Mass spectrometry imaging (MSI) of fresh bones using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

Mass spectrometry imaging is a powerful tool to analyze a large number of metabolites with their spatial coordinates collected throughout the sample. However, the significant differences in ionization efficiency pose a big challenge to metabolomic mass spectrometry imaging. To solve the challenge and obtain a complete data profile, researchers typically perform experiments in both positive and negative ionization modes, which is time-consuming. In this work, we evaluated the use of the dicationic reagent, 1,5-pentanediyl-bis(1-butylpyrrolidinium) difluoride (abbreviated to [C5(bpyr)2]F2) to detect a broad range of metabolites in the positive ionization mode by infrared matrix-assisted laser desorption electrospray ionization mass spectrometry imaging (IR-MALDESI MSI). [C5(bpyr)2]F2 at 10 µM was doped in 50% MeOH/H2O (v/v) electrospray solvent to form +1 charged adducted ions with anionic species (−1 charged) through post-electrospray ionization. This method was demonstrated with sectioned rat liver and hen ovary. A total of 73 deprotonated metabolites from rat liver tissue sections were successfully adducted with [C5(bpyr)2]2+ and putatively identified in the adducted positive ionization polarity, along with 164 positively charged metabolite ions commonly seen in positive ionization mode, which resulted in 44% increased molecular coverage. In addition, we were able to generate images of hen ovary sections showing their morphological features. Following-up tandem mass spectrometry (MS/MS) indicated that this dicationic reagent [C5(bpyr)2]2+ could form ionic bonds with the headgroup of glycerophospholipid ions. The addition of the dicationic reagent [C5(bpyr)2]2+ in the electrospray solvent provides a rapid and effective way to enhance the detection of metabolites in positive ionization mode.

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Quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissue sections by infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

The Analyst ◽

10.1039/c7an01828b ◽

2018 ◽

Vol 143 (3) ◽

pp. 654-661 ◽

Cited By ~ 18

Author(s):

Milad Nazari ◽

Mark T. Bokhart ◽

Philip L. Loziuk ◽

David C. Muddiman

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Mass Spectrometry Imaging ◽

Ovarian Tissue ◽

Desorption Electrospray Ionization ◽

Laser Desorption ◽

Quantitative Mass Spectrometry ◽

Tissue Sections ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

IR-MALDESI quantitative mass spectrometry imaging of glutathione in healthy and cancerous hen ovarian tissues.

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Infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI) mass spectrometry imaging analysis of endogenous metabolites in cherry tomatoes

The Analyst ◽

10.1039/d0an00818d ◽

2020 ◽

Vol 145 (16) ◽

pp. 5516-5523

Author(s):

M. Caleb Bagley ◽

Crystal L. Pace ◽

Måns Ekelöf ◽

David C. Muddiman

Keyword(s):

Mass Spectrometry ◽

Electrospray Ionization ◽

Mass Spectrometry Imaging ◽

Desorption Electrospray Ionization ◽

Laser Desorption ◽

Spatially Resolved ◽

Endogenous Metabolites ◽

Cherry Tomatoes ◽

Matrix Assisted Laser ◽

Matrix Assisted Laser Desorption

We report the spatially resolved metabolic profiling of cherry tomatoes using infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI); an ambient mass spectrometry imaging (MSI) technique that requires no sample derivatization.

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