Transcription immunoassay: light-up RNA aptamer-based immunoassay using in vitro transcription

2019 ◽  
Vol 55 (25) ◽  
pp. 3618-3621 ◽  
Author(s):  
Jieun Sim ◽  
Ju-Young Byun ◽  
Yong-Beom Shin
Keyword(s):  
Signal Amplification ◽  
In Vitro Transcription ◽  
Layer By Layer ◽  
Rna Aptamer ◽  
Biomarker Detection ◽  
First Report ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Here, we first report an ultra-enhanced immunoassay for sensitive and reliable biomarker detection using layer-by-layer assembly and transcription-assisted light-up aptamer generation to induce signal amplification.

Layer by layer assembly of biotinylated protein networks for signal amplification

2013 ◽  
Vol 49 (24) ◽  
pp. 2397 ◽  
Author(s):  
Yu W. Chu ◽  
Bo Y. Wang ◽  
Huei-Shian Lin ◽  
Tai-Yen Lin ◽  
Yuan-Jen Hung ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Protein Networks ◽  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Physical–chemical stability and in vitro digestibility of hybrid nanoparticles based on the layer-by-layer assembly of lactoferrin and BSA on liposomes

2017 ◽  
Vol 8 (4) ◽  
pp. 1688-1697 ◽  
Author(s):  
Weilin Liu ◽  
Youyu Kong ◽  
Piaohan Tu ◽  
Junmeng Lu ◽  
Chengmei Liu ◽  
...  
Keyword(s):  
Chemical Stability ◽  
Hybrid Nanoparticles ◽  
In Vitro Digestibility ◽  
Layer By Layer ◽  
Physical Chemical ◽  
Layer By Layer Assembly ◽  
Layer Deposition ◽  
Layer Assembly

Novel hybrid nanoparticles fabricated by the layer-by-layer deposition of lactoferrin and BSA on nanoliposomes showed a higher physical–chemical stability and digestibility than bare liposomes.

Antibacterial and Osteoinductive Implant Surface Using Layer-by-Layer Assembly

2021 ◽  
pp. 002203452110291
Author(s):  
M.M. Hasani-Sadrabadi ◽  
S. Pouraghaei ◽  
E. Zahedi ◽  
P. Sarrion ◽  
M. Ishijima ◽  
...  
Keyword(s):  
Stem Cells ◽  
Sustained Release ◽  
Surface Treatment ◽  
Osteogenic Differentiation ◽  
Orthopedic Implants ◽  
Layer By Layer ◽  
Implant Surface ◽  
Layer By Layer Assembly ◽  
Layer Assembly

Osseointegration of dental, craniofacial, and orthopedic implants is critical for their long-term success. Multifunctional surface treatment of implants was found to significantly improve cell adhesion and induce osteogenic differentiation of dental-derived stem cells in vitro. Moreover, local and sustained release of antibiotics via nanolayers from the surface of implants can present unparalleled therapeutic benefits in implant dentistry. Here, we present a layer-by-layer surface treatment of titanium implants capable of incorporating BMP-2–mimicking short peptides and gentamicin to improve their osseointegration and antibacterial features. Additionally, instead of conventional surface treatments, we employed polydopamine coating before layer-by-layer assembly to initiate the formation of the nanolayers on rough titanium surfaces. Cytocompatibility analysis demonstrated that modifying the titanium implant surface with layer-by-layer assembly did not have adverse effects on cellular viability. The implemented nanoscale coating provided sustained release of osteoinductive peptides with an antibacterial drug. The surface-functionalized implants showed successful osteogenic differentiation of periodontal ligament stem cells and antimicrobial activity in vitro and increased osseointegration in a rodent animal model 4 wk postsurgery as compared with untreated implants. Altogether, our in vitro and in vivo studies suggest that this approach can be extended to other dental and orthopedic implants since this surface functionalization showed improved osseointegration and an enhanced success rate.

Complex formation of modified chitosan and carboxymethyl cellulose and its effect on paper properties

TAPPI Journal ◽  
2009 ◽  
Vol 8 (6) ◽  
pp. 29-35 ◽  
Author(s):  
PEDRAM FATEHI ◽  
LIYING QIAN ◽  
RATTANA KITITERAKUN ◽  
THIRASAK RIRKSOMBOON ◽  
HUINING XIAO
Keyword(s):  
Carboxymethyl Cellulose ◽  
Polymer System ◽  
Layer By Layer ◽  
Paper Strength ◽  
Anionic Polymer ◽  
Paper Properties ◽  
Modified Chitosan ◽  
Layer By Layer Assembly ◽  
Polyelectrolyte Titration ◽  
Layer Assembly

The application of an oppositely charged dual polymer system is a promising approach to enhance paper strength. In this work, modified chitosan (MCN), a cationic polymer, and carboxymethyl cellulose (CMC), an anionic polymer, were used sequentially to improve paper strength. The adsorption of MCN on cellulose fibers was analyzed via polyelectrolyte titration. The formation of MCN/CMC complex in water and the deposition of this complex on silicon wafers were investigated by means of atomic force microscope and quasi-elastic light scattering techniques. The results showed that paper strength was enhanced slightly with a layer-by-layer assembly of the polymers. However, if the washing stage, which was required for layer-by-layer assembly, was eliminated, the MCN/CMC complex was deposited on fibers more efficiently, and the paper strength was improved more significantly. The significant improvement was attributed to the extra development of fiber bonding, confirmed further by scanning electron microscope observation of the bonding area of fibers treated with or without washing. However, the brightness of papers was somewhat decreased by the deposition of the complex on fibers. Higher paper strength also was achieved using rapid drying rather than air drying.

Stabilization of starch-based microgel-lysozyme complexes using a layer-by-layer assembly technique

2017 ◽  
Vol 214 ◽  
pp. 213-217 ◽  
Author(s):  
Bao Zhang ◽  
Yi Pan ◽  
Hanqing Chen ◽  
Tengfei Liu ◽  
Han Tao ◽  
...  
Keyword(s):  
Layer By Layer ◽  
Layer By Layer Assembly ◽  
Assembly Technique ◽  
Layer Assembly
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