Enhancement and control of neuron adhesion on polydimethylsiloxane for cell microengineering using a functionalized triblock polymer

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3162-3167
Author(s):  
Wenming Liu ◽  
Kai Han ◽  
Meilin Sun ◽  
Jinyi Wang
Keyword(s):  
Triblock Polymer ◽  
One Step ◽  
And Control

We present a straightforward strategy to promote and engineer neuron adhesion on a polydimethylsiloxane substrate by a one-step modification using a functionalized triblock polymer.

scholarly journals Non-Conventional Features of Plant Oil-Based Acrylic Monomers in Emulsion Polymerization

Molecules ◽  
2020 ◽  
Vol 25 (13) ◽  
pp. 2990 ◽  
Author(s):  
Ananiy Kohut ◽  
Stanislav Voronov ◽  
Zoriana Demchuk ◽  
Vasylyna Kirianchuk ◽  
Kyle Kingsley ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Physical And Mechanical Properties ◽  
Polymer Coatings ◽  
Van Der Waals Interactions ◽  
Vinyl Monomers ◽  
Plant Oil ◽  
Crosslinking Degree ◽  
One Step ◽  
And Control ◽  
Plant Sources

In recent years, polymer chemistry has experienced an intensive development of a new field regarding the synthesis of aliphatic and aromatic biobased monomers obtained from renewable plant sources. A one-step process for the synthesis of new vinyl monomers by the reaction of direct transesterification of plant oil triglycerides with N-(hydroxyethyl)acrylamide has been recently invented to yield plant oil-based monomers (POBMs). The features of the POBM chemical structure, containing both a polar (hydrophilic) fragment capable of electrostatic interactions, and hydrophobic acyl fatty acid moieties (C15-C17) capable of van der Waals interactions, ensures the participation of the POBMs fragments of polymers in intermolecular interactions before and during polymerization. The use of the POBMs with different unsaturations in copolymerization reactions with conventional vinyl monomers allows for obtaining copolymers with enhanced hydrophobicity, provides a mechanism of internal plasticization and control of crosslinking degree. Synthesized latexes and latex polymers are promising candidates for the formation of hydrophobic polymer coatings with controlled physical and mechanical properties through the targeted control of the content of different POBM units with different degrees of unsaturation in the latex polymers.

Transition-metal-catalyzed multicomponent coupling reactions with imines and carbon monoxide

2013 ◽  
Vol 85 (2) ◽  
pp. 377-384 ◽  
Author(s):  
Jeffrey S. Quesnel ◽  
Bruce A. Arndtsen
Keyword(s):  
Transition Metal ◽  
Building Blocks ◽  
Coupling Reactions ◽  
Metal Catalysis ◽  
Heterocyclic Derivatives ◽  
One Step ◽  
Multicomponent Coupling Reactions ◽  
Metal Catalyzed ◽  
And Control ◽  
Multicomponent Coupling

Multicomponent coupling reactions provide a method to combine several substrates in a single reaction. When coupled with transition-metal catalysis, these reactions can be used to both activate and control the assembly of multiple simple building blocks directly into useful products. This account will describe our work in this area, with a focus on the use of palladium catalysis as a tool to couple imines and CO with other substrates into a range of heterocyclic derivatives in one-step reactions.

Unknown Offset Free MPC for Buck Converter

2017 ◽  
Vol 865 ◽  
pp. 175-180
Author(s):  
Po Li ◽  
Rui Nan Liu ◽  
Xiang Hui Ma
Keyword(s):  
Continuous Model ◽  
Buck Converter ◽  
Power Supplies ◽  
Recursive Least Squares ◽  
Buck Converters ◽  
Dc Power ◽  
Control Scheme ◽  
Parameters Uncertainty ◽  
One Step ◽  
And Control

Buck converters are commonly used as DC power supplies. To deal with the parameters uncertainty in R-L (resistance-inductance), an Unknown Offset Free Model Predictive Control (UOFMPC) method for buck converters have been proposed in this paper. Firstly, a continuous model for buck converters is established. Based on it, a discrete model with fixed sampling time is derived and the output of controller is set as the direct switch on/off signals. Secondly, one-step MPC method aimed at optimizing the output voltage with recursive least squares algorithm for parameters identification is given to satisfy the ability of adaptation in parameters. Finally, both the model and control scheme are validated by simulation in MATLAB/Simulink.

scholarly journals Tuning Surface Morphology of Fluorescent Hydrogels Using a Vortex Fluidic Device

Molecules ◽  
2020 ◽  
Vol 25 (15) ◽  
pp. 3445
Author(s):  
Javad Tavakoli ◽  
Colin L. Raston ◽  
Youhong Tang
Keyword(s):  
Structure Property ◽  
Future Studies ◽  
Architectural Features ◽  
Structure Property Relationship ◽  
Fluidic Device ◽  
One Step ◽  
The Creation ◽  
Physically Crosslinked ◽  
And Control ◽  
Surface Morphologies

In recent decades, microfluidic techniques have been extensively used to advance hydrogel design and control the architectural features on the micro- and nanoscale. The major challenges with the microfluidic approach are clogging and limited architectural features: notably, the creation of the sphere, core-shell, and fibers. Implementation of batch production is almost impossible with the relatively lengthy time of production, which is another disadvantage. This minireview aims to introduce a new microfluidic platform, a vortex fluidic device (VFD), for one-step fabrication of hydrogels with different architectural features and properties. The application of a VFD in the fabrication of physically crosslinked hydrogels with different surface morphologies, the creation of fluorescent hydrogels with excellent photostability and fluorescence properties, and tuning of the structure–property relationship in hydrogels are discussed. We conceive, on the basis of this minireview, that future studies will provide new opportunities to develop hydrogel nanocomposites with superior properties for different biomedical and engineering applications.

scholarly journals Chikungunya (Togaviridae) and Dengue 2 (Flaviviridae) Viruses Isolated from Aedes aegypti Mosquitoes by qRT-PCR Technique: Xenosurveillance for Arboviruses Circulating in Burkina Faso

2021 ◽  
Author(s):  
Aristide Sawdetuo Hien ◽  
Ibrahim Sangaré ◽  
Eric L. Parfait Ouattara ◽  
Simon P. Sawadogo ◽  
Diloma D. Soma ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Burkina Faso ◽  
Disease Surveillance ◽  
Adult Stage ◽  
Public Health Importance ◽  
Control Programs ◽  
Qrt Pcr ◽  
One Step ◽  
Study Sites ◽  
And Control

Abstract: In 2016, we conducted an entomological survey in a railway transect between Banfora and Ouagadougou, Burkina Faso. The aim was to evaluate the risk factors for arbovirus epidemics, including vector infection status, in areas representative of the country. Aedes aegypti mosquitoes were collected at larval stage from four study sites and reared until adult stage and kept in RNAlater for detection of arbovirus RNA. In the laboratory, the mosquito specimens were screened for dengue virus (DENV) and chikungunya virus (CHIKV) using one step real-time qRT-PCR. We detected one DENV-2 positive pool from Ouagadougou, giving a minimum infec-tion rate (MIR) of 16.67, and 6 CHIKV positive pools, giving a MIR of 66.67 from Ouagadougou, but also in Banfora and Boromo. The qRT-PCR is a useful tool for the surveillance of arboviruses of public health importance in Burkina Faso and may be incorporated into disease surveillance and control programs in Burkina Faso.

scholarly journals Bioinspired electrospun dECM scaffolds guide cell growth and control the formation of myotubes

2021 ◽  
Vol 7 (20) ◽  
pp. eabg4123
Author(s):  
Mollie M. Smoak ◽  
Katie J. Hogan ◽  
K. Jane Grande-Allen ◽  
Antonios G. Mikos
Keyword(s):  
Skeletal Muscle ◽  
Physicochemical Properties ◽  
High Capacity ◽  
Skeletal Muscle Regeneration ◽  
Functional Deficits ◽  
Myotube Formation ◽  
Acute Injuries ◽  
One Step ◽  
And Control ◽  
Mouse Myoblast

While skeletal muscle has a high capacity for endogenous repair in acute injuries, volumetric muscle loss can leave long-lasting or permanent structural and functional deficits to the injured muscle and surrounding tissues. With clinical treatments failing to repair lost tissue, there is a great need for a tissue-engineered therapy to promote skeletal muscle regeneration. In this study, we aim to assess the potential for electrospun decellularized skeletal muscle extracellular matrix (dECM) with tunable physicochemical properties to control mouse myoblast growth and myotube formation. The material properties as well as cell behavior – growth and differentiation – were assessed in response to modulation of crosslinking and scaffold architecture. The fabrication of a bioactive dECM-based system with tunable physicochemical properties that can control myotube formation has several applications in skeletal muscle engineering and may bring the field one step closer to developing a therapy to address these unmet clinical needs.

Device for an Active Dimension and Deflection Control of Shafts at Machining

2006 ◽  
Vol 113 ◽  
pp. 465-470
Author(s):  
Andrejus Henrikas Marcinkevičius
Keyword(s):  
Machine Tools ◽  
Radial Direction ◽  
Main Idea ◽  
High Accuracy ◽  
Theoretical Background ◽  
Dimension Control ◽  
One Step ◽  
And Control ◽  
Neck Diameter ◽  
Direction Opposite

When using programmed machine tools many steps of a shaft are machined in one operation. Many shafts are not rigid enough and for the purpose of achieving high accuracy and productivity it is necessary to use an automatic follow rest and dimension control. The problem is complicated by necessity of an automatic reset in an operation from one step of a shaft to the other. The new scheme of a device is proposed in the paper that enables simultaneously to support the shaft being machined and control its dimensions. The work principle of a device is that the shaft is supported in a radial direction by a movable slider. Diameter of the shaft neck is measured by two levers turning round their axes driven by the movable slider with patterns. The main idea is that at motion of the slider equal to change of the shaft neck diameter measuring surfaces of levers are constantly tangent to diametrical surface of the workpiece while the slider is supporting the workpiece in radial direction, opposite to the cutting force. Dimension of a shaft neck is evaluated as a sum of motion value of a slider and deviation from tangent of lever measuring surfaces. Theoretical background of principle schemes and the work scheme of a device are presented in the paper.

scholarly journals Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection

2020 ◽  
Author(s):  
Julia Alcoba-Florez ◽  
Helena Gil-Campesino ◽  
Diego García-Martínez de Artola ◽  
Rafaela González-Montelongo ◽  
Agustín Valenzuela-Fernández ◽  
...  
Keyword(s):  
False Negative ◽  
Rna Extraction ◽  
Nasopharyngeal Swab ◽  
False Negatives ◽  
Diagnostic Screening ◽  
Extraction Step ◽  
Wide Variability ◽  
One Step ◽  
And Control ◽  
The Impact

AbstractObjectiveThe ongoing COVID-19 pandemic continues imposing a demand for diagnostic screening. In anticipation that the recurrence of outbreaks and the measures for lifting the lockdown worldwide may cause supply chain issues over the coming months, we assessed the sensitivity of a number of one-step retrotranscription and quantitative PCR (RT-qPCR) solutions to detect SARS-CoV-2.MethodsWe evaluated six different RT-qPCR alternatives for SARS-CoV-2/COVID-19 diagnosis based on standard RNA extractions. That of best sensitivity was also assessed with direct nasopharyngeal swab viral transmission medium (VTM) heating, overcoming the RNA extraction step.ResultsWe found a wide variability in the sensitivity of RT-qPCR solutions that associated with a range of false negatives from as low as 2% (0.3-7.9%) to as much as 39.8% (30.2-50.2). Direct preheating of VTM combined with the best solution provided a sensitivity of 72.5% (62.5-81.0), in the range of some of the solutions based on standard RNA extractions.ConclusionsWe evidenced sensitivity limitations of currently used RT-qPCR solutions. Our results will help to calibrate the impact of false negative diagnoses of COVID-19, and to detect and control new SARS-CoV-2 outbreaks and community transmissions.

scholarly journals Development of a Potential Penside Colorimetric LAMP Assay Using Neutral Red for Detection of African Swine Fever Virus

2021 ◽  
Vol 12 ◽  
Author(s):  
Yang Wang ◽  
Junfei Dai ◽  
Yongsheng Liu ◽  
Jifei Yang ◽  
Qian Hou ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
Animal Health ◽  
Colorimetric Detection ◽  
Monitoring Program ◽  
African Swine Fever ◽  
Lamp Assay ◽  
Neutral Red ◽  
Economic Losses ◽  
One Step ◽  
And Control

African swine fever (ASF) has caused huge economic losses to the swine industry worldwide. Since there is no commercial ASF vaccine available, an early diagnosis is extremely important to prevent and control the disease. In this study, ASF virus (ASFV) capsid protein-encoding gene (p72) was selected and used to design primers for establishing a one-step visual loop-mediated isothermal amplification (LAMP) assay with neutral red, a pH-sensitive dye, as the color shift indicator. Neutral red exhibited a sharp contrast of color change from faint orange (negative) to pink (positive) during LAMP for detection of ASFV. The designed primer set targeting highly conserved region of the p72 gene was highly specific to ASFV and showed no cross-reactivity with other swine viruses. The detection limit for the one-step visual LAMP developed was 10 copies/reaction based on the recombinant plasmid containing the p72 gene of ASFV. More importantly, the developed one-step visual LAMP showed high consistency with the results of the real-time polymerase chain reaction (qPCR) method recommended by World Organization for Animal Health (OIE). Furthermore, the results demonstrate that the colorimetric detection with this LAMP assay could be directly applied for the whole blood and serum samples without requiring genome extraction. Based on our results, the developed one-step visual LAMP assay is a promising penside diagnostic tool for development of early and cost-effective ASF monitoring program that would greatly contribute to the prevention and control of ASF.

scholarly journals Generation of highly integrated multiple vivid colours using a three-dimensional broadband perfect absorber

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Soo-Jung Kim ◽  
Pil-Hoon Jung ◽  
Wonjoong Kim ◽  
Heon Lee ◽  
Sung-Hoon Hong
Keyword(s):  
High Resolution ◽  
Three Dimensional ◽  
Perfect Absorber ◽  
Simultaneous Generation ◽  
Practical Applications ◽  
Fabry Perot ◽  
Device Applications ◽  
One Step ◽  
Colour Patterns ◽  
And Control

Abstract The colour printing technology based on interactions between geometric structures and light has various advantages over the pigment-based colour technology in terms of nontoxicity and ultrasmall pixel size. The asymmetric Fabry–Perot (F–P) cavity absorber is the simplest light-interacting structure, which can easily represent and control the colour by the thickness of the dielectric layer. However, for practical applications, an advanced manufacturing technique for the simultaneous generation of multiple reflective colours is required. In this study, we demonstrate F–P cavity absorbers with micropixels by overcoming the difficulties of multi-level pattern fabrication using a nanoimprinting approach. Our asymmetric F–P cavity absorber exhibited a high absorption (approximately 99%) in a wide visible light range upon the incorporation of lossy metallic materials, yielding vivid colours. A high-resolution image of eight different reflective colours was obtained by a one-step process. This demonstrates the potential of this technology for device applications such as high-resolution colour displays and colour patterns used for security functions.

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