Delayed vibrational modulation of the solvated GFP chromophore into a conical intersection

2019 ◽  
Vol 21 (19) ◽  
pp. 9728-9739 ◽  
Author(s):  
Miles A. Taylor ◽  
Liangdong Zhu ◽  
Nikita D. Rozanov ◽  
Kenneth T. Stout ◽  
Cheng Chen ◽  
...  
Keyword(s):  
Conical Intersection ◽  
Gfp Chromophore

Upon photoexcitation, a delayed structural bending motion facilitates the “dark” GFP chromophore ring-twisting isomerization in reaching a conical intersection in solution.

scholarly journals Internal conversion of the anionic GFP chromophore: in and out of the I-twisted S1/S0 conical intersection seam

2022 ◽  
Author(s):  
Nanna H. List ◽  
Chey M. Jones ◽  
Todd J. Martínez
Keyword(s):  
Quantum Yield ◽  
Internal Conversion ◽  
Conical Intersection ◽  
Statistical Dynamics ◽  
Statistical Behavior ◽  
Gfp Chromophore

The Z–E photoisomerization quantum yield of the HBDI− chromophore is a result of early, non-statistical dynamics around a less reactive I-twisted intersection and later, statistical behavior around the more reactive, near-enantiomeric counterpart.

Structural Evidence of Photoisomerization Pathways in Fluorescent Proteins

2019 ◽  
Author(s):  
Jeffrey Chang ◽  
Matthew Romei ◽  
Steven Boxer
Keyword(s):  
Rational Design ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Super Resolution ◽  
Unit Cell Size ◽  
Chlorine Substituent ◽  
Gfp Chromophore ◽  
The One ◽  
Green Fluorescent ◽  
Super Resolution Microscopy

<p>Double-bond photoisomerization in molecules such as the green fluorescent protein (GFP) chromophore can occur either via a volume-demanding one-bond-flip pathway or via a volume-conserving hula-twist pathway. Understanding the factors that determine the pathway of photoisomerization would inform the rational design of photoswitchable GFPs as improved tools for super-resolution microscopy. In this communication, we reveal the photoisomerization pathway of a photoswitchable GFP, rsEGFP2, by solving crystal structures of <i>cis</i> and <i>trans</i> rsEGFP2 containing a monochlorinated chromophore. The position of the chlorine substituent in the <i>trans</i> state breaks the symmetry of the phenolate ring of the chromophore and allows us to distinguish the two pathways. Surprisingly, we find that the pathway depends on the arrangement of protein monomers within the crystal lattice: in a looser packing, the one-bond-flip occurs, whereas in a tighter packing (7% smaller unit cell size), the hula-twist occurs.</p><p> </p><p> </p><p> </p><p> </p><p> </p><p> </p> <p> </p>

scholarly journals Enhancement of strong-field multiple ionization in the vicinity of the conical intersection in 1,3-cyclohexadiene ring opening

2013 ◽  
Vol 139 (18) ◽  
pp. 184309 ◽  
Author(s):  
Vladimir S. Petrovic ◽  
Sebastian Schorb ◽  
Jaehee Kim ◽  
James White ◽  
James P. Cryan ◽  
...  
Keyword(s):  
Ring Opening ◽  
Strong Field ◽  
Conical Intersection ◽  
Multiple Ionization

Dual-Self-Restricted GFP Chromophore Analogues with Significantly Enhanced Emission

2020 ◽  
Vol 124 (5) ◽  
pp. 871-880 ◽  
Author(s):  
Hongping Deng ◽  
Chunyang Yu ◽  
Deyue Yan ◽  
Xinyuan Zhu
Keyword(s):  
Gfp Chromophore

The Case of the Missing Ring:  Radical Cleavage of a Carbon−Carbon Bond and Implications for GFP Chromophore Biosynthesis

2007 ◽  
Vol 129 (11) ◽  
pp. 3118-3126 ◽  
Author(s):  
David P. Barondeau ◽  
Carey J. Kassmann ◽  
John A. Tainer ◽  
Elizabeth D. Getzoff
Keyword(s):  
Carbon Bond ◽  
Carbon Carbon Bond ◽  
Gfp Chromophore ◽  
Radical Cleavage ◽  
Missing Ring

Wave Packet Dynamics in the Presence of a Conical Intersection

1997 ◽  
Vol 101 (19) ◽  
pp. 3454-3460 ◽  
Author(s):  
Paola Cattaneo ◽  
Maurizio Persico
Keyword(s):  
Wave Packet ◽  
Conical Intersection ◽  
Wave Packet Dynamics
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