Rolling “wool-balls”: rapid live-cell mapping of membrane sialic acids via poly-p-benzoquinone/ethylenediamine nanoclusters

2019 ◽  
Vol 55 (65) ◽  
pp. 9681-9684 ◽  
Author(s):  
Bin-Bin Chen ◽  
Xiao-Yuan Wang ◽  
Ruo-Can Qian
Keyword(s):  
Cell Surface ◽  
Sialic Acids ◽  
Live Cell ◽  
Cell Mapping

In this work, we design sticky, furry and fluorescent “wool-balls” based on p-benzoquinone/ethylenediamine polymer nanoclusters, which provide a convenient, fast labeling strategy for the imaging of cell surface sialic acids.

scholarly journals Cell Surface Receptors: Rapid Quantification of Live Cell Receptors Using Bioluminescence in a Flow-Based Microfluidic Device (Small 8/2015)

Small ◽  
2015 ◽  
Vol 11 (8) ◽  
pp. 1012-1012
Author(s):  
Ramesh Ramji ◽  
Cheong Fook Cheong ◽  
Hiroaki Hirata ◽  
Abdur Rub Abdur Rahman ◽  
Chwee Teck Lim
Keyword(s):  
Cell Surface ◽  
Microfluidic Device ◽  
Live Cell ◽  
Cell Surface Receptors ◽  
Cell Receptors ◽  
Surface Receptors ◽  
Rapid Quantification

Identification of sialic acids on the cell surface of hyphae and conidia of the human pathogenFonsecaea pedrosoi

1986 ◽  
Vol 24 (2) ◽  
pp. 145-154 ◽  
Author(s):  
E.T. Souza ◽  
F.C. Silva-Filho ◽  
W. De Souza ◽  
C.S. Alviano ◽  
J. Angluster ◽  
...  
Keyword(s):  
Cell Surface ◽  
Sialic Acids

Metabolic expression of thiol-derivatized sialic acids on the cell surface and their quantitative estimation by flow cytometry

2006 ◽  
Vol 1 (4) ◽  
pp. 1840-1851 ◽  
Author(s):  
Srinivasa-Gopalan Sampathkumar ◽  
Mark B Jones ◽  
Kevin J Yarema
Keyword(s):  
Flow Cytometry ◽  
Cell Surface ◽  
Quantitative Estimation ◽  
Sialic Acids

Generation of Live-Cell Microarrays by Means of DNA-Directed Immobilization of Specific Cell-Surface Ligands

2007 ◽  
Vol 46 (22) ◽  
pp. 4180-4183 ◽  
Author(s):  
Hendrik Schroeder ◽  
Bernhard Ellinger ◽  
Christian F. W. Becker ◽  
Herbert Waldmann ◽  
Christof M. Niemeyer
Keyword(s):  
Cell Surface ◽  
Live Cell ◽  
Specific Cell ◽  
Surface Ligands ◽  
Cell Microarrays ◽  
Specific Cell Surface

Accurate quantitative detection of cell surface sialic acids with a background-free SERS probe

Talanta ◽  
2020 ◽  
Vol 209 ◽  
pp. 120579 ◽  
Author(s):  
Xiang-Nan He ◽  
Ya-Ning Wang ◽  
Yue Wang ◽  
Zhang-Run Xu
Keyword(s):  
Cell Surface ◽  
Sialic Acids ◽  
Quantitative Detection

scholarly journals Use of Novel Mutant Galactosyltransferase for the Bioconjugation of Terminal N-Acetylglucosamine (GlcNAc) Residues on Live Cell Surface

2013 ◽  
Vol 24 (1) ◽  
pp. 144-152 ◽  
Author(s):  
Natalia Mercer ◽  
Boopathy Ramakrishnan ◽  
Elizabeth Boeggeman ◽  
Luke Verdi ◽  
Pradman K. Qasba
Keyword(s):  
Cell Surface ◽  
Live Cell

scholarly journals A STUDY ON THE MECHANISM OF INTERCELLULAR ADHESION

1974 ◽  
Vol 60 (3) ◽  
pp. 641-652 ◽  
Author(s):  
Joris J. Deman ◽  
Erik A. Bruyneel ◽  
Marc M. Mareel
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Calcium Ions ◽  
Hela Cells ◽  
Previous Treatment ◽  
Cell Aggregation ◽  
Sialic Acids ◽  
Magnesium Ions ◽  
Trypsin Treatment ◽  
Calcium Effect

Aggregation of suspended HeLa cells is increased on removal of cell surface sialic acid. Calcium ions promote aggregation whereas magnesium ions have no effect. The calcium effect is abolished by previous treatment of the cells with neuraminidase. Trypsinization of the HeLa cells followed by thorough washing diminishes the rate of mutual cell aggregation. Subsequent incubation with neuraminidase restores the aggregation rate to the original value before trypsin treatment. Cells which had acquired a greater tendency for aggregation after removal of peripheral sialic acid lose this property when subsequently treated with trypsin. Calcium ions have no aggregative effect on trypsinized cells. In contrast to HeLa cells, aggregation of human erythrocytes was not increased after treatment with neuraminidase or on addition of calcium. The results with HeLa cells are interpreted as follows: (a) Trypsin-releasable material confers adhesiveness upon the cells. (b) The adhesive property of this material is counteracted by the presence of cell surface sialic acids. (c) Calcium ions exert their effect by attenuating the adverse effect of sialic acid.

scholarly journals Golgi, trafficking, and mitosis dysfunctions in pulmonary arterial endothelial cells exposed to monocrotaline pyrrole and NO scavenging

2009 ◽  
Vol 297 (4) ◽  
pp. L715-L728 ◽  
Author(s):  
Jason Lee ◽  
Reuben Reich ◽  
Fang Xu ◽  
Pravin B. Sehgal
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Cell Surface ◽  
Live Cell ◽  
Cell Uptake ◽  
Important Distinction ◽  
No Donor ◽  
Pulmonary Arterial ◽  
Monocrotaline Pyrrole ◽  
Arterial Endothelial Cells

Although the administration of monocrotaline (MCT) into experimental animals is in widespread use today in investigations of pulmonary arterial hypertension (PAH), the underlying cellular and subcellular mechanisms that culminate in vascular remodeling are incompletely understood. Bovine pulmonary arterial endothelial cells (PAECs) in culture exposed to monocrotaline pyrrole (MCTP) develop “megalocytosis” 18–24 h later characterized by enlarged hyperploid cells with enlarged Golgi, mislocalization of endothelial nitric oxide synthase away from the plasma membrane, decreased cell-surface/caveolar nitric oxide (NO), and hypo- S-nitrosylation of caveolin-1, clathrin heavy chain, and N-ethylmaleimide-sensitive factor. We investigated whether MCTP did in fact affect functional intracellular trafficking. The NO scavenger (4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) and the NO donor diethylamine NONOate were used for comparison. Both MCTP and c-PTIO produced distinctive four- to fivefold enlarged PAECs within 24–48 h with markedly enlarged/dispersed Golgi, as visualized by immunostaining for the Golgi tethers/matrix proteins giantin, GM130, and p115. Live-cell uptake of the Golgi marker C5 ceramide revealed a compact juxtanuclear Golgi in untreated PAECs, brightly labeled enlarged circumnuclear Golgi after MCTP, but minimally labeled Golgi elements after c-PTIO. These Golgi changes were reduced by NONOate. After an initial inhibition during the first day, both MCTP and c-PTIO markedly enhanced anterograde secretion of soluble cargo (exogenous vector-expressed recombinant horseradish peroxidase) over the next 4 days. Live-cell internalization assays using fluorescently tagged ligands showed that both MCTP and c-PTIO inhibited the retrograde uptake of acetylated low-density lipoprotein, transferrin, and cholera toxin B. Moreover, MCTP, and to a variable extent c-PTIO, reduced the cell-surface density of all receptors assayed (LDLR, TfnR, BMPR, Tie-2, and PECAM-1/CD31). In an important distinction, c-PTIO enhanced mitosis in PAECs but MCTP inhibited mitosis, even that due to c-PTIO, despite markedly exaggerated Golgi dispersal. Taken together, these data define a broad-spectrum Golgi and subcellular trafficking dysfunction syndrome in endothelial cells exposed to MCTP or NO scavenging.

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