All-in-one microfluidic device for on-site diagnosis of pathogens based on an integrated continuous flow PCR and electrophoresis biochip

Lab on a Chip ◽  
2019 ◽  
Vol 19 (16) ◽  
pp. 2663-2668 ◽  
Author(s):  
Zhenqing Li ◽  
Ruixue Ju ◽  
Shinichi Sekine ◽  
Dawei Zhang ◽  
Songlin Zhuang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Dna Amplification ◽  
Syringe Pump ◽  
Pcr Products

A miniaturized device based on integrated continuous flow PCR and electrophoresis microfluidic chip was developed for diagnosis of pathogens. It dispensed with costly external precision syringe pump and can realize rapid DNA amplification and on-site PCR products detection.

Self-propelled continuous-flow PCR in capillary-driven microfluidic device: Microfluidic behavior and DNA amplification

2015 ◽  
Vol 206 ◽  
pp. 303-310 ◽  
Author(s):  
Hiroaki Tachibana ◽  
Masato Saito ◽  
Koji Tsuji ◽  
Keiichiro Yamanaka ◽  
Le Quynh Hoa ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Dna Amplification

scholarly journals Spiral Microfluidics Device for Continuous Flow PCR

2013 ◽  
Author(s):  
Reza Salemmilani ◽  
Barbaros Cetin
Keyword(s):  
Microfluidic Device ◽  
Continuous Flow ◽  
Biomedical Applications ◽  
Solution Process ◽  
Dna Amplification ◽  
High Rate ◽  
Future Research ◽  
Glass Wafer ◽  
Repeated Heating ◽  
Micro Channels

Polymerase-chain-Reaction (PCR) is a thermal cycling (repeated heating and cooling of PCR solution) process for DNA amplification. PCR is the key ingredient in many biomedical applications. One key feature for the success of the PCR is to control the temperature of the solution precisely at the desired temperature levels required for the PCR in a cyclic manner. Microfluidics offers a great advantage over conventional techniques since minute amounts of PCR solution can be heated and cooled with a high rate in a controlled manner. In this study, a microfluidic platform has been proposed for continuous-flow PCR. The microfluidic device consists of a spiral channel on a glass wafer with integrated chromium microheaters. Sub-micron thick microheaters are deposited beneath the micro-channels to facilitate localized heating. The microfluidic device is modeled using COMSOL Multiphysics®. The fabrication procedure of the device is also discussed and future research directions are addressed. With its compact design, the proposed system can easily be coupled with an integrated microfluidic device to be used in biomedical applications.

An extrusion fluidic driving method for continuous-flow polymerase chain reaction on a microfluidic chip

2009 ◽  
Vol 168 (1-2) ◽  
pp. 71-78 ◽  
Author(s):  
Zhang-Run Xu ◽  
Xin Wang ◽  
Xiao-Feng Fan ◽  
Jian-Hua Wang
Keyword(s):  
Polymerase Chain Reaction ◽  
Microfluidic Chip ◽  
Continuous Flow ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Continuous-Flow Synthesis of N-Succinimidyl 4-[18F]fluorobenzoate Using a Single Microfluidic Chip

PLoS ONE ◽  
2016 ◽  
Vol 11 (7) ◽  
pp. e0159303 ◽  
Author(s):  
Hiroyuki Kimura ◽  
Kenji Tomatsu ◽  
Hidekazu Saiki ◽  
Kenji Arimitsu ◽  
Masahiro Ono ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Continuous Flow ◽  
Flow Synthesis

Free Flow Isoelectric Focusing in a Microfluidic Device

2014 ◽  
Author(s):  
Kisoo Yoo ◽  
Prashanta Dutta ◽  
Jin Liu
Keyword(s):  
Mathematical Model ◽  
Isoelectric Focusing ◽  
Microfluidic Device ◽  
Microfluidic Chip ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Free Flow ◽  
Charged Species ◽  
Simulation Results ◽  
Separation Of Proteins

In recent years, there are growing interests in the use of free flow isoelectric focusing (FFIEF). In FFIEF, a thin sheath of laminar flow is introduced perpendicular to the direction of the applied electric field for continuous separation of proteins and charged species. This technique is especially useful in microfluidic device since the electrophoretically separated bands do not have to be mobilized for detection or further analysis. In this study, a mathematical model is developed to simulate free flow isoelectric process in microfluidic devices considering electroneutrality and incompressibility of electrolytes. Our mathematical model is based on mass, momentum and charge conservation equations. A finite volume based numerical scheme is implemented to simulate two dimensional FFIEF in a microfluidic chip. Simulation results indicate that pH gradient forms as samples flow downstream and proteins can be separated effectively using this technique. A new design of microfluidic chip is proposed for separation for cardiac troponin I from serum albumin using FFIEF technique.

Fabrication and Application of PMMA Continuous-Flow PCR Microfluidic Chip with CO2Laser Direct-Writing Ablation Micromachining Technique

2009 ◽  
Vol 36 (5) ◽  
pp. 1239-1245
Author(s):  
祁恒 祁恒 ◽  
王贤松 王贤松 ◽  
陈涛 陈涛 ◽  
马雪梅 马雪梅 ◽  
姚李英 姚李英 ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Continuous Flow ◽  
Direct Writing

Continuous-flow, well-mixed, microfluidic crystallization device for screening of polymorphs, morphology, and crystallization kinetics at controlled supersaturation

Lab on a Chip ◽  
2019 ◽  
Vol 19 (14) ◽  
pp. 2373-2382 ◽  
Author(s):  
Paria Coliaie ◽  
Manish S. Kelkar ◽  
Nandkishor K. Nere ◽  
Meenesh R. Singh
Keyword(s):  
Crystallization Kinetics ◽  
Microfluidic Device ◽  
Continuous Flow ◽  
Crystalline Materials ◽  
Screening Techniques

While the conventional screening techniques suffer from depletion of supersaturation, the continuous-flow microfluidic device screens crystalline materials at controlled supersaturation.

Optimization of Geometry for Continuous Flow PCR Devices in a Titer Plate-Based PCR Multi-Reactor Platform

2007 ◽  
Author(s):  
D. S. Park ◽  
P.-C. Chen ◽  
B. H. You ◽  
N. Kim ◽  
T. Park ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Dna Amplification ◽  
Genome Project ◽  
Large Area ◽  
Minimum Width ◽  
Structural Rigidity ◽  
Steady State Temperature ◽  
Custom Made ◽  
Thermal Cycler ◽  
The Human Genome Project

A highly parallel, polymerase chain reaction (PCR) multireactor platform is in high demand to satisfy the high throughput requirements for exploiting the accumulated genetic information from the Human Genome Project. By incorporating continuous flow PCR (CFPCR) devices in a polymer 96-well titer plate format, DNA amplification can be performed with steady-state temperature control and faster reaction speed at lower cost. Prior to the realization of a PCR multi-reactor platform, consisting of a sample delivery chip, a PCR multireactor chip, and a thermal cycler, optimization of the geometry for CFPCR devices in a titer plate-based PCR multi-reactor chip based on manufacturing feasibility is necessary. A prototype PCR multi-reactor chip was designed in a 96-well titer plate format with twelve different CFPCR configurations. High quality metallic, large area mold inserts (LAMIs) were fabricated using an SU-8 based UV-LIGA technique by overplating nickel in SU-8 electroplating templates. Micro molding of polycarbonate (PC) was done using hot embossing, resulting in good replication fidelity over the large surface area. Thermal fusion bonding of the molded PC chips using a custom-made bonding jig yielded acceptable sealing results. The manufacturability investigation throughout the design and the process sequence suggested that the microchannel walls require a minimum width of at least 20 μm and an aspect ratio of 2 for structural rigidity. An optimal CFPCR device for use in a PCR multi-reactor chip can be selected with a series of amplification experiments with the development of a thermal cycler.

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