturing-Employment Change

10.1068/a3543 ◽  
2002 ◽  
Vol 34 (11) ◽  
pp. 2017-2036 ◽  
Author(s):  
Konstantinos A Melachroinos
Keyword(s):  
European Integration ◽  
Spatial Dynamics ◽  
Political Rhetoric ◽  
Moderate Increase ◽  
Integration Process ◽  
Employment Change ◽  
Manufacturing Employment ◽  
Industrial Structures ◽  
European Union Countries ◽  
The Stability

The author examines the dynamics of manufacturing-employment change in thirteen European Union countries between 1978 and 1996, mainly through the utilisation of shift-share techniques. Despite the momentum that European integration gained over this period, the key finding is that the geography of manufacturing employment has remained almost intact. Processes operating at the European scale appear to have the largest impact on labour outcomes in each member state, while there is little deviation from the widespread declining trends. Spatial stability is reinforced even further through the homogeneity of several national industrial structures. In addition, the moderate increase in the industrial specialisation of the participating economies, and the localisation tendencies of a few traditional subsectors, pose little if any threat to the stability of the present map of manufacturing distribution. This evidence suggests that, contrary to some political rhetoric, the integration process has not, so far at least, adversely affected the relative competitiveness of manufacturing either in peripheral or in core countries.

Open discussion

1982 ◽  
Vol 99 ◽  
pp. 605-613
Author(s):  
P. S. Conti
Keyword(s):  
Buenos Aires ◽  
Large Mass ◽  
List Type ◽  
Common Phenomenon ◽  
Open Discussion ◽  
The Stability ◽  
Ring Nebulae

Conti: One of the main conclusions of the Wolf-Rayet symposium in Buenos Aires was that Wolf-Rayet stars are evolutionary products of massive objects. Some questions:–Do hot helium-rich stars, that are not Wolf-Rayet stars, exist?–What about the stability of helium rich stars of large mass? We know a helium rich star of ∼40 MO. Has the stability something to do with the wind?–Ring nebulae and bubbles : this seems to be a much more common phenomenon than we thought of some years age.–What is the origin of the subtypes? This is important to find a possible matching of scenarios to subtypes.

Symmetric Multistep Methods Revisited: II. Numerical Experiments

1999 ◽  
Vol 173 ◽  
pp. 309-314 ◽  
Author(s):  
T. Fukushima
Keyword(s):  
Multistep Methods ◽  
Computational Time ◽  
Integration Time ◽  
Implicit Methods ◽  
Symmetric Methods ◽  
Celestial Bodies ◽  
The Stability ◽  
Predictor Corrector ◽  
Symmetric Multistep Methods ◽  
Integration Errors

AbstractBy using the stability condition and general formulas developed by Fukushima (1998 = Paper I) we discovered that, just as in the case of the explicit symmetric multistep methods (Quinlan and Tremaine, 1990), when integrating orbital motions of celestial bodies, the implicit symmetric multistep methods used in the predictor-corrector manner lead to integration errors in position which grow linearly with the integration time if the stepsizes adopted are sufficiently small and if the number of corrections is sufficiently large, say two or three. We confirmed also that the symmetric methods (explicit or implicit) would produce the stepsize-dependent instabilities/resonances, which was discovered by A. Toomre in 1991 and confirmed by G.D. Quinlan for some high order explicit methods. Although the implicit methods require twice or more computational time for the same stepsize than the explicit symmetric ones do, they seem to be preferable since they reduce these undesirable features significantly.

Uniformity of Magnification from Different Electron Microscopes

1967 ◽  
Vol 25 ◽  
pp. 32-33
Author(s):  
Godfrey C. Hoskins ◽  
V. Williams ◽  
V. Allison
Keyword(s):  
Diffraction Grating ◽  
The Other ◽  
Carbon Replica ◽  
Electron Microscopes ◽  
The Stability

The method demonstrated is an adaptation of a proven procedure for accurately determining the magnification of light photomicrographs. Because of the stability of modern electrical lenses, the method is shown to be directly applicable for providing precise reproducibility of magnification in various models of electron microscopes.A readily recognizable area of a carbon replica of a crossed-line diffraction grating is used as a standard. The same area of the standard was photographed in Phillips EM 200, Hitachi HU-11B2, and RCA EMU 3F electron microscopes at taps representative of the range of magnification of each. Negatives from one microscope were selected as guides and printed at convenient magnifications; then negatives from each of the other microscopes were projected to register with these prints. By deferring measurement to the print rather than comparing negatives, correspondence of magnification of the specimen in the three microscopes could be brought to within 2%.

Effect of Improved ThO2 Particle Dispersion in Nickel on High-Temperature Strength

1970 ◽  
Vol 28 ◽  
pp. 444-445
Author(s):  
E. R. Kimmel ◽  
H. L. Anthony ◽  
W. Scheithauer
Keyword(s):  
High Temperature ◽  
Metal Matrix ◽  
Oxide Particle ◽  
Oxide Phase ◽  
Dislocation Motion ◽  
Particle Dispersion ◽  
High Temperature Strength ◽  
Strengthening Effect ◽  
Oxide Particles ◽  
The Stability

The strengthening effect at high temperature produced by a dispersed oxide phase in a metal matrix is seemingly dependent on at least two major contributors: oxide particle size and spatial distribution, and stability of the worked microstructure. These two are strongly interrelated. The stability of the microstructure is produced by polygonization of the worked structure forming low angle cell boundaries which become anchored by the dispersed oxide particles. The effect of the particles on strength is therefore twofold, in that they stabilize the worked microstructure and also hinder dislocation motion during loading.

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