scholarly journals Demethylation of vanillic acid by recombinant LigM in a one-pot cofactor regeneration system

2016 ◽  
Vol 6 (21) ◽  
pp. 7729-7737 ◽  
Author(s):  
Elena Rosini ◽  
Paola D'Arrigo ◽  
Loredano Pollegioni
Keyword(s):  
Vanillic Acid ◽  
Cofactor Regeneration ◽  
Methionine Synthase ◽  
Regeneration System ◽  
One Pot ◽  
Cofactor Recycling ◽  
System P

Recombinant LigM from Sphingobium SYK-6 and plant methionine synthase MetE enzyme efficiently convert vanillic acid into PCA with cofactor recycling.

Efficient asymmetric synthesis of chiral alcohols using high 2-propanol tolerance alcohol dehydrogenase SmADH2 via an environmentally friendly TBCR system

2020 ◽  
Vol 10 (1) ◽  
pp. 70-78 ◽  
Author(s):  
Zeyu Yang ◽  
Hengwei Fu ◽  
Wenjie Ye ◽  
Youyu Xie ◽  
Qinghai Liu ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Asymmetric Synthesis ◽  
Environmentally Friendly ◽  
Cofactor Regeneration ◽  
Chiral Alcohols ◽  
Regeneration System ◽  
High Substrate ◽  
System A ◽  
System P

Based on substrate-coupled cofactor regeneration system, a high 2-propanol tolerance SmADH2 together with TBCR system can synthesise structurally diverse chiral alcohols at a high substrate loading with only 1.25 equivalents of 2-propanol.

Cell-Free Total Biosynthesis of Plant Terpene Natural Products Using an Orthogonal Cofactor Regeneration System

ACS Catalysis ◽  
2021 ◽  
pp. 9898-9903
Author(s):  
Undramaa Bat-Erdene ◽  
John M. Billingsley ◽  
William C. Turner ◽  
Benjamin R. Lichman ◽  
Francesca M. Ippoliti ◽  
...  
Keyword(s):  
Natural Products ◽  
Cofactor Regeneration ◽  
Regeneration System

scholarly journals Construction and characterization of a novel glucose dehydrogenase-leucine dehydrogenase fusion enzyme for the biosynthesis of l-tert-leucine

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Langxing Liao ◽  
Yonghui Zhang ◽  
Yali Wang ◽  
Yousi Fu ◽  
Aihui Zhang ◽  
...  
Keyword(s):  
Glucose Dehydrogenase ◽  
Catalytic Efficiency ◽  
Space Time ◽  
Cofactor Regeneration ◽  
Enzyme Complex ◽  
Regeneration System ◽  
Regeneration Rate ◽  
Leucine Dehydrogenase ◽  
Fusion Enzyme ◽  
Space Time Yield

Abstract Background Biosynthesis of l-tert-leucine (l-tle), a significant pharmaceutical intermediate, by a cofactor regeneration system friendly and efficiently is a worthful goal all the time. The cofactor regeneration system of leucine dehydrogenase (LeuDH) and glucose dehydrogenase (GDH) has showed great coupling catalytic efficiency in the synthesis of l-tle, however the multi-enzyme complex of GDH and LeuDH has never been constructed successfully. Results In this work, a novel fusion enzyme (GDH–R3–LeuDH) for the efficient biosynthesis of l-tle was constructed by the fusion of LeuDH and GDH mediated with a rigid peptide linker. Compared with the free enzymes, both the environmental tolerance and thermal stability of GDH–R3–LeuDH had a great improved since the fusion structure. The fusion structure also accelerated the cofactor regeneration rate and maintained the enzyme activity, so the productivity and yield of l-tle by GDH–R3–LeuDH was all enhanced by twofold. Finally, the space–time yield of l-tle catalyzing by GDH–R3–LeuDH whole cells could achieve 2136 g/L/day in a 200 mL scale system under the optimal catalysis conditions (pH 9.0, 30 °C, 0.4 mM of NAD+ and 500 mM of a substrate including trimethylpyruvic acid and glucose). Conclusions It is the first report about the fusion of GDH and LeuDH as the multi-enzyme complex to synthesize l-tle and reach the highest space–time yield up to now. These results demonstrated the great potential of the GDH–R3–LeuDH fusion enzyme for the efficient biosynthesis of l-tle.

Oxidation-responsive micelles by a one-pot polymerization-induced self-assembly approach

2018 ◽  
Vol 9 (13) ◽  
pp. 1593-1602 ◽  
Author(s):  
Fabian H. Sobotta ◽  
Franziska Hausig ◽  
Dominic O. Harz ◽  
Stephanie Hoeppener ◽  
Ulrich S. Schubert ◽  
...  
Keyword(s):  
Self Assembly ◽  
Raft Polymerization ◽  
Assembly Process ◽  
Carrier System ◽  
One Pot ◽  
System P

Combining a sequential, one-pot RAFT polymerization with the polymerization-induced self-assembly process results in a versatile oxidation-responsive carrier system.

One-pot synthesis of 3-aminofurans using a simple and efficient recyclable CuI/[bmim]PF6 system

2021 ◽  
Author(s):  
Guguloth Veeranna ◽  
Ramesh Balaboina ◽  
Narasimha Swamy Thirukovela ◽  
Ravindhar Vadde
Keyword(s):  
Secondary Amines ◽  
Terminal Alkynes ◽  
One Pot ◽  
One Pot Synthesis ◽  
System P ◽  
Three Component Reaction ◽  
The One

The one-pot three-component reaction of several 2-ketoaldehdyes, secondary amines and terminal alkynes to access 3-aminofurans was proceeded well in [bmim][PF6] using a simple and cheap CuI catalyst. The resulted 3-aminofuran...

A practical green approach to diversified spirochromene/spiropyran scaffolds via a glucose–water synergy driven organocatalytic system

2018 ◽  
Vol 42 (21) ◽  
pp. 17279-17290 ◽  
Author(s):  
Fatima Tufail ◽  
Mohammad Saquib ◽  
Swastika Singh ◽  
Jyoti Tiwari ◽  
Preety Dixit ◽  
...  
Keyword(s):  
Green Method ◽  
One Pot ◽  
Green Approach ◽  
System P ◽  
Glucose Water

Development of a versatile, new glucose–water organocatalytic system promoted, one pot, practical green method for the synthesis of diversified spirochromenes/spiropyrans is reported.

Round, round we go – strategies for enzymatic cofactor regeneration

2020 ◽  
Vol 37 (10) ◽  
pp. 1316-1333 ◽  
Author(s):  
Silja Mordhorst ◽  
Jennifer N. Andexer
Keyword(s):  
Cofactor Regeneration ◽  
Regeneration System ◽  
Complex Molecules

Combined with an efficient and flexible regeneration system, cofactor-dependent enzymes can be used to selectively introduce modifications in complex molecules.

One-pot synthesis of bimetallic metal–organic frameworks (MOFs) as acid–base bifunctional catalysts for tandem reaction

2020 ◽  
Vol 10 (2) ◽  
pp. 315-322 ◽  
Author(s):  
Yanjing Hu ◽  
Jian Zhang ◽  
Hang Huo ◽  
Zhe Wang ◽  
Xianzhu Xu ◽  
...  
Keyword(s):  
Metal Organic Frameworks ◽  
Bifunctional Catalysts ◽  
Pore System ◽  
Acid Base ◽  
One Pot ◽  
System P ◽  
Synergistic Mechanism ◽  
Metal Organic ◽  
Hierarchical Pore System ◽  
Hierarchical Pore

Bimetallic MIL-101(Al/Fe)–NH2 exhibits enhanced acid–base bifunctional catalytic activity due to its synergistic mechanism and hierarchical pore system.

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