High sensitivity detection of Escherichia coli based on the measurement of β-galactosidase activity by microchip capillary electrophoresis combined with field-amplified sample injection

2019 ◽  
Vol 11 (11) ◽  
pp. 1558-1565 ◽  
Author(s):  
Yan Zhang ◽  
Yating Zhang ◽  
Luqi Zhu ◽  
Pingang He ◽  
Qingjiang Wang
Keyword(s):  
Escherichia Coli ◽  
Capillary Electrophoresis ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Galactosidase Activity ◽  
Microchip Capillary Electrophoresis ◽  
Sample Injection ◽  
E Coli ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

A sensitive strategy developed for the detection of Escherichia coli (E. coli) by microchip capillary electrophoresis (MCE) combined with laser-induced fluorescence (LIF) is described in this paper.

Simultaneous Detection of Yersinia Enterocolitica and Listeria Monocytogenes in Foodstuffs by Capillary Electrophoresis and Microchip Capillary Electrophoresis Laser-Induced Fluorescence Detector

2018 ◽  
Vol 101 (6) ◽  
pp. 1833-1838 ◽  
Author(s):  
Yongru Li ◽  
Hongwei Su ◽  
Yajia Lan
Keyword(s):  
Capillary Electrophoresis ◽  
Listeria Monocytogenes ◽  
Yersinia Enterocolitica ◽  
Simultaneous Detection ◽  
High Sensitivity ◽  
Laser Induced Fluorescence ◽  
Microchip Capillary Electrophoresis ◽  
Fluorescence Detector ◽  
Sybr Gold ◽  
Pcr Products

Abstract Background: Food safety is one of the most important public health problems in the world, and pathogenic bacterium is a major factor causing serious foodborne diseases. Objective: Two methods of duplex PCR combined with capillary electrophoresis laser-induced fluorescence detector (CE-LIF) and microchip capillary electrophoresis laser-induced fluorescence detector (MCE-LIF) have been developed for the simultaneous detection of Yersinia Enterocolitica and Listeria Monocytogenes in various foods. The specific conservative sequences of these two bacteria were amplified. Methods: After labelled with nucleic acid dye SYBR Gold and SYBR Orange, the PCR products were analyzed by CE-LIF and MCE-LIF, respectively. Under the optimal conditions, the detection of PCR products of the target bacteria was achieved in less than 15 min by CE-LIF and within 6 min by MCE-LIF. Results: The alignment analysis demonstrated that the PCR products had good agreement with the sequences published in GenBank. The CE-LIF method could detect 10 CFU/mL Y. enterocolitica and L. monocytogenes, and the MCE-LIF method could detect 100 CFU/mL Y. enterocolitica and L. monocytogenes. The intraday precisions of migration time and peak area of DNA markers and PCR products were in the range of 1.13 to 1.18% and 1.60 to 6.29%, respectively, for CE-LIF and 1.18 to 1.48% and 2.85 to 4.06%, respectively, for MCE-LIF. Conclusions: The proposed methods could be applied to target bacterial detection infood samples rapidly, sensitively, and specifically. Highlights: Two new methods based on CE and MCE have been developed for the simultaneous detection of Y. enterocolitica and L. monocytogenes in foodstuffs, and they can detect the bacteria directly without any enrichment because of their high sensitivity.

High-sensitivity detection of capillary electrophoresis with multiphoton excitation

2002 ◽  
Author(s):  
Ling Fu ◽  
Qiang Lu ◽  
Hong Chen ◽  
Tongsheng Chen ◽  
Shaoqun Zeng ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
High Sensitivity ◽  
Multiphoton Excitation ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

scholarly journals High-sensitivity detection of TNT

2006 ◽  
Vol 103 (52) ◽  
pp. 19630-19634 ◽  
Author(s):  
M. B. Pushkarsky ◽  
I. G. Dunayevskiy ◽  
M. Prasanna ◽  
A. G. Tsekoun ◽  
R. Go ◽  
...  
Keyword(s):  
High Sensitivity ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

scholarly journals Photostable phosphorescent polymer nanospheres for high sensitivity detection

2015 ◽  
Vol 3 (25) ◽  
pp. 6565-6572 ◽  
Author(s):  
A. N. Edwards ◽  
M. Yamazaki ◽  
S. H. Krishnadasan ◽  
T. W. Phillips ◽  
L. Rowlands ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Simple Method ◽  
Polymer Nanospheres ◽  
High Sensitivity Detection ◽  
Sensitivity Detection

A simple method is presented for the preparation of photostable phosphorescent polymer nanospheres.

Simple and high-sensitivity detection of dioxin using dioxin-binding pentapeptide

2007 ◽  
Vol 22 (9-10) ◽  
pp. 2093-2099 ◽  
Author(s):  
Yasuhiro Inuyama ◽  
Chikashi Nakamura ◽  
Tomohiro Oka ◽  
Yasuhiro Yoneda ◽  
Ikuo Obataya ◽  
...  
Keyword(s):  
High Sensitivity ◽  
High Sensitivity Detection ◽  
Sensitivity Detection
Sign in / Sign up

Export Citation Format

Share Document