Luminescent lanthanide nanoparticle-based imaging enables ultra-sensitive, quantitative and multiplexed in vitro lateral flow immunoassays

Nanoscale ◽  
2021 ◽  
Author(s):  
F. Mousseau ◽  
C. Féraudet Tarisse ◽  
S. Simon ◽  
T. Gacoin ◽  
A. Alexandrou ◽  
...  
Keyword(s):  
Optical Properties ◽  
Lateral Flow ◽  
Biomolecule Detection ◽  
Nanoparticle Probes ◽  
Highly Sensitive

We developed a portable, fast, highly sensitive and quantitative in vitro assay for on-site biomolecule detection by combining the remarkable optical properties of new lanthanide-doped nanoparticle probes with a simple reader coupled to a smartphone.

scholarly journals Computational Design of a Molecularly Imprinted Polymer for the Biomonitoring of the Organophosphorous Metabolite Chlorferron

Biosensors ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 192
Author(s):  
Bakhtiyar Qader ◽  
Issam Hussain ◽  
Mark Baron ◽  
Rebeca Jiménez-Pérez ◽  
Guzmán Gil-Ramírez ◽  
...  
Keyword(s):  
Biological Samples ◽  
Limit Of Detection ◽  
Computational Design ◽  
Signal Change ◽  
Highly Sensitive ◽  
Limit Of Quantitation ◽  
Molecularly Imprinting Polymers ◽  
Mip Sensor ◽  
Parasitic Mites

Coumaphos is an organophosphorus compound used as insecticide and frequently used by beekeepers for the management of parasitic mites. The most important metabolite, chlorferron (CFN), has been identified in biological samples and foodstuff. The need to quickly identify the presence of typical metabolites, as an indication of interaction with coumaphos has driven the need to produce a highly sensitive electrochemical method for chlorferron analysis, based on molecularly imprinting polymers (MIP) technology. It showed irreversible behaviour with mixed diffusion/adsorption-controlled reactions at the electrode surface. A monoelectronic mechanism of reaction for oxidation has also been suggested. The linear range observed was from 0.158 to 75 µM. Median precision in terms of %RSD around 3% was also observed. For DPV, the limit of detection (LOD) and the limit of quantitation (LOQ) for the CFN-MIP were 0.158 µM and 0.48 µM, respectively. The obtained median % recovery was around 98%. The results were also validated to reference values obtained using GC-MS. Urine and human synthetic plasma spiked with CFN were used to demonstrate the usability of the method in biological samples, showing the potential for biomonitoring. The developed imprinted sensor showed maximum signal change less than 16.8% when related metabolites or pesticide were added to the mix, suggesting high selectivity of the MIP sensor toward CFN molecules. The results from in vitro metabolism of CMP analysed also demonstrates the potential for detection and quantification of CFN in environmental samples. The newly developed CFN-MIP sensor offers similar LoDs than chromatographic methods with shorter analysis time.

scholarly journals Recent Advancements in Enzyme-Based Lateral Flow Immunoassays

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3358
Author(s):  
Donato Calabria ◽  
Maria Maddalena Calabretta ◽  
Martina Zangheri ◽  
Elisa Marchegiani ◽  
Ilaria Trozzi ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Qualitative Information ◽  
Eye Color ◽  
Commercial Success ◽  
Color Changes ◽  
Naked Eye ◽  
Future Developments ◽  
Highly Sensitive ◽  
Critical Overview ◽  
Detection Technologies

Paper-based lateral-flow immunoassays (LFIAs) have achieved considerable commercial success and their impact in diagnostics is continuously growing. LFIA results are often obtained by visualizing by the naked eye color changes in given areas, providing a qualitative information about the presence/absence of the target analyte in the sample. However, this platform has the potential to provide ultrasensitive quantitative analysis for several applications. Indeed, LFIA is based on well-established immunological techniques, which have known in the last year great advances due to the combination of highly sensitive tracers, innovative signal amplification strategies and last-generation instrumental detectors. All these available progresses can be applied also to the LFIA platform by adapting them to a portable and miniaturized format. This possibility opens countless strategies for definitively turning the LFIA technique into an ultrasensitive quantitative method. Among the different proposals for achieving this goal, the use of enzyme-based immunoassay is very well known and widespread for routine analysis and it can represent a valid approach for improving LFIA performances. Several examples have been recently reported in literature exploiting enzymes properties and features for obtaining significative advances in this field. In this review, we aim to provide a critical overview of the recent progresses in highly sensitive LFIA detection technologies, involving the exploitation of enzyme-based amplification strategies. The features and applications of the technologies, along with future developments and challenges, are also discussed.

scholarly journals Nanoimprinted multifunctional nanoprobes for a homogeneous immunoassay in a top-down fabrication approach

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hubert Brueckl ◽  
Astrit Shoshi ◽  
Stefan Schrittwieser ◽  
Barbara Schmid ◽  
Pia Schneeweiss ◽  
...  
Keyword(s):  
Thin Film ◽  
Nanoimprint Lithography ◽  
Thin Film Deposition ◽  
Film Deposition ◽  
In Vitro Diagnostics ◽  
Top Down ◽  
Highly Sensitive ◽  
Optical And Magnetic Properties ◽  
Lift Off

AbstractMultifunctional nanoparticles are discussed as versatile probes for homogeneous immunoassays for in-vitro diagnostics. Top-down fabrication allows to combine and tailor magnetic and plasmonic anisotropic properties. The combination of nanoimprint lithography, thin film deposition, and lift-off processing provides a top-down fabrication platform, which is both flexible and reliable. Here, we discuss the material compositions and geometrical designs of monodisperse multicomponent nanoparticles and their consequences on optical and magnetic properties. The rotational hydrodynamics of nanoparticles is measured and considered under the influence of magnetic shape anisotropy in the framework of the Stoner-Wohlfarth theory. The plasmon-optical properties are explained by discrete-dipole finite-element simulations. Rotational dynamical measurements of imprinted nanoprobes for two test proteins demonstrate the applicability as highly sensitive biomolecular nanoprobes.

scholarly journals Innovative and Highly Sensitive Detection of Clostridium perfringens Enterotoxin Based on Receptor Interaction and Monoclonal Antibodies

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 266
Author(s):  
Thea Neumann ◽  
Maren Krüger ◽  
Jasmin Weisemann ◽  
Stefan Mahrhold ◽  
Daniel Stern ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Clostridium Perfringens ◽  
Food Poisoning ◽  
Receptor Interaction ◽  
Clostridium Perfringens Enterotoxin ◽  
Fast Detection ◽  
Highly Sensitive ◽  
Basic And Applied Research ◽  
Highly Sensitive Detection

Clostridium perfringens enterotoxin (CPE) regularly causes food poisoning and antibiotic-associated diarrhea; therefore, reliable toxin detection is crucial. To this aim, we explored stationary and mobile strategies to detect CPE either exclusively by monoclonal antibodies (mAbs) or, alternatively, by toxin-enrichment via the cellular receptor of CPE, claudin-4, and mAb detection. Among the newly generated mAbs, we identified nine CPE-specific mAbs targeting five distinct epitopes, among them mAbs recognizing CPE bound to claudin-4 or neutralizing CPE activity in vitro. In surface plasmon resonance experiments, all mAbs and claudin-4 revealed excellent affinities towards CPE, ranging from 0.05 to 2.3 nM. Integrated into sandwich enzyme-linked immunosorbent assays (ELISAs), the most sensitive mAb/mAb and claudin-4/mAb combinations achieved similar detection limits of 0.3 pg/mL and 1.0 pg/mL, respectively, specifically detecting recombinant CPE from spiked feces and native CPE from 30 different C. perfringens culture supernatants. The implementation of mAb- and receptor-based ELISAs into a mobile detection platform enabled the fast detection of CPE, which will be helpful in clinical laboratories to diagnose diarrhea of assumed bacterial origin. In conclusion, we successfully employed an endogenous receptor and novel high affinity mAbs for highly sensitive and specific CPE-detection. These tools will be useful for both basic and applied research.

scholarly journals Optical properties of in-vitro biomineralised silica

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Alessandro Polini ◽  
Stefano Pagliara ◽  
Andrea Camposeo ◽  
Roberto Cingolani ◽  
Xiaohong Wang ◽  
...  
Keyword(s):  
Optical Properties

In-vivo and in-vitro study of control of rat skin optical properties by action of 40%-glucose solution

2001 ◽  
Author(s):  
Alexey N. Bashkatov ◽  
Elina A. Genina ◽  
Irina V. Korovina ◽  
Yurii P. Sinichkin ◽  
Olga V. Novikova ◽  
...  
Keyword(s):  
Optical Properties ◽  
Glucose Solution ◽  
In Vitro Study ◽  
Vitro Study ◽  
Rat Skin

Optical properties of selected native and coagulated human brain tissues in vitro in the visible and near infrared spectral range

2002 ◽  
Vol 47 (12) ◽  
pp. 2059-2073 ◽  
Author(s):  
A N Yaroslavsky ◽  
P C Schulze ◽  
I V Yaroslavsky ◽  
R Schober ◽  
F Ulrich ◽  
...  
Keyword(s):  
Optical Properties ◽  
Human Brain ◽  
Spectral Range ◽  
Near Infrared ◽  
Infrared Spectral Range ◽  
Infrared Spectral ◽  
Brain Tissues

Applications of Quantum Dots in Cancer Research

2011 ◽  
Vol 345 ◽  
pp. 29-34
Author(s):  
Xue Feng Wang ◽  
Jing Ding ◽  
Ji Yu Li ◽  
Han Jiang ◽  
Zi Hao Wang ◽  
...  
Keyword(s):  
Cancer Research ◽  
Quantum Dots ◽  
Optical Properties ◽  
Nanocrystalline Materials ◽  
Mammary Cancer ◽  
Cell Tracking ◽  
Bohr Radius ◽  
Quantum Size ◽  
Highly Sensitive ◽  
Diagnosis Rate

Quantum dots(QDs) usually refers to nanocrystalline materials whose diameter is smaller than the exciton Bohr radius. These materials have quantum size effect,the most significant manifestation is their optical properties change with particle size.The unique optical properties make quantum dots to be Ideal markers for tumor cell tracking and targeting,such as mammary cancer, liver cancer, and melanoma.There are broad prospects in tapping the potential of this highly sensitive technology in serum and other body fluids, so as to increase the early diagnosis rate of tumors.

scholarly journals Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 768
Author(s):  
Hyung-Mo Kim ◽  
Chiwoo Oh ◽  
Jaehyun An ◽  
Seungki Baek ◽  
Sungje Bock ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Calf Serum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Uniform Size ◽  
Highly Sensitive ◽  
Wide Range ◽  
New Biomarkers

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.

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