Addition of flavonols and polysaccharides as excipient ingredients into epicatechin rich green tea extract inhibited free radical formation and glucose uptake

2020 ◽  
Vol 11 (4) ◽  
pp. 3105-3111
Author(s):  
So-Hee Yoo ◽  
Yeong-Eun Lee ◽  
Jin-Oh Chung ◽  
Chan-Su Rha ◽  
Yong-Deog Hong ◽  
...  
Keyword(s):  
Free Radical ◽  
Glucose Uptake ◽  
Green Tea ◽  
Green Tea Extract ◽  
Radical Formation ◽  
Tea Catechins ◽  
Green Tea Catechins ◽  
Free Radical Formation ◽  
Tea Extract

Results from the current study suggested that whole green tea components rich in flavonols and polysaccharides could be potential hypoglycemic excipient ingredients into green tea catechins by enhancing catechin absorption.

scholarly journals The impact of the catechol-O-methyltransferase genotype on the acute responsiveness of vascular reactivity to a green tea extract

2010 ◽  
Vol 105 (8) ◽  
pp. 1138-1144 ◽  
Author(s):  
Rosalind J. Miller ◽  
Kim G. Jackson ◽  
Tony Dadd ◽  
Andrew E. Mayes ◽  
A. Louise Brown ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Green Tea ◽  
Vascular Reactivity ◽  
Green Tea Extract ◽  
Genotypic Differences ◽  
Comt Genotype ◽  
Tea Catechins ◽  
Green Tea Catechins ◽  
Tea Extract ◽  
The Impact

The beneficial effects of green tea catechins, such as the proposed improvement in endothelial function, may be influenced by phase II metabolism during and after absorption. The methylation enzyme, catechol-O-methyltransferase (COMT), has a missense mutation rs4680 (G to A), proposed to result in a 40 % reduction in enzyme activity. In the present pilot study, twenty subjects (ten of each homozygous COMT genotype) were recruited. Green tea extract capsules (836 mg green tea catechins) were given in a fasted state, and a high-carbohydrate breakfast was given after 60 min. Blood samples and vascular function measurements were taken at regular intervals. The change in digital volume pulse stiffness index (SI) from baseline was shown to be different between genotype groups at 120 and 240 min, with a lower SI in the GG individuals (P ≤ 0·044). The change in blood pressure from baseline also differed between genotype groups, with a greater increase in systolic (P = 0·023) and diastolic (P = 0·034) blood pressure at 120 min in the GG group. The AA group was shown to have a greater increase in insulin concentrations at 120 min (P = 0·019) and 180 min (P = 0·008) compared with baseline, despite similar glucose profiles. No genotypic differences were found in vascular reactivity measured using laser Doppler iontophoresis, total nitrite, lipids, plasma total antioxidant capacity or inflammatory markers after ingestion of the green tea extract. In conclusion, SI and insulin response to the glucose load differed between the COMT genotype groups, and this may be suggestive of a green tea extract and genotype interaction.

scholarly journals Molecular Rescue of Dyrk1A Overexpression Alterations in Mice with Fontup® Dietary Supplement: Role of Green Tea Catechins

2020 ◽  
Vol 21 (4) ◽  
pp. 1404 ◽  
Author(s):  
Yuchen Gu ◽  
Gautier Moroy ◽  
Jean-Louis Paul ◽  
Anne-Sophie Rebillat ◽  
Mara Dierssen ◽  
...  
Keyword(s):  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Nutritional Supplement ◽  
Green Tea Extract ◽  
Epicatechin Gallate ◽  
Tea Catechins ◽  
Green Tea Catechins ◽  
Tea Extract ◽  
Vitro Effect

Epigallocatechin gallate (EGCG) is an inhibitor of DYRK1A, a serine/threonine kinase considered to be a major contributor of cognitive dysfunctions in Down syndrome (DS). Two clinical trials in adult patients with DS have shown the safety and efficacy to improve cognitive phenotypes using commercial green tea extract containing EGCG (45% content). In the present study, we performed a preclinical study using FontUp®, a new nutritional supplement with a chocolate taste specifically formulated for the nutritional needs of patients with DS and enriched with a standardized amount of EGCG in young mice overexpressing Dyrk1A (TgBACDyrk1A). This preparation is differential with previous one used, because its green tea extract has been purified to up 94% EGCG of total catechins. We analyzed the in vitro effect of green tea catechins not only for EGCG, but for others residually contained in FontUp®, on DYRK1A kinase activity. Like EGCG, epicatechin gallate was a noncompetitive inhibitor against ATP, molecular docking computations confirming these results. Oral FontUp® normalized brain and plasma biomarkers deregulated in TgBACDyrk1A, without negative effect on liver and cardiac functions. We compared the bioavailability of EGCG in plasma and brain of mice and have demonstrated that EGCG had well crossed the blood-brain barrier.

Synthesis and Antioxidant Activity of Resin of Chitosan Cerium Encapsulated Green Tea Extract Microspheres

2011 ◽  
Vol 194-196 ◽  
pp. 734-741
Author(s):  
Li Na Yu ◽  
Dong Feng Wang ◽  
Qing Li Yang ◽  
Hai Yan Li ◽  
Bing Jie Liu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Green Tea ◽  
Polyphenolic Compounds ◽  
Green Tea Extract ◽  
Emission Spectrometry ◽  
Narrow Particle Size Distribution ◽  
Human Beings ◽  
Free Radical Damage ◽  
Tea Extract

The purpose of this paper is to investigate a new type of resin of chitosan cerium encapsulated green tea extract microspheres (RCCM-GTE) prepared by the reversed-phase suspension cross-linking polymerization and study the scavenging activity against DPPH free radical. The results showed that RCCM-GTE was a type of deep brown-yellow spherical resin with smooth surface and it presented uniform and narrow particle size distribution as determined by the Laser Particles Sizer. The Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES), Fourier Transform Infrared Spectroscopy (FT-IR) and differential scanning calorimetry (DSC) study demonstrated that there was cerium existing in RCCM-GTE. The polyphenolic compounds existed in RCCM-GTE and the total content of polyphenolic compounds encapsulated in RCCM-GTE was found to be 92.550±1.145 μg/g. It is considered that the good antioxidant activity of RCCM-GTE was the comprehensive results of the action of tea polysaccharide-protein conjugate, polyphenolic compounds, cerium and Schiff base chitosan. Investigation showed that a lot of extracts coming from agriculture and food industry contain reactive components of polyphenolic compounds and polysaccharides. It can be effective in protection of human beings from free radical damage if these extracts are directly or after the extraction and purification added to food as natural food antioxidant or they are prepared micospheres reacted with some biopolymers through coordination as a health food. Then the emerging area of research on encapsulating GTE in chitosan cerium resin must have a broad developing prospect.

scholarly journals Green Tea Extract Concurrent with an Oral Nutritional Supplement Acutely Enhances Muscle Microvascular Blood Flow without Altering Leg Glucose Uptake in Healthy Older Adults

Nutrients ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 3895
Author(s):  
Ushnah S. U. Din ◽  
Tanvir S. Sian ◽  
Colleen S. Deane ◽  
Ken Smith ◽  
Amanda Gates ◽  
...  
Keyword(s):  
Older Adults ◽  
Blood Flow ◽  
Glucose Uptake ◽  
Green Tea ◽  
Nutritional Supplement ◽  
Green Tea Extract ◽  
Microvascular Blood Flow ◽  
Healthy Older Adults ◽  
Oral Nutritional Supplement ◽  
Tea Extract

Postprandial macro- and microvascular blood flow and metabolic dysfunction manifest with advancing age, so vascular transmuting interventions are desirable. In this randomised, single-blind, placebo-controlled, crossover trial, we investigated the impact of the acute administration of green tea extract (GTE; containing ~500 mg epigallocatechin-3-gallate) versus placebo (CON), alongside an oral nutritional supplement (ONS), on muscle macro- and microvascular, cerebral macrovascular (via ultrasound) and leg glucose/insulin metabolic responses (via arterialised/venous blood samples) in twelve healthy older adults (42% male, 74 ± 1 y). GTE increased m. vastus lateralis microvascular blood volume (MBV) at 180 and 240 min after ONS (baseline: 1.0 vs. 180 min: 1.11 ± 0.02 vs. 240 min: 1.08 ± 0.04, both p < 0.005), with MBV significantly higher than CON at 180 min (p < 0.05). Neither the ONS nor the GTE impacted m. tibialis anterior perfusion (p > 0.05). Leg blood flow and vascular conductance increased, and vascular resistance decreased similarly in both conditions (p < 0.05). Small non-significant increases in brachial artery flow-mediated dilation were observed in the GTE only and middle cerebral artery blood flow did not change in response to GTE or CON (p > 0.05). Glucose uptake increased with the GTE only (0 min: 0.03 ± 0.01 vs. 35 min: 0.11 ± 0.02 mmol/min/leg, p = 0.007); however, glucose area under the curve and insulin kinetics were similar between conditions (p > 0.05). Acute GTE supplementation enhances MBV beyond the effects of an oral mixed meal, but this improved perfusion does not translate to increased leg muscle glucose uptake in healthy older adults.

scholarly journals Development and Validation of a HPLC Method of Quantification of Caffeine and EGCG in Green Tea (Camellia sinensis L.) Extract

2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Tra Trong Bien ◽  
Bui Thi Lan Phuong
Keyword(s):  
Green Tea ◽  
Camellia Sinensis ◽  
High Performance ◽  
Protective Role ◽  
Hplc Method ◽  
Green Tea Extract ◽  
Ultrahigh Pressure ◽  
Bioactive Constituents ◽  
Tea Catechins ◽  
Tea Extract

This study investigates a simple high performance liquid chromatography (HPLC) in an isocratic eluent manner for quantification of caffeine and EGCG in green tea extract. The study separation system contained a C18-RP column (250 × 4.6 mm, 5 µm), a mobile phase of methanol/phosphoric acid/water (20/0.1/79.9, v/v) and a DAD detector at 280 nm. This system proves convenient for rapid routine analysis of caffeine and EGCG in green tea extract with good repeatability and accuracy. Keywords HPLC, caffeine, EGCG, green tea, extract. References [1] A. Chowdhury, J. Sarkar, T. Chakraborti, P.K. Pramanik, S. Chakraborti, Protective role of epigallocatechin-3-gallate in health and disease: A perspective, Biomedicine & Pharmacotherapy 78 (2016) 50-59. https://doi.org/10.1016/j.biopha.2015.12.013.[2] Y. Hara, Tea catechins and their applications as supplements and pharmaceutics, Pharmacological Research 64(2) (2011) 100-104. https://doi.org/10.1016/j.phrs.2011.03.018.[3] X. Jun, S. Zhao, B. Lu, R. Zhang, Y. Li, D. Shen, G. Zhou, Separation of major catechins from green tea by ultrahigh pressure extraction, International Journal of Pharmaceutics 386(1-2) (2010) 229-231. https://doi.org/10.1016/j.ijpharm.2009.10.035.[4] Z. Bedrood, M. Rameshrad, H. Hosseinzadeh, Toxicological effects of Camellia sinensis (green tea): A review, Phytotherapy Research 32(7) (2018) 1163-1180. https://doi.org/10.1002/ptr.6063.[5] J. Michael Glade, Caffeine-Not just a stimulant, Nutrition 26(10) (2010) 932-938. https://doi.org/10.1016/j.nut.2010.08.004.[6] MediGene AG. Press Release., MediGene AG Obtains Approval of Polyphenon®E Ointment in the USA, 2006.[7] Q.V. Vuong, V. Nguyen, J.B. Golding, P.D. Roach, The content of bioactive constituents as a quality index for Vietnamese teas, International Food Research Journal 18(1) (2011) 329-336.[8] Tran Trong Bien, Method for caffeine removal and catechins enrichment from green tea leaf, Master thesis, Hanoi University of Pharmacy, 2019 (In Vietnamese).[9] J. Joseph Dalluge, C. Bryant Nelson, Determination of tea catechins, Journal of Chromatography A 881 (2000) 411–424. https://doi.org/10.1016/S0021-9673(00)00062-5.[10] T. Shiono, et al., Selective decaffeination of tea extracts by montmorillonite, Journal of Food Engineering 200 (2017) 13-21. https://doi.org/10.1016/j.jfoodeng.2016.12.015.    

Green Tea Extract Exposure Pattern Differenzially Affects Acetaminophen-Induced Hepatotoxicity

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
A Ali ◽  
X Yang ◽  
Q Shi ◽  
J Greenhaw ◽  
WF Salminen
Keyword(s):  
Green Tea ◽  
Green Tea Extract ◽  
Exposure Pattern ◽  
Tea Extract
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