An electrochemical immunosensing system on patterned electrodes for immunoglobulin E detection

2019 ◽  
Vol 11 (35) ◽  
pp. 4410-4415 ◽  
Author(s):  
Seung Jun Oh ◽  
Jun Ki Ahn ◽  
Hun Park ◽  
Yesol Song ◽  
Seong Jung Kwon ◽  
...  
Keyword(s):  
Biological Samples ◽  
Immunoglobulin E ◽  
Electrochemical Immunosensor ◽  
Electrochemical Biosensing ◽  
Patterned Electrodes ◽  
Optical Assay ◽  
Electrochemical Immunosensing

We present an electrochemical biosensing platform which enables immunoglobulin E (IgE) detection from eight independent biological samples. The performance of the electrochemical immunosensor was comparable to the conventional optical assay.

A label-free small molecular hydrogel-based electrochemical immunosensor for ultrasensitive detection of deoxynivalenol

2019 ◽  
Vol 11 (47) ◽  
pp. 5948-5952 ◽  
Author(s):  
Yu Peng ◽  
Lifang Geng ◽  
Xinyi Liu ◽  
Min Liu ◽  
Haoru Wu ◽  
...  
Keyword(s):  
Electrochemical Immunosensor ◽  
Ultrasensitive Detection ◽  
Label Free ◽  
Electrochemical Immunosensing

A guanosine-based small-molecular hydrogel is developed as a label-free electrochemical immunosensing platform for ultrasensitive detection of deoxynivalenol.

Tropomyosin Analysis in Foods Using an Electrochemical Immunosensing Approach

2021 ◽  
Vol 5 (1) ◽  
pp. 62
Author(s):  
Ricarda Torre ◽  
Maria Freitas ◽  
Estefanía Costa-Rama ◽  
Henri P. A. Nouws ◽  
Cristina Delerue-Matos
Keyword(s):  
Limit Of Detection ◽  
Polyclonal Antibodies ◽  
Analytical Signal ◽  
Food Samples ◽  
Electrochemical Immunosensor ◽  
Screen Printed Carbon Electrode ◽  
Sandwich Type ◽  
Assay Time ◽  
Electrochemical Immunosensing

A screen-printed carbon electrode was used as the transducer for the development of an electrochemical immunosensor for the determination of tropomyosin (a major shrimp allergen) in food samples. Monoclonal and polyclonal antibodies were used in a sandwich-type immunoassay. The analytical signal was electrochemically obtained using an alkaline phosphatase-labelled secondary antibody and a 3-indoxyl phosphate/silver nitrate substrate. The total assay time was 2 h 50 min and allowed the quantification of tropomyosin between 2.5 and 20 ng mL−1, with a limit of detection of 1.7 ng mL−1 The immunosensor was successfully applied to the analysis of commercial food products.

scholarly journals A new Bi2MoO6 nano-tremella-based electrochemical immunosensor for the sensitive detection of a carcinoembryonic antigen

RSC Advances ◽  
2020 ◽  
Vol 10 (27) ◽  
pp. 15870-15880 ◽  
Author(s):  
Zhongmin Wang ◽  
Xinli Tian ◽  
Dong Sun ◽  
Penghui Cao ◽  
Mengkui Ding ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Carcinoembryonic Antigen ◽  
Electrochemical Immunosensor ◽  
Sensitive Detection ◽  
Electrochemical Immunosensing

Novel Bi2MoO6 nanohybrids with a tremella-like structure modified with gold nanoparticles were used to fabricate an electrochemical immunosensing platform of CEA.

scholarly journals Hypersensitive Electrochemical Sequential Monitoring of Total IgE and their Effects with Ovalbumin Induced Mice

Proceedings ◽  
2019 ◽  
Vol 2 (13) ◽  
pp. 832
Author(s):  
JuKyung Lee ◽  
DongIm Kim ◽  
MiKyung Song ◽  
Kyuhong Lee
Keyword(s):  
Immunoglobulin E ◽  
Point Of Care ◽  
Electrochemical Immunosensor ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cytotoxic Effects ◽  
Total Ige ◽  
Alternative Approach ◽  
Incubation Periods ◽  
Reduced Costs

Monitoring of specific biomarker is critical for determining progression of a disease or efficacy of a treatment. Currently, the standard for assessing amount of specific biomarkers is the enzyme linked immunosorbent assay (ELISA), which measures quantities on the nanogram scale. However, ELISA has high material costs, long incubation periods, requires large volume of samples and involves special instruments, which necessitates clinical samples to be sent to a lab. In order to provide rapid, accurate, easy, point-of-care measurement of biomarkers, electrochemical immunosensor can be used to provide specific and sensitive biomarker detection. Immunosensor allow near real-time results, reduced costs, and simple assays with no labelling. In this work, we developed an electrochemical biosensor to measure total immunoglobulin E (IgE), a marker of asthma disease that varies with age, gender, and disease in concentrations from 0.3–1000 ng/mL with consuming 20 µL volume of real blood sample. Also, sequential monitoring of total IgE with OVA induced mice is another application of this work and this sensor is an alternative approach for recording data and a more effective assay for understanding the cytotoxic effects of toxic materials.

scholarly journals Au/NiFe2O4 nanoparticle-decorated graphene oxide nanosheets for electrochemical immunosensing of amyloid beta peptide

2020 ◽  
Vol 2 (1) ◽  
pp. 239-248 ◽  
Author(s):  
Rashmita Devi ◽  
Satyabrat Gogoi ◽  
Hemant Sankar Dutta ◽  
Manobjyoti Bordoloi ◽  
Sunil K. Sanghi ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Amyloid Beta ◽  
Glassy Carbon ◽  
Electrochemical Immunosensor ◽  
Amyloid Beta Peptide ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Graphene Oxide Nanosheets ◽  
Beta Peptide ◽  
Electrochemical Immunosensing

An electrochemical immunosensor using Au/NiFe2O4@GO-Ch nanocomposite modified glassy carbon electrode has been fabricated to detect amyloid beta peptide.

scholarly journals A Bifunctional Nanosilver-Reduced Graphene Oxide Nanocomposite for Label-Free Electrochemical Immunosensing

2021 ◽  
Vol 9 ◽  
Author(s):  
Supakeit Chanarsa ◽  
Jaroon Jakmunee ◽  
Kontad Ounnunkad
Keyword(s):  
Graphene Oxide ◽  
Reduced Graphene Oxide ◽  
Limit Of Detection ◽  
Electrochemical Immunosensor ◽  
Label Free ◽  
One Pot ◽  
Reduced Graphene ◽  
Sensitivity And Selectivity ◽  
Analytical Response ◽  
Electrochemical Immunosensing

A bi-functional material based on silver nanoparticles (AgNPs)-reduced graphene oxide (rGO) composite for both electrode modification and signal generation is successfully synthesized for use in the construction of a label-free electrochemical immunosensor. An AgNPs/rGO nanocomposite is prepared by a one-pot wet chemical process. The AgNPs/rGO composite dispersion is simply cast on a screen-printed carbon electrode (SPCE) to fabricate the electrochemical immunosensor. It possesses a sufficient conductivity/electroreactivity and improves the electrode reactivity of SPCE. Moreover, the material can generate an analytical response due to the formation of immunocomplexes for detection of human immunoglobulin G (IgG), a model biomarker. Based on electrochemical stripping of AgNPs, the material reveals signal amplification without external redox molecules/probes. Under optimized conditions, the square wave voltammetric peak current is responded to the logarithm of IgG concentration in two wide linear ranges from 1 to 50 pg.ml−1 and 0.05 to 50 ng.ml−1, and the limit of detection (LOD) is estimated to be 0.86 pg.ml−1. The proposed immunosensor displays satisfactory sensitivity and selectivity. Importantly, detection of IgG in human serum using the immunosensor shows satisfactory accuracy, suggesting that the immunosensor possesses a huge potential for further development in clinical diagnosis.

An ultrasensitive electrochemical immunosensor based on C60-modified polyamidoamine dendrimers and Au NPs for co-catalytic silver deposition

2018 ◽  
Vol 42 (6) ◽  
pp. 4653-4660 ◽  
Author(s):  
Peipei Chen ◽  
Ting Wang ◽  
Xiangli Zheng ◽  
Dong Tian ◽  
Fangquan Xia ◽  
...  
Keyword(s):  
Electrochemical Immunosensor ◽  
Silver Deposition ◽  
Au Nps ◽  
Polyamidoamine Dendrimers ◽  
Electrochemical Immunosensing

C60-Modified polyamidoamine dendrimers and Au NPs for the co-catalytic deposition of silver, used for ultrasensitive electrochemical immunosensing.

An Efficient Statistic for Determining the Diameter of Thin Sectioned Uniform Spheres from Measurements of Biological Samples

1974 ◽  
Vol 32 ◽  
pp. 114-115
Author(s):  
W. R. Schucany ◽  
G. H. Kelsoe ◽  
V. F. Allison
Keyword(s):  
Biological Samples ◽  
Traditional Method ◽  
Cell Types ◽  
Accurate Estimation ◽  
Morphological Identification ◽  
Sectioned Material ◽  
Maximal Diameter ◽  
Similar Cell

Accurate estimation of the size of spheroid organelles from thin sectioned material is often necessary, as uniquely homogenous populations of organelles such as vessicles, granules, or nuclei often are critically important in the morphological identification of similar cell types. However, the difficulty in obtaining accurate diameter measurements of thin sectioned organelles is well known. This difficulty is due to the extreme tenuity of the sectioned material as compared to the size of the intact organelle. In populations where low variance is suspected the traditional method of diameter estimation has been to measure literally hundreds of profiles and to describe the “largest” as representative of the “approximate maximal diameter”.

The Application of Ultra-Thin Sectioning Techniques to Non-Biological Samples

1968 ◽  
Vol 26 ◽  
pp. 332-333
Author(s):  
C. F. Oster
Keyword(s):  
Biological Sciences ◽  
Epoxy Resin ◽  
Cross Sections ◽  
Biological Samples ◽  
Industrial Applications ◽  
Photographic Film ◽  
Silver Particles ◽  
Thin Sectioning ◽  
Embedding Medium

Although ultra-thin sectioning techniques are widely used in the biological sciences, their applications are somewhat less popular but very useful in industrial applications. This presentation will review several specific applications where ultra-thin sectioning techniques have proven invaluable.The preparation of samples for sectioning usually involves embedding in an epoxy resin. Araldite 6005 Resin and Hardener are mixed so that the hardness of the embedding medium matches that of the sample to reduce any distortion of the sample during the sectioning process. No dehydration series are needed to prepare our usual samples for embedding, but some types require hardening and staining steps. The embedded samples are sectioned with either a prototype of a Porter-Blum Microtome or an LKB Ultrotome III. Both instruments are equipped with diamond knives.In the study of photographic film, the distribution of the developed silver particles through the layer is important to the image tone and/or scattering power. Also, the morphology of the developed silver is an important factor, and cross sections will show this structure.

Artefacts in the x-ray microanalysis of frozen-hydrated biological samples

1987 ◽  
Vol 45 ◽  
pp. 658-659
Author(s):  
Patrick Echlin
Keyword(s):  
Electron Scattering ◽  
Biological Samples ◽  
Fracture Face ◽  
Detailed Structure ◽  
X Ray ◽  
Morphological Appearance ◽  
The Poor ◽  
Freeze Dried

A number of papers have appeared recently which purport to have carried out x-ray microanalysis on fully frozen hydrated samples. It is important to establish reliable criteria to be certain that a sample is in a fully hydrated state. The morphological appearance of the sample is an obvious parameter because fully hydrated samples lack the detailed structure seen in their freeze dried counterparts. The electron scattering by ice within a frozen-hydrated section and from the surface of a frozen-hydrated fracture face obscures cellular detail. (Fig. 1G and 1H.) However, the morphological appearance alone can be quite deceptive for as Figures 1E and 1F show, parts of frozen-dried samples may also have the poor morphology normally associated with fully hydrated samples. It is only when one examines the x-ray spectra that an assurance can be given that the sample is fully hydrated.

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