Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system

2020 ◽  
Vol 10 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Zeyu Yang ◽  
Hengwei Fu ◽  
Wenjie Ye ◽  
Youyu Xie ◽  
Qinghai Liu ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Asymmetric Synthesis ◽  
Environmentally Friendly ◽  
Cofactor Regeneration ◽  
Chiral Alcohols ◽  
Regeneration System ◽  
High Substrate ◽  
System A ◽  
System P

Based on substrate-coupled cofactor regeneration system, a high 2-propanol tolerance SmADH2 together with TBCR system can synthesise structurally diverse chiral alcohols at a high substrate loading with only 1.25 equivalents of 2-propanol.

scholarly journals Demethylation of vanillic acid by recombinant LigM in a one-pot cofactor regeneration system

2016 ◽  
Vol 6 (21) ◽  
pp. 7729-7737 ◽  
Author(s):  
Elena Rosini ◽  
Paola D'Arrigo ◽  
Loredano Pollegioni
Keyword(s):  
Vanillic Acid ◽  
Cofactor Regeneration ◽  
Methionine Synthase ◽  
Regeneration System ◽  
One Pot ◽  
Cofactor Recycling ◽  
System P

Recombinant LigM from Sphingobium SYK-6 and plant methionine synthase MetE enzyme efficiently convert vanillic acid into PCA with cofactor recycling.

scholarly journals A Boom Energy Regeneration System of Hybrid Hydraulic Excavator Using Energy Conversion Components

Actuators ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Tri Cuong Do ◽  
Duc Giap Nguyen ◽  
Tri Dung Dang ◽  
Kyoung Kwan Ahn
Keyword(s):  
Management Strategy ◽  
Electrical Energy ◽  
Maximum Energy ◽  
Regeneration System ◽  
Hydraulic Pump ◽  
Energy Management Strategy ◽  
Energy Regeneration ◽  
System A ◽  
Time Optimal ◽  
Novel Design

In this paper, a novel design of an energy regeneration system was proposed for recovering as well as reusing potential energy in a boom cylinder. The proposed system included a hydraulic pump/motor and an electrical motor/generator. When the boom moved down, the energy regeneration components converted the hydraulic energy to electrical energy and stored in a battery. Then, the regenerated energy was reused at subsequent cycles. In addition, an energy management strategy has been designed based on discrete time-optimal control to guarantee position tracking performance and ensure component safety during the operation. To verify the effectiveness of the proposed system, a co-simulation (using MATLAB and AMESim) was carried out. Through the simulation results, the maximum energy regeneration efficiency could achieve up to 44%. Besides, the velocity and position of the boom cylinder achieved good performance with the proposed control strategy.

Asymmetric Synthesis of Organophosphorus Compounds using H-P reagents derived from Chiral Alcohols

2021 ◽  
Author(s):  
Tomasz K. Olszewski ◽  
Joseph Gbubele
Keyword(s):  
Asymmetric Synthesis ◽  
Phosphorus Atom ◽  
Organophosphorus Compounds ◽  
Chiral Alcohols

Chiral organophosphorus compounds, especially those containing C-stereogenic carbons in the proximity of the phosphorus atom, are known for their unique properties and have found wide applications that span from medicinal...

Direct conversion of cellulose to ethyl levulinate catalysed by modified fibrous mesoporous silica nanospheres in a co-solvent system

2021 ◽  
Vol 45 (12) ◽  
pp. 5526-5539
Author(s):  
Zhi Zhang ◽  
Zhihang Huang ◽  
Hong Yuan
Keyword(s):  
Mesoporous Silica ◽  
Solvent System ◽  
Direct Conversion ◽  
Reaction Yield ◽  
Silica Nanospheres ◽  
Ethyl Levulinate ◽  
System A ◽  
System P ◽  
Mesoporous Silica Nanospheres

A KCC-1/Al–SO3H catalyst with Si/Al = 5 was prepared to directly catalyse the synthesis of ethyl levulinate from cellulose in an ethanol/toluene co-solvent system. A reaction yield of 28.8 mol% was achieved after 6 h at 200 °C.

Cell-Free Total Biosynthesis of Plant Terpene Natural Products Using an Orthogonal Cofactor Regeneration System

ACS Catalysis ◽  
2021 ◽  
pp. 9898-9903
Author(s):  
Undramaa Bat-Erdene ◽  
John M. Billingsley ◽  
William C. Turner ◽  
Benjamin R. Lichman ◽  
Francesca M. Ippoliti ◽  
...  
Keyword(s):  
Natural Products ◽  
Cofactor Regeneration ◽  
Regeneration System

scholarly journals Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Langxing Liao ◽  
Yonghui Zhang ◽  
Yali Wang ◽  
Yousi Fu ◽  
Aihui Zhang ◽  
...  
Keyword(s):  
Glucose Dehydrogenase ◽  
Catalytic Efficiency ◽  
Space Time ◽  
Cofactor Regeneration ◽  
Enzyme Complex ◽  
Regeneration System ◽  
Regeneration Rate ◽  
Leucine Dehydrogenase ◽  
Fusion Enzyme ◽  
Space Time Yield

Abstract Background Biosynthesis of l-tert-leucine (l-tle), a significant pharmaceutical intermediate, by a cofactor regeneration system friendly and efficiently is a worthful goal all the time. The cofactor regeneration system of leucine dehydrogenase (LeuDH) and glucose dehydrogenase (GDH) has showed great coupling catalytic efficiency in the synthesis of l-tle, however the multi-enzyme complex of GDH and LeuDH has never been constructed successfully. Results In this work, a novel fusion enzyme (GDH–R3–LeuDH) for the efficient biosynthesis of l-tle was constructed by the fusion of LeuDH and GDH mediated with a rigid peptide linker. Compared with the free enzymes, both the environmental tolerance and thermal stability of GDH–R3–LeuDH had a great improved since the fusion structure. The fusion structure also accelerated the cofactor regeneration rate and maintained the enzyme activity, so the productivity and yield of l-tle by GDH–R3–LeuDH was all enhanced by twofold. Finally, the space–time yield of l-tle catalyzing by GDH–R3–LeuDH whole cells could achieve 2136 g/L/day in a 200 mL scale system under the optimal catalysis conditions (pH 9.0, 30 °C, 0.4 mM of NAD+ and 500 mM of a substrate including trimethylpyruvic acid and glucose). Conclusions It is the first report about the fusion of GDH and LeuDH as the multi-enzyme complex to synthesize l-tle and reach the highest space–time yield up to now. These results demonstrated the great potential of the GDH–R3–LeuDH fusion enzyme for the efficient biosynthesis of l-tle.

Instandhalter sind Mitgestalter ihres Assistenzsystems/Maintenance engineers are co-designers of their assistance system

2019 ◽  
Vol 109 (03) ◽  
pp. 189-193
Author(s):  
A. Keller ◽  
S. Adler ◽  
N. Zobel
Keyword(s):  
Technological Development ◽  
Assistance System ◽  
User Requirements ◽  
Shift Workers ◽  
System A ◽  
Work In Process ◽  
Kleine Und Mittlere Unternehmen ◽  
System P ◽  
Process Manufacturing ◽  
Maintenance Work

Im Forschungsprojekt „CPPSProcessAssist“ wurde für vier kleine und mittlere Unternehmen (KMU) der Prozessindustrie ein modulares und mobiles Assistenzsystem zur Instandhaltungsunterstützung entwickelt. Neben der technologischen Entwicklung des Systems wurde im Projekt eine Vorgehensweise zur Gestaltung und Einführung des Systems in den Unternehmen erarbeitet. Mitarbeiter der Schicht und Schichtleitung, Projektingenieure, Instandhalter sowie Technologieentwickler wurden als Mitgestalter des Systems und der daraus folgenden Veränderungen im Unternehmen eingebunden. Das resultierende System bildet die unternehmensübergreifenden Anforderungen von Instandhaltungsaufgaben in der Prozessindustrie technisch ab und ist an vorhandene IT-Infrastrukturen anpassbar. Die gewählte Vorgehensweise zur Einbindung der Mitarbeiter in der Gestaltung des Systems trug dazu bei, die unternehmens- und nutzerspezifischen Anforderungen in das System zu integrieren sowie die Mitarbeiter zu Eingabe bedarfsgerechter Assistenzinhalten zu befähigen.   A modular and portable maintenance assistance system was developed for four small and medium-sized process manufacturers in the research project CPPSProcessAssist. Along with the technological development of the system, a procedure for designing and implementing the system in the companies was developed. Shift workers and managers, project engineers, maintenance engineers and technology developers were involved in designing the system and implementing the ensuing changes in their companies. The resulting system technically maps cross-company requirements for maintenance work in process manufacturing and can be modified for existing IT infrastructures. The selected procedure for involving employees in the system design helped integrating specific company and user requirements in the system and enabling employees to enter content in the assistance system based on their needs.

scholarly journals Mahindra First Choice

2021 ◽  
pp. 354-362
Author(s):  
Ben Jackson ◽  
Genevieve Joy
Keyword(s):  
Third Party ◽  
Repair System ◽  
Market Failures ◽  
System A ◽  
Car Market ◽  
System P ◽  
First Choice ◽  
Used Car ◽  
The Creation ◽  
Trust Information

Mahindra Firstchoice illustrates the process of ecosystem orchestration in the context of the second-hand car market in India. It describes how Mahindra Firstchoice mapped the ecosystem in relation to six key parties—consumers who were buyers, consumers who were sellers, car manufacturers, independent used-car dealers, independent car service workshops, and banks. It then identified the bottlenecks and ‘pain points’ that afflicted the six parties. The used-car market did not function properly because of lack of trust, information, and transparency and Mahindra Firstchoice worked with the parties to identify solutions to the market failures. These involved, amongst other things, the creation of third-party car inspection services, the establishment of a multi-brand car-dealer franchise, a warranty system, a bluebook of second-hand prices and transactions, and a car diagnosis and repair system.

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