Target-triggered, self-powered DNAzyme–MnO2 nanosystem: towards imaging microRNAs in living cells

2019 ◽  
Vol 55 (89) ◽  
pp. 13366-13369 ◽  
Author(s):  
Mei-Rong Cui ◽  
Xiang-Ling Li ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Signal Amplification ◽  
Living Cells ◽  
Intracellular Signal ◽  
Self Powered

We constructed a versatile self-powered DNAzyme–MnO2 nanosystem for intracellular signal amplification and sensitive imaging of miRNAs in living cells.

A self-powered 3D DNA walker with programmability and signal-amplification for illuminating microRNA in living cells

2020 ◽  
Vol 56 (14) ◽  
pp. 2135-2138 ◽  
Author(s):  
Xue-Jiao Yang ◽  
Mei-Rong Cui ◽  
Xiang-Ling Li ◽  
Hong-Yuan Chen ◽  
Jing-Juan Xu
Keyword(s):  
Signal Amplification ◽  
Living Cells ◽  
Imaging Analysis ◽  
Dna Walker ◽  
Intracellular Signal ◽  
Self Powered

We construct a target-triggered, self-powered 3D DNA walker for achieving intracellular signal amplification and sensitive imaging analysis of microRNAs.

A high-energy sandwich-type self-powered biosensor based on DNA bioconjugates and a nitrogen doped ultra-thin carbon shell

2020 ◽  
Vol 8 (7) ◽  
pp. 1389-1395 ◽  
Author(s):  
Fu-Ting Wang ◽  
Yi-Han Wang ◽  
Jing Xu ◽  
Ke-Jing Huang
Keyword(s):  
Signal Amplification ◽  
High Energy ◽  
Biofuel Cells ◽  
Ultrasensitive Detection ◽  
Nitrogen Doped ◽  
Sensing Platform ◽  
Enzymatic Biofuel Cells ◽  
Thin Carbon ◽  
Sandwich Type ◽  
Self Powered

A high-energy self-powered sensing platform for the ultrasensitive detection of proteins is developed based on enzymatic biofuel cells (EBFCs) by using DNA bioconjugate assisted signal amplification.

Self-Assembly Trigger Signal Amplification for MicroRNA Sensing in Living Cells with GSH-Cleavable Nanoprobes

2020 ◽  
Vol 59 (47) ◽  
pp. 20582-20590
Author(s):  
Liman Xian ◽  
Haoying Ge ◽  
Ning Xu ◽  
Feng Xu ◽  
Qichao Yao ◽  
...  
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Living Cells ◽  
Trigger Signal

scholarly journals Imaging multiple microRNAs in living cells using ATP self-powered strand-displacement cascade amplification

2018 ◽  
Vol 9 (5) ◽  
pp. 1184-1190 ◽  
Author(s):  
Xiangdan Meng ◽  
Wenhao Dai ◽  
Kai Zhang ◽  
Haifeng Dong ◽  
Xueji Zhang
Keyword(s):  
Living Cells ◽  
Strand Displacement ◽  
Displacement Cascade ◽  
Highly Sensitive ◽  
Mirna Detection ◽  
Self Powered ◽  
Cascade Amplification

Herein, we design a smart autonomous ATP self-powered strand displacement cascade amplification (SDCA) system for highly sensitive multiple intracellular miRNA detection.

scholarly journals Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells

2021 ◽  
Author(s):  
Yang Liu ◽  
Ning Zhao ◽  
Masato T Kanemaki ◽  
Yotaro Yamamoto ◽  
Yoshifusa Sadamura ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Signal Amplification ◽  
Fluorescent Proteins ◽  
Close Association ◽  
Zinc Finger Proteins ◽  
Living Cells ◽  
Regulate Gene Expression ◽  
Cohesin Subunit ◽  
Single Living Cells ◽  
Single Living

In eukaryotic nuclei, chromatin loops mediated through cohesin are critical structures that regulate gene expression and DNA replication. Here we demonstrate a new method to visualize endogenous genomic loci using synthetic zinc-finger proteins harboring repeat epitope tags (ZF probes) for signal amplification via binding of tag-specific intracellular antibodies, or frankenbodies, fused with fluorescent proteins. We achieve this in two steps. First, we develop an anti-FLAG frankenbody that can bind FLAG-tagged proteins in diverse live-cell environments. The anti-FLAG frankenbody complements the anti-HA frankenbody, enabling two-color signal amplification from FLAG and HA-tagged proteins. Second, we develop a pair of cell-permeable ZF probes that specifically bind two endogenous chromatin loci predicted to be involved in chromatin looping. By coupling our anti-FLAG and anti-HA frankenbodies with FLAG- and HA-tagged ZF probes, we simultaneously visualize the dynamics of the two loci in single living cells. This reveals close association between the two loci in the majority of cells, but the loci markedly separate upon the triggered degradation of the cohesin subunit RAD21. Our ability to image two endogenous genomic loci simultaneously in single living cells provides a proof-of-principle that ZF probes coupled with frankenbodies are useful new tools for exploring genome dynamics in multiple colors.

A microRNA-triggered self-powered DNAzyme walker operating in living cells

2019 ◽  
Vol 136 ◽  
pp. 31-37 ◽  
Author(s):  
Chang Liu ◽  
Yanlei Hu ◽  
Qingshan Pan ◽  
Jintao Yi ◽  
Juan Zhang ◽  
...  
Keyword(s):  
Living Cells ◽  
Self Powered

Monitoring VEGF mRNA and imaging in living cells in vitro using rGO-based dual fluorescent signal amplification platform

Talanta ◽  
2019 ◽  
Vol 205 ◽  
pp. 120092
Author(s):  
Wenya Dang ◽  
Hao Liu ◽  
Jialong Fan ◽  
Chuan Zhao ◽  
Ying Long ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Living Cells ◽  
Vegf Mrna ◽  
Fluorescent Signal

scholarly journals S2L1 Single molecule analysis of intracellular signal transduction in living cells

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S11
Author(s):  
T. Kobayashi ◽  
M. Murakami ◽  
T. Kawasaki ◽  
A. Yoshimura ◽  
A. Kusumi
Keyword(s):  
Signal Transduction ◽  
Single Molecule ◽  
Living Cells ◽  
Intracellular Signal Transduction ◽  
Intracellular Signal ◽  
Single Molecule Analysis
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