scholarly journals Fluorescence imaging of chromosomal DNA using click chemistry

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Takumi Ishizuka ◽  
Hong Shan Liu ◽  
Kenichiro Ito ◽  
Yan Xu
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Chromosomal Dna

scholarly journals Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants

Plant Methods ◽  
2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Janet M. Paper ◽  
Thiya Mukherjee ◽  
Kathrin Schrick
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging

scholarly journals Novel vinyl-modified RGD conjugated silica nanoparticles based on photo click chemistry for in vivo prostate cancer targeted fluorescence imaging

RSC Advances ◽  
2019 ◽  
Vol 9 (44) ◽  
pp. 25318-25325
Author(s):  
Hanrui Li ◽  
Ke Li ◽  
Qi Zeng ◽  
Yun Zeng ◽  
Dan Chen ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Click Chemistry ◽  
Silica Nanoparticles ◽  
Fluorescence Imaging ◽  
Tumor Targeting ◽  
Diagnosis And Treatment ◽  
Targeting Ability

Photo click chemistry has been used to prepare RGD conjugated silica nanoprobe (SiO2@T1-RGDk NPs) that exhibits excellent tumor targeting ability and negligible toxicity which enables them to be used for the diagnosis and treatment of cancer.

scholarly journals Supported core–shell nanobiosensors for quantitative fluorescence imaging of extracellular pH

2014 ◽  
Vol 50 (89) ◽  
pp. 13746-13749 ◽  
Author(s):  
Jérémie Asselin ◽  
Carl Roy ◽  
Denis Boudreau ◽  
Younès Messaddeq ◽  
Rihab Bouchareb ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Ph Sensitive ◽  
Extracellular Ph ◽  
Live Cells ◽  
Core Shell ◽  
Spatially Resolved ◽  
Quantitative Fluorescence ◽  
Silica Substrates

“Click” chemistry was used to functionalize silica substrates with pH-sensitive nanoparticles, thus producing uniform and highly luminescent ion-sensitive surfaces for quantitative and spatially-resolved extracellular measurements on live cells.

Click chemistry-based imaging to study the tissue distribution of the curcumin–protein complex in mice

2020 ◽  
Vol 11 (2) ◽  
pp. 1684-1691
Author(s):  
Jingyi Zhou ◽  
Weicang Wang ◽  
Jianan Zhang ◽  
Zheyuan Du ◽  
Haixia Yang ◽  
...  
Keyword(s):  
Tissue Distribution ◽  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Protein Complex ◽  
Protein Thiols ◽  
Modify Protein ◽  
Liver Tissues

Using click chemistry-based fluorescence imaging, here we show that curcumin, a bioactive dietary compound with a thiol-reactive α,β-unsaturated carbonyl moiety, can covalently modify protein thiols in colon and liver tissues in mice.

scholarly journals Applying DNA rolling circle amplification in fluorescence imaging of cell surface glycans labeled by a metabolic method

2016 ◽  
Vol 7 (9) ◽  
pp. 6182-6189 ◽  
Author(s):  
Xiaoru Zhang ◽  
Ruijuan Li ◽  
Yuanyuan Chen ◽  
Shusheng Zhang ◽  
Wenshuang Wang ◽  
...  
Keyword(s):  
Cell Surface ◽  
Click Chemistry ◽  
Fluorescence Imaging ◽  
Rolling Circle Amplification ◽  
Detection Sensitivity ◽  
Rolling Circle ◽  
Improve Detection Sensitivity ◽  
Cell Surface Glycans

We report herein imaging cell surface glycosylation by using click chemistry and DNA rolling circle amplification (RCA) to improve detection sensitivity..

Super-resolution fluorescence imaging of chromosomal DNA

2012 ◽  
Vol 177 (2) ◽  
pp. 344-348 ◽  
Author(s):  
Patrick J.M. Zessin ◽  
Kieran Finan ◽  
Mike Heilemann
Keyword(s):  
Fluorescence Imaging ◽  
Super Resolution ◽  
Chromosomal Dna

The influence of confocal microscope design on imaging performance

1993 ◽  
Vol 51 ◽  
pp. 144-145
Author(s):  
C J R Sheppard
Keyword(s):  
Image Quality ◽  
Fluorescence Imaging ◽  
Confocal Microscope ◽  
Industrial Applications ◽  
Three Dimensions ◽  
Design Parameters ◽  
Weak Signals ◽  
Size And Shape ◽  
Imaging Performance ◽  
Fundamental Limitation

The confocal microscope is now widely used in both biomedical and industrial applications for imaging, in three dimensions, objects with appreciable depth. There are now a range of different microscopes on the market, which have adopted a variety of different designs. The aim of this paper is to explore the effects on imaging performance of design parameters including the method of scanning, the type of detector, and the size and shape of the confocal aperture.It is becoming apparent that there is no such thing as an ideal confocal microscope: all systems have limitations and the best compromise depends on what the microscope is used for and how it is used. The most important compromise at present is between image quality and speed of scanning, which is particularly apparent when imaging with very weak signals. If great speed is not of importance, then the fundamental limitation for fluorescence imaging is the detection of sufficient numbers of photons before the fluorochrome bleaches.

Ubiquinone-BODIPY nanoparticles for tumor redox-responsive fluorescence imaging and photodynamic activity

2021 ◽  
Author(s):  
Byunghee Hwang ◽  
Tae-Il Kim ◽  
Hyunjin Kim ◽  
Sungjin Jeon ◽  
Yongdoo Choi ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Selective Activation ◽  
Turn On ◽  
Intracellular Environment ◽  
Redox Responsive ◽  
Photodynamic Activity

A ubiquinone-BODIPY photosensitizer self-assembles into nanoparticles (PS-Q-NPs) and undergoes selective activation within the highly reductive intracellular environment of tumors, resulting in “turn-on” fluorescence and photosensitizing activities.

Sign in / Sign up

Export Citation Format

Share Document