Detection of alkaline phosphatase activity and inhibition with fluorescent hydroxyapatite nanoparticles

2019 ◽  
Vol 11 (17) ◽  
pp. 2272-2276 ◽  
Author(s):  
Wenqian Yang ◽  
Junjun Luo ◽  
Min Qi ◽  
Minghui Yang
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Fluorescence Detection ◽  
Alkaline Phosphatase Activity ◽  
Hydroxyapatite Nanoparticles ◽  
Alp Activity

Fluorescence detection of alkaline phosphatase (ALP) activity and inhibition based on fluorescent hydroxyapatite nanoparticles.

Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2: an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
Author(s):  
J R Farley ◽  
E Kyeyune-Nyombi ◽  
N M Tarbaux ◽  
S L Hall ◽  
D D Strong
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Lines ◽  
Alkaline Phosphatase Activity ◽  
Osteosarcoma Cell ◽  
Kinetic Assay ◽  
Human Osteosarcoma ◽  
Alp Activity ◽  
Human Osteosarcoma Cell ◽  
Skeletal Alkaline Phosphatase

Abstract Earlier we described a kinetic assay for quantifying skeletal alkaline phosphatase (ALP) isoenzyme activity in serum. The precision of the assay depends on including ALP standards for the skeletal, hepatic, intestinal, and placental isoenzymes. We wondered whether human osteosarcoma cells could provide an efficient alternative to human bone or Pagetic serum as a source of the skeletal ALP standard. ALP activities prepared from five human osteosarcoma cell lines were compared with a bone-derived ALP standard with respect to heat stability and sensitivity to chemical effectors. Two of the cell lines (SaOS-2 and TE-85) contained ALP activities that resembled the bone-derived standard. We selected SaOS-2 cells for additional evaluation (as a potential source of isoenzyme standard), because they contained 40-50 times more ALP activity than did the TE-85 cells. To include the SaOS-2 cell-derived ALP activity in the quantitative isoenzyme assay, we diluted the enzyme in a solution containing heat-inactivated (i.e., ALP-negative) human serum. Surprisingly, this dilution caused a 60-125% increase in maximum enzyme activity. In the quantitative assay of ALP isoenzyme in serum, the SaOS-2 derived ALP was indistinguishable from the serum skeletal ALP standard, with respect to the above criteria and assay variations. Evidently ALP from SaOS-2 cells is suited as a standard for measuring skeletal ALP activity in this assay.

A MnO2nanosheets–o-phenylenediamine oxidative system for the sensitive fluorescence determination of alkaline phosphatase activity

2018 ◽  
Vol 10 (44) ◽  
pp. 5341-5346 ◽  
Author(s):  
Xionghong Tan ◽  
Zheng Li ◽  
Yanlin Du ◽  
Aixian Zheng ◽  
Yongyi Zeng ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alp Activity ◽  
Fluorescence Determination ◽  
Oxidative System

A MnO2nanosheets–o-phenylenediamine (OPDA) oxidative system was developed for detecting ALP activity selectively, sensitively and conveniently.

Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation

2015 ◽  
Vol 51 (15) ◽  
pp. 3270-3272 ◽  
Author(s):  
Eun-Kyung Lim ◽  
Joo Oak Keem ◽  
Hui-suk Yun ◽  
Jinyoung Jung ◽  
Bong Hyun Chung
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Gold Nanoparticle ◽  
Alkaline Phosphatase Activity ◽  
Osteoblast Differentiation ◽  
Alp Activity

Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity.

Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3871-3877 ◽  
Author(s):  
Shengda Qi ◽  
Huanhuan Zheng ◽  
Hongyan Qin ◽  
Honglin Zhai
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Gold Nanoclusters ◽  
Inner Filter Effect ◽  
Alp Activity ◽  
Filter Effect ◽  
Sensitive Method

In this work, a simple and sensitive method based on the inner filter effect (IFE) of p-nitrophenol (PNP) on the fluorescence of gold nanoclusters (AuNCs) has been developed for detecting alkaline phosphatase (ALP) activity.

scholarly journals A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26888-26894 ◽  
Author(s):  
Jeongmoo Lee ◽  
Seoyun Kim ◽  
Tae Hoon Kim ◽  
Seoung Ho Lee
Keyword(s):  
Aqueous Solution ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Sensor ◽  
Fluorescence Assay ◽  
Quantitative Detection ◽  
Ratiometric Fluorescence ◽  
Alp Activity

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity.

scholarly journals Salivary alkaline phosphatase activity and chronological age as indicators for skeletal maturity

2019 ◽  
Vol 89 (4) ◽  
pp. 637-642
Author(s):  
Nora Alhazmi ◽  
Carroll Ann Trotman ◽  
Matthew Finkelman ◽  
Dillon Hawley ◽  
Driss Zoukhri ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Protein Concentration ◽  
Multinomial Logistic Regression ◽  
Skeletal Maturity ◽  
Chronological Age ◽  
Alp Activity ◽  
Whole Saliva ◽  
Significant Difference

ABSTRACT Objectives: To investigate the relationship between salivary alkaline phosphatase activity (ALP), protein concentration, and chronological age with cervical vertebral maturation stages (CVMS) as noninvasive biomarkers for skeletal maturity assessment. Materials and Methods: This cross-sectional study included 79 subjects (48 females, 31 males; 7 to 23 years old) categorized into five CVMS based on lateral cephalographs evaluated by three examiners. ALP activity and protein concentration in unstimulated whole saliva were compared among five CVMS. The association between age and CVMS was assessed and five multinomial logistic regression models were utilized to predict CVMS based on salivary ALP activity, protein concentration, and chronological age. Results: Salivary ALP reached the peak at early pubertal stage and then declined with a significant difference between CVMS I and CVMS II (P < .001) and between CVMS I and CVMS V (P = .004). A significant positive correlation between age and CVMS was found (rs = 0.763, P < .001). The models' overall correct classification rates for predicting CVMS were 32.9% using protein concentration, 35.4% using ALP activity, and 53.2% using both ALP activity and age. Conclusions: The combination of salivary ALP activity and chronological age may provide the best CVMS prediction.

scholarly journals An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu2+ bound to poly-thymine DNA

RSC Advances ◽  
2018 ◽  
Vol 8 (20) ◽  
pp. 11241-11246 ◽  
Author(s):  
Joon Young Lee ◽  
Jun Ki Ahn ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Phosphatase Activity ◽  
Modified Electrode ◽  
Alkaline Phosphatase Activity ◽  
Oxidation Reaction ◽  
Accurate Determination ◽  
Alp Activity ◽  
Mediated Oxidation

A novel impedimetric assay for the accurate determination of alkaline phosphatase (ALP) activity is developed based on the Cu2+-mediated oxidation of ascorbic acid on a poly-thymine DNA-modified electrode.

Facile colorimetric detection of alkaline phosphatase activity based on the target-induced valence state regulation of oxidase-mimicking Ce-based nanorods

2019 ◽  
Vol 7 (38) ◽  
pp. 5834-5841 ◽  
Author(s):  
Hongwei Song ◽  
Kun Ye ◽  
Yinxian Peng ◽  
Linjie Wang ◽  
Xiangheng Niu
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Valence State ◽  
Colorimetric Detection ◽  
State Regulation ◽  
Alp Activity ◽  
Activity Sensing

A novel ALP activity sensing strategy based on the target-induced valence state regulation of oxidase-mimicking Ce-based nanorods is proposed.

scholarly journals Serum Inorganic Phosphate and Alkaline Phosphatase Activity in Cold-Exposed Rat

2011 ◽  
Vol 19 (1) ◽  
pp. 13-17
Author(s):  
Md Shahidul Haque ◽  
Zerin Fahmida Shima
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Cold Exposure ◽  
Inorganic Phosphate ◽  
Alp Activity ◽  
Significant Change ◽  
Serum Inorganic Phosphate

To characterize serum constituents in blood in response to cold exposure, we measured serum inorganic phosphate (Pi) and alkaline phosphatase (ALP) levels. After 30 min exposure of cold, the serum Pi and alkaline phosphatase levels were 3.4 ± 0.06 mg/l and 72.33 ± 0.45 μmole/l respectively where as the control levels of these constituents are 3.05 ± 0.03 mg/l and 84 μmole/l respectively. Cold exposure for 30 min induces serum Pi concentration by 11.5 % and decreases ALP levels significantly by 13.9 % compared to control rats. Serum Pi for 1 h and 2 h were 3.8 mg/l and 4.17 ± 0.16 mg/l while ALP levels were 57.33 ± 0.88 μmole/l and 91 ± 1.73 μmole/l respectively in different groups of rats. Cold exposure stimulates serum Pi by 24.6 % and 36.7 % and reduces ALP activity by 31.7 % significantly and without significant change for 1 h and 2 h respectively. Another groups of rats exposed to cold for 4 h had Pi and ALP levels 4.66 ± 0.88 mg/l and 85.66 ± 0.88 μmole/l respectively. Cold exposure similarly stimulates Pi content significantly by 52.8 % without affecting ALP activity compared to the control rather reaches to basal level. Our results demonstrate that Pi and ALP levels in cold-exposed rats are regulated in different ways.   doi: 10.3329/taj.v19i1.3162 TAJ 2006; 19(1):13-17

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