Click chemistry-based imaging to study the tissue distribution of the curcumin–protein complex in mice

2020 ◽  
Vol 11 (2) ◽  
pp. 1684-1691
Author(s):  
Jingyi Zhou ◽  
Weicang Wang ◽  
Jianan Zhang ◽  
Zheyuan Du ◽  
Haixia Yang ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Protein Complex ◽  
Protein Thiols ◽  
Modify Protein ◽  
Liver Tissues

Using click chemistry-based fluorescence imaging, here we show that curcumin, a bioactive dietary compound with a thiol-reactive α,β-unsaturated carbonyl moiety, can covalently modify protein thiols in colon and liver tissues in mice.

scholarly journals Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants

Plant Methods ◽  
2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Janet M. Paper ◽  
Thiya Mukherjee ◽  
Kathrin Schrick
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging

Whole-body section fluorescence imaging - a novel method for tissue distribution studies of fluorescent substances

2009 ◽  
Vol 4 (2) ◽  
pp. 73-80 ◽  
Author(s):  
Siver Moestue ◽  
Paula Nunez ◽  
Andrew Healey ◽  
Roger Bjerke ◽  
Bård Indrevoll ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Fluorescence Imaging ◽  
Whole Body ◽  
Novel Method ◽  
Distribution Studies ◽  
Body Section

scholarly journals Novel vinyl-modified RGD conjugated silica nanoparticles based on photo click chemistry for in vivo prostate cancer targeted fluorescence imaging

RSC Advances ◽  
2019 ◽  
Vol 9 (44) ◽  
pp. 25318-25325
Author(s):  
Hanrui Li ◽  
Ke Li ◽  
Qi Zeng ◽  
Yun Zeng ◽  
Dan Chen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Click Chemistry ◽  
Silica Nanoparticles ◽  
Fluorescence Imaging ◽  
Tumor Targeting ◽  
Diagnosis And Treatment ◽  
Targeting Ability

Photo click chemistry has been used to prepare RGD conjugated silica nanoprobe (SiO2@T1-RGDk NPs) that exhibits excellent tumor targeting ability and negligible toxicity which enables them to be used for the diagnosis and treatment of cancer.

scholarly journals Supported core–shell nanobiosensors for quantitative fluorescence imaging of extracellular pH

2014 ◽  
Vol 50 (89) ◽  
pp. 13746-13749 ◽  
Author(s):  
Jérémie Asselin ◽  
Carl Roy ◽  
Denis Boudreau ◽  
Younès Messaddeq ◽  
Rihab Bouchareb ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Ph Sensitive ◽  
Extracellular Ph ◽  
Live Cells ◽  
Core Shell ◽  
Spatially Resolved ◽  
Quantitative Fluorescence ◽  
Silica Substrates

“Click” chemistry was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent ion-sensitive surfaces for quantitative and spatially-resolved extracellular measurements on live cells.

scholarly journals Evaluation of the Hepatotoxicity of the Zhi-Zi-Hou-Po Decoction by Combining UPLC-Q-Exactive-MS-Based Metabolomics and HPLC-MS/MS-Based Geniposide Tissue Distribution

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 511 ◽  
Author(s):  
Yunji Wang ◽  
Fang Feng
Keyword(s):  
Tissue Distribution ◽  
Acid Metabolism ◽  
Bile Acid Metabolism ◽  
Sprague Dawley ◽  
Gardenia Jasminoides ◽  
Sprague Dawley Rats ◽  
Q Exactive ◽  
Serum Biochemical ◽  
Endogenous Metabolites ◽  
Liver Tissues

With traditional Chinese medicine (TCM) becoming widespread globally, its safety has increasingly become a concern, especially its hepatoxicity. For example, Gardenia jasminoides Ellis is a key ingredient in the Zhi-Zi-Hou-Po decoction (ZZHPD), which is a commonly-used clinically combined prescription of TCM that may induce hepatoxicity. However, the underlying toxicity mechanism of ZZHPD is not fully understood. In this study, a plasma metabolomics strategy was used to investigate the mechanism of ZZHPD-induced hepatotoxicity through profiling entire endogenous metabolites. Twenty-four Sprague-Dawley rats were randomly assigned into four groups, which were orally administered with 0.9% saline, as well as 2.7 g/kg/day, 8.1 g/kg/day, or 27 g/kg/day of ZZHPD for 30 consecutive days, respectively. Biochemical assay and metabolomics assay were used to detect serum and plasma samples, whilst histopathological assay was used for detecting liver tissues, and the geniposide distribution in tissues was simultaneously measured. The results showed that the concentration of 20 metabolites linked to amino acid, lipid, and bile acid metabolism had significant changes in the ZZHPD-treated rats. Moreover, toxic effects were aggravated with serum biochemical and histopathological examines in liver tissues as the dosage increased, which may be associated with the accumulation of geniposide in the liver as the dosage increased. Notably, our findings also demonstrated that the combined metabolomics strategy with tissue distribution had significant potential for elucidating the mechanistic complexity of the toxicity of TCM.

scholarly journals Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method

2016 ◽  
Vol 7 (9) ◽  
pp. 6182-6189 ◽  
Author(s):  
Xiaoru Zhang ◽  
Ruijuan Li ◽  
Yuanyuan Chen ◽  
Shusheng Zhang ◽  
Wenshuang Wang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Rolling Circle Amplification ◽  
Detection Sensitivity ◽  
Rolling Circle ◽  
Improve Detection Sensitivity ◽  
Cell Surface Glycans

We report herein imaging cell surface glycosylation by using click chemistry and DNA rolling circle amplification (RCA) to improve detection sensitivity..

scholarly journals Fluorescence imaging of chromosomal DNA using click chemistry

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Takumi Ishizuka ◽  
Hong Shan Liu ◽  
Kenichiro Ito ◽  
Yan Xu
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Chromosomal Dna

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

High-Resolution electron crystallography of the light-harvesting chlorophyll-a/b protein complex from chloroplast membranes

1990 ◽  
Vol 48 (1) ◽  
pp. 610-610
Author(s):  
Werner Kühlbrandt ◽  
Da Neng Wang ◽  
K.H. Downing
Keyword(s):  
High Resolution ◽  
Electron Diffraction ◽  
Chlorophyll A ◽  
Protein Complex ◽  
Structural Integrity ◽  
Light Harvesting ◽  
Lhc Ii ◽  
Diffraction Patterns ◽  
Difference Fourier ◽  
2D Crystals

The light-harvesting chlorophyll-a/b protein complex (LHC-II) is the most abundant membrane protein in the chloroplasts of green plants where it functions as a molecular antenna of solar energy for photosynthesis. We have grown two-dimensional (2d) crystals of the purified, detergent-solubilized LHC-II . The crystals which measured 5 to 10 μm in diameter were stabilized for electron microscopy by washing with a 0.5% solution of tannin. Electron diffraction patterns of untilted 2d crystals cooled to 130 K showed sharp spots to 3.1 Å resolution. Spot-scan images of 2d crystals were recorded at 160 K with the Berkeley microscope . Images of untilted crystals were processed, using the unbending procedure by Henderson et al . A projection map of the complex at 3.7Å resolution was generated from electron diffraction amplitudes and high-resolution phases obtained by image processing .A difference Fourier analysis with the same image phases and electron diffraction amplitudes recorded of frozen, hydrated specimens showed no significant differences in the 3.7Å projection map. Our tannin treatment therefore does not affect the structural integrity of the complex.

Use of Antibody to aid Visualization of Protein at the Termini of Bacteriophage Ø29 DNA

1980 ◽  
Vol 38 ◽  
pp. 540-541
Author(s):  
Dwight Anderson ◽  
Charlene Peterson ◽  
Gursaran Notani ◽  
Bernard Reilly
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Dna Synthesis ◽  
Restriction Endonuclease ◽  
Protein Complex ◽  
Protein Product ◽  
Viral Dna ◽  
Small Proteins ◽  
Antibody Labeling ◽  
Subtilis Bacteriophage

The protein product of cistron 3 of Bacillus subtilis bacteriophage Ø29 is essential for viral DNA synthesis and is covalently bound to the 5’-termini of the Ø29 DNA. When the DNA-protein complex is cleaved with a restriction endonuclease, the protein is bound to the two terminal fragments. The 28,000 dalton protein can be visualized by electron microscopy as a small dot and often is seen only when two ends are in apposition as in multimers or in glutaraldehyde-fixed aggregates. We sought to improve the visibility of these small proteins by use of antibody labeling.

Sign in / Sign up

Export Citation Format

Share Document