Ultrasensitive detection of long non-coding RNAs based on duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification

2019 ◽  
Vol 55 (48) ◽  
pp. 6827-6830 ◽  
Author(s):  
Yan Zhang ◽  
Xin-yan Wang ◽  
Xianwei Su ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
New Method ◽  
Sensitive Detection ◽  
Ultrasensitive Detection ◽  
Non Coding Rnas

We develop a new method for sensitive detection of long noncoding RNAs using duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification.

Integration of a peptide–DNA conjugate with multiple cyclic signal amplification for the ultrasensitive detection of cathepsin B activity

2020 ◽  
Vol 56 (14) ◽  
pp. 2119-2122 ◽  
Author(s):  
Zi-yue Wang ◽  
Cheng-peng Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Cathepsin B ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Ultrasensitive Detection

We integrate a peptide–DNA conjugate with multiple cyclic signal amplification for the sensitive detection of cathepsin B activity.

scholarly journals A new cancer-testis long noncoding RNA, the OTP-AS1 RNA

2018 ◽  
Author(s):  
Iuliia K. Karnaukhova ◽  
Dmitrii E. Polev ◽  
Larisa L. Krukovskaya ◽  
Alexey E. Masharsky ◽  
Olga V. Nazarenko ◽  
...  
Keyword(s):  
Long Noncoding Rna ◽  
Noncoding Rna ◽  
Regulation Of Gene Expression ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Protein Coding ◽  
Regulatory Interactions ◽  
Normal Tissues ◽  
Non Coding Rnas ◽  
Cancer Testis

AbstractOrthopedia homeobox (OTP) gene encodes a homeodomain-containing transcription factor involved in brain development. OTP is mapped to human chromosome 5q14.1. Earlier we described transcription in the second intron of this gene in wide variety of tumors, but among normal tissues only in testis. In GeneBank these transcripts are presented by several 300-400 nucleotides long AI267901-like ESTs.We assumed that AI267901-like ESTs belong to longer transcript(s). We used the Rapid Amplification of cDNA Ends (RACE) approach and other methods to find the full-length transcript. The found transcript was 2436 nucleotides long polyadenylated sequence in antisense to OTP gene. The corresponding gene consisted of two exons separated by an intron of 2961 bp long. The first exon was found to be 91 bp long and located in the third exon of OTP gene. The second exon was 2345bp long and located in the second intron of OTP gene.The search of possible open reading frames (ORFs) showed the lack of significant ORFs. We have shown the expression of new gene in many human tumors and only in one sampled normal testis. The data suggest that we discovered a new antisense cancer-testis sequence OTP-AS1 (OTP- antisense RNA 1), which belongs to long noncoding RNAs (lncRNAs). According to our findings we assume that OTP-AS1 and OTP genes may be the CT-coding gene/CT-ncRNA pair involved in regulatory interactions.Author summaryPreviously, long non-coding RNAs (lncRNAs) were considered as genetic “noise”. However, it was later shown that only 2% of genomic transcripts have a protein-coding ability. Non-coding RNA is divided into short non-coding RNAs (20-200 nucleotides) and long noncoding RNAs (200-100,000 nucleotides). Genes encoding lncRNA often overlap or are adjacent to protein-coding genes, and localization of this kind is beneficial in order to regulate the transcription of neighboring genes. Studies have shown that of lncRNAs play many roles in the regulation of gene expression. New evidence indicates that dysfunctions of lncRNAs are associated with human diseases and cancer.In our study we found a new cancer-testis long noncoding RNA (OTP-AS1), which is an antisense of protein-coding cancer-testis gene (OTP). Thus, OTP-AS1 and OTP genes may be the CT-coding gene/CT-ncRNA pair involved in regulatory interactions. This is supported by the similar profile of their expression. OTP-AS1 may be of interest as a potential diagnostic marker of cancer or a potential target for cancer therapy.Part of OTP-AS1 gene (5’-end of the second exon) is evolutionary younger than the rest of gene sequence and is less conservative. This links OTP-AS1 gene with so-called TSEEN (tumor-specifically expressed, evolutionary novel) genes described by the authors in previous papers.

scholarly journals PVT1: A Rising Star among Oncogenic Long Noncoding RNAs

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Teresa Colombo ◽  
Lorenzo Farina ◽  
Giuseppe Macino ◽  
Paola Paci
Keyword(s):  
Noncoding Rna ◽  
Molecular Mechanisms ◽  
Functional Characterization ◽  
Noncoding Rnas ◽  
Long Noncoding Rnas ◽  
Competing Endogenous Rna ◽  
Protein Coding ◽  
Myc Oncogene ◽  
Non Coding Rnas

It is becoming increasingly clear that short and long noncoding RNAs critically participate in the regulation of cell growth, differentiation, and (mis)function. However, while the functional characterization of short non-coding RNAs has been reaching maturity, there is still a paucity of well characterized long noncoding RNAs, even though large studies in recent years are rapidly increasing the number of annotated ones. The long noncoding RNA PVT1 is encoded by a gene that has been long known since it resides in the well-known cancer risk region 8q24. However, a couple of accidental concurrent conditions have slowed down the study of this gene, that is, a preconception on the primacy of the protein-coding over noncoding RNAs and the prevalent interest in its neighbor MYC oncogene. Recent studies have brought PVT1 under the spotlight suggesting interesting models of functioning, such as competing endogenous RNA activity and regulation of protein stability of important oncogenes, primarily of the MYC oncogene. Despite some advancements in modelling the PVT1 role in cancer, there are many questions that remain unanswered concerning the precise molecular mechanisms underlying its functioning.

A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity

2018 ◽  
Vol 54 (97) ◽  
pp. 13678-13681 ◽  
Author(s):  
Dandan Zhang ◽  
Fei Ma ◽  
Junhong Leng ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Ultrasensitive Detection ◽  
Argonaute 2 ◽  
Dual Signal Amplification

We develop a new dual-signal amplification-assisted DNAzyme biosensor for sensitive detection of Argonaute 2 (Ago2) activity.

Dysregulated Expression of Long Noncoding RNAs in Endometriosis

2019 ◽  
Vol 29 (2) ◽  
pp. 113-121 ◽  
Author(s):  
Xue-ying Zhang ◽  
Lian-wen Zheng ◽  
Chun-jin Li ◽  
Ying Xu ◽  
Xu Zhou ◽  
...  
Keyword(s):  
Noncoding Rnas ◽  
Long Noncoding Rnas
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