Flow control for lateral flow strips with centrifugal microfluidics

Lab on a Chip ◽  
2019 ◽  
Vol 19 (16) ◽  
pp. 2718-2727 ◽  
Author(s):  
Daniel M. Kainz ◽  
Susanna M. Früh ◽  
Tobias Hutzenlaub ◽  
Roland Zengerle ◽  
Nils Paust
Keyword(s):  
Flow Control ◽  
Lateral Flow ◽  
Clinical Diagnostics ◽  
Centrifugal Microfluidics ◽  
Highly Sensitive ◽  
Lateral Flow Strips

Lateral flow strips (LFSs) are widely used for clinical diagnostics. The restricted flow control is one challenge to the development of quantitative and highly sensitive LFSs. Here, we present a flow control for LFSs using centrifugal microfluidics.

scholarly journals Paper-Based Test for Rapid On-Site Screening of SARS-CoV-2 in Clinical Samples

Biosensors ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 488
Author(s):  
Wen Ren ◽  
Joseph Irudayaraj
Keyword(s):  
Nasal Swab ◽  
Lateral Flow ◽  
Detection Methods ◽  
Clinical Samples ◽  
Virus Particles ◽  
Low Concentrations ◽  
Highly Sensitive ◽  
Lateral Flow Strips ◽  
Magnetic Probes ◽  
Colorimetric Reaction

Detection methods for monitoring infectious pathogens has never been more important given the need to contain the spread of the COVID-19 pandemic. Herein we propose a highly sensitive magnetic-focus-enhanced lateral flow assay (mLFA) for the detection of SARS-CoV-2. The proposed mLFA is simple and requires only lateral flow strips and a reusable magnet to detect very low concentrations of the virus particles. The magnetic focus enhancement is achieved by focusing the SARS-CoV-2 conjugated magnetic probes in the sample placed in the lateral flow (LF) strips for improved capture efficiency, while horseradish peroxidase (HRP) was used to catalyze the colorimetric reaction for the amplification of the colorimetric signal. With the magnetic focus enhancement and HRP-based amplification, the mLFA could yield a highly sensitive technology for the recognition of SARS-CoV-2. The developed methods could detect as low as 400 PFU/mL of SARS-CoV-2 in PBS buffer based on the visible blue dots on the LF strips. The mLFA could recognize 1200 PFU/mL of SARS-CoV-2 in saliva samples. With clinical nasal swab samples, the proposed mLFA could achieve 66.7% sensitivity and 100% specificity.

scholarly journals Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3358
Author(s):  
Donato Calabria ◽  
Maria Maddalena Calabretta ◽  
Martina Zangheri ◽  
Elisa Marchegiani ◽  
Ilaria Trozzi ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Qualitative Information ◽  
Eye Color ◽  
Commercial Success ◽  
Color Changes ◽  
Naked Eye ◽  
Future Developments ◽  
Highly Sensitive ◽  
Critical Overview ◽  
Detection Technologies

Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

scholarly journals Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 768
Author(s):  
Hyung-Mo Kim ◽  
Chiwoo Oh ◽  
Jaehyun An ◽  
Seungki Baek ◽  
Sungje Bock ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Calf Serum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Uniform Size ◽  
Highly Sensitive ◽  
Wide Range ◽  
New Biomarkers

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.

Highly sensitive multiplex lateral flow immunoassay of phytopathogens using Au@Pt nanoparticles as the colorimetric and catalytic label

2021 ◽  
Author(s):  
Vasily G. Panferov ◽  
Shyatesa C. Razo ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Pt Nanoparticles ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Highly Sensitive

Luminescent lanthanide nanoparticle-based imaging enables ultra-sensitive, quantitative and multiplexed in vitro lateral flow immunoassays

Nanoscale ◽  
2021 ◽  
Author(s):  
F. Mousseau ◽  
C. Féraudet Tarisse ◽  
S. Simon ◽  
T. Gacoin ◽  
A. Alexandrou ◽  
...  
Keyword(s):  
Optical Properties ◽  
Lateral Flow ◽  
Biomolecule Detection ◽  
Nanoparticle Probes ◽  
Highly Sensitive

We developed a portable, fast, highly sensitive and quantitative in vitro assay for on-site biomolecule detection by combining the remarkable optical properties of new lanthanide-doped nanoparticle probes with a simple reader coupled to a smartphone.

A highly sensitive dual-color lateral flow immunoassay for brucellosis using one-step synthesized latex microspheres

2019 ◽  
Vol 11 (22) ◽  
pp. 2937-2942 ◽  
Author(s):  
Mingsong Zhu ◽  
Yurui Jia ◽  
Lizhi Peng ◽  
Jifu Ma ◽  
Xiangru Li ◽  
...  
Keyword(s):  
Clinical Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Screening Methods ◽  
Step Method ◽  
Dual Color ◽  
Highly Sensitive ◽  
One Step

A lateral flow immunoassay was developed to improve clinical care compared with conventional brucellosis screening methods. Detection is dual-color in format using dyed, carboxyl-functionalized latex microspheres synthesized with a one-step method.

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