Label-free colorimetric detection of deoxyribonuclease I activity based on the DNA-enhanced peroxidase-like activity of MIL-53(Fe)

2019 ◽  
Vol 43 (32) ◽  
pp. 12776-12784 ◽  
Author(s):  
Chan Song ◽  
Wei Ding ◽  
Haibo Liu ◽  
Weiwen Zhao ◽  
Yuewei Yao ◽  
...  

In this study, the effect of single-stranded DNA (ssDNA) on the intrinsic peroxidase-like activity of MIL-53(Fe) was investigated.

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ehsan Shokri ◽  
Morteza Hosseini ◽  
Mehdi D. Davari ◽  
Mohammad R. Ganjali ◽  
Maikel P. Peppelenbosch ◽  
...  

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Stefan D. Krämer ◽  
Johannes Wöhrle ◽  
Philipp A. Meyer ◽  
Gerald A. Urban ◽  
Günter Roth

Abstract Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.


The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4719-4724 ◽  
Author(s):  
Yaqing Chang ◽  
Zhe Zhang ◽  
Huiqing Liu ◽  
Nan Wang ◽  
Jilin Tang

In this study, we investigated the interaction of cobalt oxyhydroxide (CoOOH) nanoflakes with DNA and their fluorescence quenching mechanism of a FAM-labeled single-stranded DNA (ssDNA) probe.


2018 ◽  
Vol 10 (6) ◽  
pp. 641-645 ◽  
Author(s):  
Xu Zhu ◽  
Ningning Zhang ◽  
Yintang Zhang ◽  
Baoxia Liu ◽  
Zhu Chang ◽  
...  

A gold nanoparticle-based label-free homogeneous phase colorimetric bioassay was developed for the detection of Aβ1–40 oligomers.


Nanoscale ◽  
2017 ◽  
Vol 9 (13) ◽  
pp. 4508-4515 ◽  
Author(s):  
Na Lu ◽  
Min Zhang ◽  
Lei Ding ◽  
Jing Zheng ◽  
Caixia Zeng ◽  
...  

2017 ◽  
Vol 33 (10) ◽  
pp. 1115-1121 ◽  
Author(s):  
Elumalai SATHEESHKUMAR ◽  
Jyisy YANG ◽  
Venkatesan SRINIVASADESIKAN ◽  
Ming-Chang LIN

Sign in / Sign up

Export Citation Format

Share Document