Immunomodulatory effects of an acidic polysaccharide fraction from herbal Gynostemma pentaphyllum tea in RAW264.7 cells

2019 ◽  
Vol 10 (4) ◽  
pp. 2186-2197 ◽  
Author(s):  
Daoyuan Ren ◽  
Yan Zhao ◽  
Quan Zheng ◽  
Aamina Alim ◽  
Xingbin Yang
Keyword(s):  
Molecular Weight ◽  
Raw264.7 Cells ◽  
Immunomodulatory Effects ◽  
Gynostemma Pentaphyllum ◽  
Polysaccharide Fraction ◽  
Acidic Polysaccharide

A new acidic polysaccharide (GPTP-3) with a molecular weight of 2.49 × 106 Da was extracted and purified from Gynostemma pentaphyllum tea.

scholarly journals Immunomodulatory Activity of Low Molecular-Weight Peptides from Nibea japonica in RAW264.7 Cells via NF-κB Pathway

Marine Drugs ◽  
2019 ◽  
Vol 17 (7) ◽  
pp. 404 ◽  
Author(s):  
Zhuangwei Zhang ◽  
Xuyang Hu ◽  
Lin Lin ◽  
Guofang Ding ◽  
Fangmiao Yu
Keyword(s):  
Nitric Oxide ◽  
Molecular Weight ◽  
Cell Cycle Progression ◽  
Protein Hydrolysate ◽  
Raw264.7 Cells ◽  
Immunomodulatory Activity ◽  
Low Molecular Weight ◽  
Phagocytic Capacity ◽  
Activated State ◽  
Factor Α

In this study, a low molecular-weight (Mw) peptide named NJP (<1 kDa), was purified from a protein hydrolysate of Nibea japonica by ultrafiltration, and its immunomodulatory effect on RAW264.7 cells was evaluated. The lactate dehydrogenase (LDH) and MTT assays were performed to explore the cytotoxicity of NJP. The results showed that NJP promoted cell proliferation and had no significant toxic effects on RAW264.7 cells. Moreover, the cells formed multiple pseudopodia indicating that they were in activated state. Further tests showed that NJP significantly promoted phagocytic capacity, and the secretion of proinflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β). It also increased the synthesis of nitric oxide (NO) by upregulating inducible nitric oxide synthase (iNOS) protein level. Flow cytometry revealed that NJP promoted cell cycle progression and increased the percentage of cells in G0/G1 phase. NJP promoted IκBα degradation, p65 and nuclear factor (NF)-κB activation and translocation by up-regulating IKKα/β protein expression. In conclusion, these results indicated that NJP exerts immunomodulatory effects on RAW264.7 cells through the NF-κB signaling pathway. Therefore, NJP can be incorporated in the production of functional foods or nutraceuticals.

Ginseng marc-derived low-molecular weight oligosaccharide inhibits the growth of skin melanoma cells via activation of RAW264.7 cells

2015 ◽  
Vol 29 (2) ◽  
pp. 344-353 ◽  
Author(s):  
Jeong Yeon Seo ◽  
Chang Won Lee ◽  
Doo Jin Choi ◽  
Jisun Lee ◽  
Jae Yeon Lee ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Melanoma Cells ◽  
Raw264.7 Cells ◽  
Low Molecular Weight ◽  
Skin Melanoma

scholarly journals P274 IMMUNOMODULATORY EFFECTS OF A LOW MOLECULAR WEIGHT HEPARIN (CB-01-05)

2008 ◽  
Vol 2 (1) ◽  
pp. 85
Author(s):  
C. Rosette ◽  
L. Moro ◽  
G. Celasco ◽  
R. Bozzella ◽  
M. Ajani ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight Heparin ◽  
Low Molecular Weight ◽  
Immunomodulatory Effects

scholarly journals Immunomodulatory Effects of Lycium barbarum Polysaccharide Extract and Its Uptake Behaviors at the Cellular Level

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1351 ◽  
Author(s):  
Le Feng ◽  
Xiao Xiao ◽  
Jing Liu ◽  
Junyan Wang ◽  
Nan Zhang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Biological Activities ◽  
Cell Monolayer ◽  
Intestinal Transport ◽  
Cellular Level ◽  
Cell Polarization ◽  
Raw264.7 Cells ◽  
Immunomodulatory Effects ◽  
Lycium Barbarum ◽  
Wide Range

Lycium barbarum L. is a widely used functional food and medicinal herb in Asian countries. L. barbarium polysaccharides (LBP) are considered as one of the major medicinal components of L. barbarium fruit and exhibits a wide range of biological activities. Here, we investigated the immunomodulatory effects of LBP and its uptake behaviors at the cellular level. LBP was prepared by water extraction and ethanol precipitation, and divided into two fractions based on the molecular weight distribution by ultrafiltration (LBP > 10 kDa and LBP < 10 kDa). The physicochemical properties of LBP and LBP fractions were well characterized. The LBP > 10 kDa fraction greatly enhanced the viability of macrophages RAW264.7 cells and induced cell polarization, but had weak effects to other tested tumor cell lines and normal cell line. This fraction could regulate the production of NO, TNF-α, IL-6 and ROS in RAW264.7 cells, suggesting both pro-inflammatory and anti-inflammatory effects. The dye-labeled LBP could be internalized into all tested cell lines and accumulated in lysosomes. The internalization of LBP in RAW264.7 cells is mainly through the clathrin-mediated endocytosis pathway. The Caco-2 intestinal transport experiment demonstrated that the dye labeled LBP could be transported through the Caco-2 cell monolayer (mimic intestinal epithelium) through clathrin-mediated endocytosis. These results demonstrate the immunomodulatory effects of LBP and its effective uptake by macrophages and intestine.

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