Reversible detection of phosphorylation and dephosphorylation by tip-enhanced Raman spectroscopy using a cyclopentadienyl ruthenium nanotag functionalized tip

2020 ◽  
Vol 56 (6) ◽  
pp. 936-939 ◽  
Author(s):  
Yi-Cheng Lin ◽  
Zi-Yu Ke ◽  
Pei-Hsuan Liao ◽  
Ching-Yu Tseng ◽  
Kien Voon Kong
Keyword(s):  
Raman Spectroscopy ◽  
Cell Signaling ◽  
Reversible Phosphorylation ◽  
Sensing System ◽  
A Cell ◽  
Tip Enhanced Raman Spectroscopy

We employed a TERS tip and SERS nanotags to create a cell signaling based sensing system that is capable of creating a reversible phosphorylation/de-phosphorylation cycle for TERS measurement.

Surface-and tip-enhanced Raman spectroscopy of silicon

2008 ◽  
Author(s):  
K. J. Yi ◽  
X. N. He ◽  
W. Q. Yang ◽  
Y. S. Zhou ◽  
W. Xiong ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Tip Enhanced Raman Spectroscopy

Protein Flexibility Catalyzes a Cell Signaling Reaction of the Ras-GAP Complex

2019 ◽  
Author(s):  
Keiei Kumon ◽  
Masahiro Higashi ◽  
Shinji Saito ◽  
Shigehiko Hayashi
Keyword(s):  
Cell Signaling ◽  
Conformational Changes ◽  
Catalytic Reaction ◽  
Protein Complex ◽  
Enzyme Mechanism ◽  
Activation Free Energy ◽  
Chemical Catalysis ◽  
Simple Chemical ◽  
A Cell ◽  
Enzyme Molecules

Many enzyme molecules exhibit characteristic global and slow dynamics which furnish them with allostery realizing remarkable molecular functionalities more than simple chemical catalysis. However, molecular mechanism of a catalytic reaction associated with the molecular flexibility of enzymes is not well-understood. Here we report a hybrid molecular simulation study on GTPase activity of a Ras-GAP protein complex for cell signaling termination. We unveiled that extensive conformational changes of the protein complex and exclusion of internal water molecules are induced upon the transition state (TS) formation in the catalytic reaction and significantly lower the reaction activation free energy. We also revealed that tumor-related mutations perturb those conformational changes upon the TS formation, leading to reduction of the catalytic activity. The findings of the remarkably dynamic protein conformation directly linking to the catalytic reaction have broad implications for understanding of enzyme mechanism and for developments of allosteric drugs and novel catalysts.

Capability of tip-enhanced Raman spectroscopy about nanoscale analysis of strained silicon for semiconductor devices production

2017 ◽  
Vol 110 (10) ◽  
pp. 103105 ◽  
Author(s):  
Arianna Lucia ◽  
Onofrio Antonino Cacioppo ◽  
Enrico Iulianella ◽  
Luca Latessa ◽  
Giuseppe Moccia ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Semiconductor Devices ◽  
Strained Silicon ◽  
Tip Enhanced Raman Spectroscopy

Recent Advances in Tip-Enhanced Raman Spectroscopy

2014 ◽  
Vol 5 (18) ◽  
pp. 3125-3130 ◽  
Author(s):  
Matthew D. Sonntag ◽  
Eric A. Pozzi ◽  
Nan Jiang ◽  
Mark C. Hersam ◽  
Richard P. Van Duyne
Keyword(s):  
Raman Spectroscopy ◽  
Recent Advances ◽  
Tip Enhanced Raman Spectroscopy

scholarly journals Fabrication of highly uniform nanoprobe via the automated process for tip-enhanced Raman spectroscopy

Nanophotonics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 2989-2996
Author(s):  
Dong Hyeon Kim ◽  
Chanwoo Lee ◽  
Byeong Geun Jeong ◽  
Sung Hyuk Kim ◽  
Mun Seok Jeong
Keyword(s):  
Raman Spectroscopy ◽  
Human Error ◽  
Acidic Solution ◽  
High Spatial Resolution ◽  
Electrochemical Etching ◽  
Radius Of Curvature ◽  
Enhanced Raman Scattering ◽  
Tip Enhanced Raman Spectroscopy ◽  
Highly Uniform ◽  
Etching System

AbstractIn a tip-enhanced Raman spectroscopy (TERS) system, using a sharp nanotip that comprises a noble metal is critical to attaining high spatial resolution and highly enhanced Raman scattering. A strongly acidic solution is typically used to fabricate gold nanotips in a quick and reliable manner. However, using an acidic solution could corrode the etching system, thereby posing hazardous problems. Therefore, both the corrosion of the etching system and human error induced by the conventional method considerably decrease the quality and reproducibility of the tip. In this study, we significantly increased the reproducibility of tip fabrication by automating the electrochemical etching system. In addition, we optimized the etching conditions for an etchant that comprised a KCl solution to which ethanol was added to overcome the limitations of the acidic etchant. The automated etching system significantly increases the yield rate of tip-fabrication reproducibility from 65 to 95%. The standard deviation of the radius of curvature decreased to 7.3 nm with an average radius of curvature of 30 nm. Accordingly, the automated electrochemical etching system might improve the efficiency of TERS.

Sign in / Sign up

Export Citation Format

Share Document