Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation

2015 ◽  
Vol 51 (15) ◽  
pp. 3270-3272 ◽  
Author(s):  
Eun-Kyung Lim ◽  
Joo Oak Keem ◽  
Hui-suk Yun ◽  
Jinyoung Jung ◽  
Bong Hyun Chung
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Gold Nanoparticle ◽  
Alkaline Phosphatase Activity ◽  
Osteoblast Differentiation ◽  
Alp Activity

Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity.

Alkaline phosphatase activity from human osteosarcoma cell line SaOS-2: an isoenzyme standard for quantifying skeletal alkaline phosphatase activity in serum.

1989 ◽  
Vol 35 (2) ◽  
pp. 223-229 ◽  
Author(s):  
J R Farley ◽  
E Kyeyune-Nyombi ◽  
N M Tarbaux ◽  
S L Hall ◽  
D D Strong
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Cell Lines ◽  
Alkaline Phosphatase Activity ◽  
Osteosarcoma Cell ◽  
Kinetic Assay ◽  
Human Osteosarcoma ◽  
Alp Activity ◽  
Human Osteosarcoma Cell ◽  
Skeletal Alkaline Phosphatase

Abstract Earlier we described a kinetic assay for quantifying skeletal alkaline phosphatase (ALP) isoenzyme activity in serum. The precision of the assay depends on including ALP standards for the skeletal, hepatic, intestinal, and placental isoenzymes. We wondered whether human osteosarcoma cells could provide an efficient alternative to human bone or Pagetic serum as a source of the skeletal ALP standard. ALP activities prepared from five human osteosarcoma cell lines were compared with a bone-derived ALP standard with respect to heat stability and sensitivity to chemical effectors. Two of the cell lines (SaOS-2 and TE-85) contained ALP activities that resembled the bone-derived standard. We selected SaOS-2 cells for additional evaluation (as a potential source of isoenzyme standard), because they contained 40-50 times more ALP activity than did the TE-85 cells. To include the SaOS-2 cell-derived ALP activity in the quantitative isoenzyme assay, we diluted the enzyme in a solution containing heat-inactivated (i.e., ALP-negative) human serum. Surprisingly, this dilution caused a 60-125% increase in maximum enzyme activity. In the quantitative assay of ALP isoenzyme in serum, the SaOS-2 derived ALP was indistinguishable from the serum skeletal ALP standard, with respect to the above criteria and assay variations. Evidently ALP from SaOS-2 cells is suited as a standard for measuring skeletal ALP activity in this assay.

A MnO2nanosheets–o-phenylenediamine oxidative system for the sensitive fluorescence determination of alkaline phosphatase activity

2018 ◽  
Vol 10 (44) ◽  
pp. 5341-5346 ◽  
Author(s):  
Xionghong Tan ◽  
Zheng Li ◽  
Yanlin Du ◽  
Aixian Zheng ◽  
Yongyi Zeng ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Alp Activity ◽  
Fluorescence Determination ◽  
Oxidative System

A MnO2nanosheets–o-phenylenediamine (OPDA) oxidative system was developed for detecting ALP activity selectively, sensitively and conveniently.

Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect

The Analyst ◽  
2020 ◽  
Vol 145 (11) ◽  
pp. 3871-3877 ◽  
Author(s):  
Shengda Qi ◽  
Huanhuan Zheng ◽  
Hongyan Qin ◽  
Honglin Zhai
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Gold Nanoclusters ◽  
Inner Filter Effect ◽  
Alp Activity ◽  
Filter Effect ◽  
Sensitive Method

In this work, a simple and sensitive method based on the inner filter effect (IFE) of p-nitrophenol (PNP) on the fluorescence of gold nanoclusters (AuNCs) has been developed for detecting alkaline phosphatase (ALP) activity.

Abstract 300: Leptin Promotes the Osteoblast Differentiation and Mineralization of Primary Cultures of Vascular Smooth Muscle Cells by Regulating GSK-3β

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Melec G Zeadin ◽  
Martin K Butcher ◽  
Geoff H Werstuck
Keyword(s):  
Alkaline Phosphatase ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Phosphatase Activity ◽  
Vascular Calcification ◽  
Alkaline Phosphatase Activity ◽  
Osteoblast Differentiation ◽  
Muscle Cells ◽  
Dependent Manner ◽  
Gsk 3Β

Obesity is an independent risk factor for cardiovascular disease (CVD) although the precise molecular mechanisms that link obesity to CVD are not understood. Recent studies suggest that factors secreted by adipose tissue may play an adverse role in cardiovascular health. We have previously demonstrated that the adipocytokine, leptin, promotes vascular calcification in apolipoprotein E - deficient mice and that this increase in calcification is associated with an increase in the expression of several osteoblast-specific markers within the vessel wall. In an effort to understand the mechanism by which leptin exerts these effects, we cultured primary bovine aortic smooth muscle cells (BASMCs) in the presence of 0 to 2 μg/ml leptin for up to 12 days. Osteogenic differentiation of BASMCs was determined by an increase in the expression of osteoblast-specific markers, and the induction of both alkaline phosphatase activity and mineralization. Consistent with previous studies, we found that treatment of BASMCs with leptin induced osteoblast differentiation in a dose-dependent manner. To investigate the underlying mechanism, we examined changes in the expression levels of key factors implicated in osteoblast differentiation, including members of the Wnt signaling pathway. We found that exposure to leptin induced the Erk 1/2-dependent inactivation of GSK-3β, through Ser9 phosphorylation, and a subsequent increase in the nuclear accumulation of β-catenin. Transfection of BASMCs with an adenovirus that expressed constitutively active GSK-3β (Ad-GSK-3β S9A) resulted in a > 2-fold increase in GSK-3β activity, a decrease in the expression of the osteoblast-specific marker, osteopontin, and a significant decrease in leptin-induced alkaline phosphatase activity. Together, our results provide a possible mechanism by which elevated leptin concentrations, associated with obesity, promote osteoblast differentiation, and vascular calcification in vivo.

scholarly journals A ratiometric fluorescence sensor based on enzymatically activatable micellization of TPE derivatives for quantitative detection of alkaline phosphatase activity in serum

RSC Advances ◽  
2020 ◽  
Vol 10 (45) ◽  
pp. 26888-26894 ◽  
Author(s):  
Jeongmoo Lee ◽  
Seoyun Kim ◽  
Tae Hoon Kim ◽  
Seoung Ho Lee
Keyword(s):  
Aqueous Solution ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Sensor ◽  
Fluorescence Assay ◽  
Quantitative Detection ◽  
Ratiometric Fluorescence ◽  
Alp Activity

A novel ratiometric fluorescence assay via enzymatically activatable micellization in aqueous solution was devised for quantitative detection of alkaline phosphatase (ALP) activity.

scholarly journals Salivary alkaline phosphatase activity and chronological age as indicators for skeletal maturity

2019 ◽  
Vol 89 (4) ◽  
pp. 637-642
Author(s):  
Nora Alhazmi ◽  
Carroll Ann Trotman ◽  
Matthew Finkelman ◽  
Dillon Hawley ◽  
Driss Zoukhri ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Protein Concentration ◽  
Multinomial Logistic Regression ◽  
Skeletal Maturity ◽  
Chronological Age ◽  
Alp Activity ◽  
Whole Saliva ◽  
Significant Difference

ABSTRACT Objectives: To investigate the relationship between salivary alkaline phosphatase activity (ALP), protein concentration, and chronological age with cervical vertebral maturation stages (CVMS) as noninvasive biomarkers for skeletal maturity assessment. Materials and Methods: This cross-sectional study included 79 subjects (48 females, 31 males; 7 to 23 years old) categorized into five CVMS based on lateral cephalographs evaluated by three examiners. ALP activity and protein concentration in unstimulated whole saliva were compared among five CVMS. The association between age and CVMS was assessed and five multinomial logistic regression models were utilized to predict CVMS based on salivary ALP activity, protein concentration, and chronological age. Results: Salivary ALP reached the peak at early pubertal stage and then declined with a significant difference between CVMS I and CVMS II (P < .001) and between CVMS I and CVMS V (P = .004). A significant positive correlation between age and CVMS was found (rs = 0.763, P < .001). The models' overall correct classification rates for predicting CVMS were 32.9% using protein concentration, 35.4% using ALP activity, and 53.2% using both ALP activity and age. Conclusions: The combination of salivary ALP activity and chronological age may provide the best CVMS prediction.

scholarly journals An impedimetric determination of alkaline phosphatase activity based on the oxidation reaction mediated by Cu2+ bound to poly-thymine DNA

RSC Advances ◽  
2018 ◽  
Vol 8 (20) ◽  
pp. 11241-11246 ◽  
Author(s):  
Joon Young Lee ◽  
Jun Ki Ahn ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Alkaline Phosphatase ◽  
Ascorbic Acid ◽  
Phosphatase Activity ◽  
Modified Electrode ◽  
Alkaline Phosphatase Activity ◽  
Oxidation Reaction ◽  
Accurate Determination ◽  
Alp Activity ◽  
Mediated Oxidation

A novel impedimetric assay for the accurate determination of alkaline phosphatase (ALP) activity is developed based on the Cu2+-mediated oxidation of ascorbic acid on a poly-thymine DNA-modified electrode.

Facile colorimetric detection of alkaline phosphatase activity based on the target-induced valence state regulation of oxidase-mimicking Ce-based nanorods

2019 ◽  
Vol 7 (38) ◽  
pp. 5834-5841 ◽  
Author(s):  
Hongwei Song ◽  
Kun Ye ◽  
Yinxian Peng ◽  
Linjie Wang ◽  
Xiangheng Niu
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Valence State ◽  
Colorimetric Detection ◽  
State Regulation ◽  
Alp Activity ◽  
Activity Sensing

A novel ALP activity sensing strategy based on the target-induced valence state regulation of oxidase-mimicking Ce-based nanorods is proposed.

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