A robust method for critical micelle concentration determination using coumarin-6 as a fluorescent probe

2019 ◽  
Vol 11 (30) ◽  
pp. 3810-3818 ◽  
Author(s):  
Arnon Fluksman ◽  
Ofra Benny
Keyword(s):  
Critical Micelle Concentration ◽  
Fluorescent Probe ◽  
Robust Method ◽  
Simple Method ◽  
Concentration Determination ◽  
Self Assembled ◽  
Coumarin 6

Critical micelle concentration (CMC) is the point at which micelles are self-assembled from surfactants added to the system. We established a simple method based on dye micellization, using coumarin-6 as a fluorescent probe for CMC determination.

Self-assembled vesicle and rod-like aggregates of functionalized perylene diimide: reaction-based near-IR intracellular fluorescent probe for selective detection of palladium

2016 ◽  
Vol 4 (21) ◽  
pp. 3750-3759 ◽  
Author(s):  
Prabhpreet Singh ◽  
Lalit Singh Mittal ◽  
Vanita Vanita ◽  
Kapil Kumar ◽  
Amandeep Walia ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hela Cells ◽  
Perylene Diimide ◽  
Selective Detection ◽  
Near Ir ◽  
Absorbance Changes ◽  
Self Assembled ◽  
Ratiometric Absorbance

Aggregates ofPS-PDIafter Pd0based depropargylation show de-aggregation and near-IR, ratiometric absorbance changes in water and live HeLa cells.

scholarly journals Self-assembled amphiphilic fluorescent probe: detecting pH-fluctuations within cancer cells and tumour tissues

2020 ◽  
Vol 11 (36) ◽  
pp. 9875-9883 ◽  
Author(s):  
Soo Yeon Kim ◽  
Arup Podder ◽  
Hyunseung Lee ◽  
Youn-Joo Cho ◽  
Eun Hee Han ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Fluorescent Probe ◽  
Self Assembled ◽  
Ph Fluctuations

A self-assembled amphiphilic fluorescent probe allows pH-fluctuations within cancer cells and tumour tissues to be readily detected.

scholarly journals Novel fluorescent amphiphilic copolymer probes containing azo-tetraphenylethylene bridges for azoreductase-triggered release

2019 ◽  
Vol 3 (6) ◽  
pp. 1097-1104 ◽  
Author(s):  
Xiaojie Yuan ◽  
Zhe Wang ◽  
Lishan Li ◽  
Jiawei Yu ◽  
Yuqing Wang ◽  
...  
Keyword(s):  
Block Copolymer ◽  
Fluorescent Probe ◽  
Amphiphilic Copolymer ◽  
Amphiphilic Block Copolymer ◽  
Triggered Release ◽  
Azo Reductase ◽  
Self Assembled

A novel AIE fluorescent probe of amphiphilic block copolymer PCL-TPE-Azo-PEG was successfully synthesized based on azo reductase response. The polymer can be self-assembled and showed fluorescence improvement during reductant-triggered release.

A Ga3+self-assembled fluorescent probe for ATP imaging in vivo

2015 ◽  
Vol 65 ◽  
pp. 166-170 ◽  
Author(s):  
Liangliang Xiao ◽  
Shiguo Sun ◽  
Zhichao Pei ◽  
Yuxin Pei ◽  
Yi Pang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Self Assembled

A self-assembled triphenylamine-based fluorescent chemosensor for selective detection of Fe3+ and Cu2+ ions in aqueous solution

RSC Advances ◽  
2015 ◽  
Vol 5 (9) ◽  
pp. 6869-6878 ◽  
Author(s):  
Li Wenfeng ◽  
Ma Hengchang ◽  
Lu con ◽  
Ma Yuan ◽  
Qi Chunxuan ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Drinking Water ◽  
High Selectivity ◽  
Fluorescent Chemosensor ◽  
Selective Detection ◽  
Simple Method ◽  
Self Assembled ◽  
Nanomolar Range

Triphenylamine-based fluorescent chemosensor m-TAPA (S) shows high selectivity and sensitivity toward Fe3+/Cu2+ in natural and drinking water. Additionally, the TCL strips serve as a simple method for detection of Fe3+/Cu2+ at the nanomolar range.

Simple method for preparation of cubic Ag nanoparticles and their self-assembled films

2004 ◽  
Vol 460 (1-2) ◽  
pp. 78-82 ◽  
Author(s):  
D.-G. Li ◽  
S.-H. Chen ◽  
S.-Y. Zhao ◽  
X.-M. Hou ◽  
H.-Y. Ma ◽  
...  
Keyword(s):  
Ag Nanoparticles ◽  
Simple Method ◽  
Self Assembled

scholarly journals Influence factors on the critical micelle concentration determination using pyrene as a probe and a simple method of preparing samples

2020 ◽  
Vol 7 (3) ◽  
pp. 192092
Author(s):  
Hao Li ◽  
Danna Hu ◽  
Feiqing Liang ◽  
Xiaowei Huang ◽  
Qiuhua Zhu
Keyword(s):  
Critical Micelle Concentration ◽  
Sodium Dodecyl ◽  
Influence Factors ◽  
Fast Response ◽  
Exciting Light ◽  
Fluorometric Method ◽  
Average Deviation ◽  
Simple Method ◽  
Application Range

The critical micelle concentration (CMC) is an important parameter of widely used surfactants and needs to be measured in the application and development of surfactants. Fluorometric method is a widely used method determining CMC values owing to the advantages of highly sensitivity, fast response and wide application range. There are two common methods (I and II) of preparing samples for CMC fluorometric determination. In the process of developing CMC probes with aggregation-induced emission (AIE) characteristics, we found that methods I and II were not suitable for CMC probes with AIE charateristics and developed a new sample preparation method (III), which is not only suitable for CMC probes with AIE characteristic but also decreases operation procedures and errors owing to omitting the addition of micro amount of dyes into each sample. To ascertain if method III is also suitable for other CMC probes without AIE characteristics, the CMC values of surfactants were determined by fluorometric method using widely used pyrene without AIE charateristic as probe and methods I–III to prepare samples. The obtained experimental results proved that method III not only was suitable for preparation of samples for CMC determination of surfactants using pyrene as probe but also led to the least average deviation (methods I–III led to ±0.13, ±0.34 and ±0.05 mM deviation for the CMC determination of sodium dodecyl sulfate (SDS), respectively). The CMC determination using pyrene as probe is based on its change in the ratio ( I FIII / I FI ) of its emission peaks I and III with surfactant concentration. Unexpectedly, it was found that the I FIII / I FI value of pyrene in surfactant solutions is sensitive to the measurement conditions changing exciting light energy, such as slit widths and sample-measured number. In addition, it was found that surfactant SDS or cetrimonium bromide from different suppliers not only has significantly different CMC values but also leads to very different I FIII / I FI values of pyrene in a certain concentration of surfactant, which can be used as a simple method to distinguish the same surfactant with different CMC values.

scholarly journals Shear Stress-Dependent Targeting Efficiency Using Self-Assembled Gelatin–Oleic Nanoparticles in a Biomimetic Microfluidic System

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 555
Author(s):  
Taehee Kang ◽  
Chulhun Park ◽  
Nileshkumar Meghani ◽  
Thao T.D. Tran ◽  
Phuong H.L. Tran ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Flow ◽  
Cellular Uptake ◽  
Static Condition ◽  
Microfluidic System ◽  
Water Soluble ◽  
Cellular Behaviors ◽  
Self Assembled ◽  
Stress Dependent ◽  
Coumarin 6

Cellular properties and microenvironments, as well as the characteristics of nanoparticles (NPs), affect the cellular uptake and cytotoxic effects of drug-loaded NPs. Since there is fluid flow in the human blood system, fluid flow also affects the drug delivery efficiency of NPs. This study aimed to evaluate the cellular behaviors of drug-loaded soft NPs on A549 cancer cells under different levels of shear stress (0.5, 5, and 50 dynes/cm2) in the biomimetic microfluidic system. The soft self-assembled NPs were formed by the gelatin–oleic conjugate (GOC). The poorly water-soluble coumarin-6 or paclitaxel (PTX) were used as model markers for encapsulation within self-assembled NPs (C-GONs or PTX-GONs, respectively). The cellular uptake of C-GONs was found to be improved with shear-stress dependence. The inhibitory concentration (IC50) of PTX-GONs at 0.5, 5, and 50 dynes/cm2 was 0.106 µg/mL, 0.108 µg/mL, and 0.091 µg/mL, respectively, as compared to 0.138 µg/mL in a static condition. The cell killing efficiency of PTX-GONs was increased in the highest shear stress of 50 dynes/cm2 in the static condition, and other levels of shear stress in dynamic conditions.

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