A novel molecular quantitative method for rapid and sensitive detection ofEscherichia colifrom roof-harvested rainwater

2019 ◽  
Vol 11 (25) ◽  
pp. 3155-3167 ◽  
Author(s):  
Rehan Ahmed Deshmukh ◽  
Sunil Bhand ◽  
Utpal Roy
Keyword(s):  
Sensitivity And Specificity ◽  
Quantitative Method ◽  
Sensitive Detection ◽  
Agar Method ◽  
The U.S

Comparison of the sensitivity and specificity of CoDEX-qPCR with that of the U.S. EPA MI agar method 1604 anduidA,yaiOandtufgene-based qPCR.

A Triangulation Approach to Understand Multi-Cultural College Students’ Technology Literacy in a Composition Classroom

2013 ◽  
pp. 175-194
Author(s):  
Yowei Kang
Keyword(s):  
Undergraduate Students ◽  
Quantitative Method ◽  
Composition Pedagogy ◽  
Composition Classroom ◽  
Technology Literacy ◽  
Composition Classrooms ◽  
Mexico Border ◽  
Pedagogy Literature ◽  
Rhetoric Of Technology ◽  
The U.S

Despite intense debates over the use of computer and networked technologies in composition classrooms, research has been limited by one dimensional support or criticism of integrating technologies into classrooms. The inability to consider students as a central role in the literacy acquisition process has led to many problems in the rhetoric of technology as well as in the implementation of computer and networked technologies in a composition classroom. This study employed a triangulation method to gather empirical data to better assess and critique the rhetoric of technology in composition pedagogy literature. The author collected both quantitative and qualitative data to uncover issues critical to students’ technology literacy in a technologized composition classroom. A questionnaire survey was distributed to 62 bi-cultural undergraduate students conveniently recruited from a large southwestern university near the U.S.-Mexico border. Findings from the quantitative method discovered that English instructors’ technology literacy had significant impacts on students’ own technology literacy. Furthermore, narratives from the qualitative method identify the following themes about technology: effectiveness, practicality, instrumentality, and institutional enforcement. In conclusion, the author discusses the importance of technology literacy in composition classrooms to demonstrate its implications on global literacy theory and practices.

scholarly journals A novel restriction endonuclease GlaI for rapid and highly sensitive detection of DNA methylation coupled with isothermal exponential amplification reaction

2018 ◽  
Vol 9 (5) ◽  
pp. 1344-1351 ◽  
Author(s):  
Yueying Sun ◽  
Yuanyuan Sun ◽  
Weimin Tian ◽  
Chenghui Liu ◽  
Kejian Gao ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Restriction Endonuclease ◽  
Sensitivity And Specificity ◽  
Sensitive Detection ◽  
Site Specific ◽  
Highly Sensitive ◽  
Ultrahigh Sensitivity ◽  
Dna Methylations ◽  
Highly Sensitive Detection ◽  
Amplification Reaction

An elegant GlaI–EXPAR strategy is proposed which allows accurate detection of site-specific DNA methylations with ultrahigh sensitivity and specificity.

The Development of a Quantitative Method for the Detection of Periarticular Osteoporosis Using Density Features within ROIs from Computed Radiography Images of the Hand

2012 ◽  
pp. 55-67 ◽  
Author(s):  
Seiichi Murakami ◽  
Hyoungseop Kim ◽  
Joo Kooi Tan ◽  
Seiji Ishikawa ◽  
Takatoshi Aoki
Keyword(s):  
Rheumatoid Arthritis ◽  
Fourier Analysis ◽  
Diagnostic Criteria ◽  
Sensitivity And Specificity ◽  
Quantitative Method ◽  
Computed Radiography ◽  
Regions Of Interest ◽  
Histogram Analysis ◽  
Hands And Feet

Periarticular osteoporosis of the hands and feet is one of the major diagnostic criteria for rheumatoid arthritis (RA). However, a quantitative method to detect periarticular osteoporosis using radiographs has not been reported. In this chapter, the authors propose a quantitative method for the detection of periarticular osteoporosis using density features of regions of interest (ROIs) from computed radiography (CR) images of the hand. The proposed method measures the density features of ROIs using histogram analysis, co-occurrence matrices, Fourier analysis, and the extraction of line components. Periarticular osteoporosis is detected using a discernment function based on these measurements. The sensitivity and specificity of the proposed method was assessed using 188 joints from 17 cases, including 6 normal cases (without periarticular osteoporosis) and 11 abnormal cases (with periarticular osteoporosis). The sensitivity of the method was 88.9%, and the specificity was 98.1%. Therefore, the authors consider this method to be potentially useful to radiologists for detecting periarticular osteoporosis in the hands.

scholarly journals RAPID' Salmonella Chromogenic Medium

2009 ◽  
Vol 92 (6) ◽  
pp. 1871-1875 ◽  
Author(s):  
Wendy F Lauer ◽  
Frederic L Martinez ◽  
Thomas Hammack
Keyword(s):  
Sensitivity And Specificity ◽  
Emergency Response ◽  
Esterase Activity ◽  
Peanut Butter ◽  
Reference Method ◽  
Chromogenic Substrate ◽  
Salmonella Spp ◽  
Chromogenic Medium ◽  
Subsequent Recall ◽  
The U.S

Abstract RAPID' Salmonella is a chromogenic medium for isolation and detection of Salmonella spp. in food, based on two enzymatic activities. All presumptive Salmonella-positive colonies are magenta, including lactose-positive Salmonella. S. Typhi, and S. Paratyphi serotypes, due to detection of C8 esterase activity. In order to differentiate Salmonella from other Enterobacteriaceae, the medium includes a second chromogenic substrate. As part of an Emergency Response Validation due to a massive outbreak and subsequent recall, peanut butter was tested to compare the performance of RAPID' Salmonella to the U.S. Food and Drug Administration's Bacteriological Analytical Manual reference method for detection of Salmonella. Sensitivity and specificity for RAPID' Salmonella were 100.

One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)

2017 ◽  
Vol 53 (80) ◽  
pp. 11040-11043 ◽  
Author(s):  
Yuanyuan Sun ◽  
Hui Tian ◽  
Chenghui Liu ◽  
Yueying Sun ◽  
Zhengping Li
Keyword(s):  
Sensitivity And Specificity ◽  
High Sensitivity ◽  
Isothermal Amplification ◽  
Sensitive Detection ◽  
Step Detection ◽  
Loop Mediated Isothermal Amplification ◽  
Highly Sensitive ◽  
One Step ◽  
Highly Sensitive Detection

A target-triggered loop-mediated isothermal amplification (TT-LAMP) mechanism is developed for simple one-step but highly sensitive detection of microRNAs.

scholarly journals Quantitative Method To Determine Sporicidal Decontamination of Building Surfaces by Gaseous Fumigants, and Issues Related to Laboratory-Scale Studies

2009 ◽  
Vol 75 (11) ◽  
pp. 3688-3694 ◽  
Author(s):  
Vipin K. Rastogi ◽  
Lalena Wallace ◽  
Lisa S. Smith ◽  
Shawn P. Ryan ◽  
Blair Martin
Keyword(s):  
High Throughput ◽  
Environmental Protection Agency ◽  
Quantitative Method ◽  
Building Materials ◽  
Target Number ◽  
Significant Drop ◽  
Critical Issues ◽  
The Impact ◽  
The U.S ◽  
Cinder Block

ABSTRACT Chlorine dioxide gas and vaporous hydrogen peroxide sterilant have been used in the cleanup of building interiors contaminated with spores of Bacillus anthracis. A systematic study, in collaboration with the U.S. Environmental Protection Agency, was jointly undertaken by the U.S. Army-Edgewood Chemical Biological Center to determine the sporicidal efficacies of these two fumigants on six building structural materials: carpet, ceiling tile, unpainted cinder block, painted I-beam steel, painted wallboard, and unpainted pinewood. Critical issues related to high-throughput sample processing and spore recovery from porous and nonporous surfaces included (i) the extraction of spores from complex building materials, (ii) the effects of titer challenge levels on fumigant efficacy, and (iii) the impact of bioburden inclusion on spore recovery from surfaces and spore inactivation. Small pieces (1.3 by 1.3 cm of carpet, ceiling tile, wallboard, I-beam steel, and pinewood and 2.5 by 1.3 cm for cinder block) of the materials were inoculated with an aliquot of 50 μl containing the target number (1 × 106, 1 × 107, or 1 × 108) of avirulent spores of B. anthracis NNR1Δ1. The aliquot was dried overnight in a biosafety cabinet, and the spores were extracted by a combination of a 10-min sonication and a 2-min vortexing using 0.5% buffered peptone water as the recovery medium. No statistically significant drop in the kill efficacies of the fumigants was observed when the spore challenge level was increased from 6 log units to 8 log units, even though a general trend toward inhibition of fumigant efficacy was evident. The organic burden (0 to 5%) in the spore inoculum resulted in a statistically significant drop in spore recovery (at the 2 or 5% level). The effect on spore killing was a function of the organic bioburden amount and the material type. In summary, a high-throughput quantitative method was developed for determining the efficacies of fumigants, and the spore recoveries from five porous materials and one nonporous material ranged between 20 and 80%.

A Triangulation Approach to Understand Multi-Cultural College Students' Technology Literacy in a Composition Classroom

2017 ◽  
pp. 2275-2296
Author(s):  
Yowei Kang
Keyword(s):  
Undergraduate Students ◽  
Quantitative Method ◽  
Composition Pedagogy ◽  
Composition Classroom ◽  
Technology Literacy ◽  
Composition Classrooms ◽  
Mexico Border ◽  
Pedagogy Literature ◽  
Rhetoric Of Technology ◽  
The U.S

Despite intense debates over the use of computer and networked technologies in composition classrooms, research has been limited by one dimensional support or criticism of integrating technologies into classrooms. The inability to consider students as a central role in the literacy acquisition process has led to many problems in the rhetoric of technology as well as in the implementation of computer and networked technologies in a composition classroom. This study employed a triangulation method to gather empirical data to better assess and critique the rhetoric of technology in composition pedagogy literature. The author collected both quantitative and qualitative data to uncover issues critical to students' technology literacy in a technologized composition classroom. A questionnaire survey was distributed to 62 bi-cultural undergraduate students conveniently recruited from a large southwestern university near the U.S.-Mexico border. Findings from the quantitative method discovered that English instructors' technology literacy had significant impacts on students' own technology literacy. Furthermore, narratives from the qualitative method identify the following themes about technology: effectiveness, practicality, instrumentality, and institutional enforcement. In conclusion, the author discusses the importance of technology literacy in composition classrooms to demonstrate its implications on global literacy theory and practices.

scholarly journals An integrated device for the rapid and sensitive detection of the influenza hemagglutinin

Lab on a Chip ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 885-896 ◽  
Author(s):  
Caitlin E. Anderson ◽  
Joshua R. Buser ◽  
Alexis M. Fleming ◽  
Eva-Maria Strauch ◽  
Paula D. Ladd ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Sensitivity And Specificity ◽  
Microfluidic Device ◽  
Sensitive Detection ◽  
Enzymatic Amplification ◽  
Influenza Hemagglutinin ◽  
Paper Microfluidic ◽  
Integrated Device ◽  
Affinity Proteins

We demonstrate the swab-to-result detection of influenza virus using a novel paper microfluidic device. This device incorporates computationally designed affinity proteins with enzymatic amplification for improved sensitivity and specificity.

scholarly journals Dépister les cancers asymptomatiques ?

2018 ◽  
Vol 34 (4) ◽  
pp. 363-365 ◽  
Author(s):  
Bertrand Jordan
Keyword(s):  
General Population ◽  
Sensitivity And Specificity ◽  
Risk Ratio ◽  
Peripheral Blood ◽  
Liquid Biopsy ◽  
Sensitive Detection ◽  
Significant Advance ◽  
Biopsy Procedure ◽  
Different Types

A recent publication describes a sophisticated “liquid biopsy” procedure that analyses both tumoral DNA and proteins present in peripheral blood to provide sensitive detection of different types of cancer. These results represent a significant advance, but they still fall short of the sensitivity and specificity required, and it seems unlikely that they could progress to a level at which screening of the (asymptomatic) general population would have a really favourable benefit to risk ratio.

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