A chip-based potentiometric sensor for a Zika virus diagnostic using 3D surface molecular imprinting

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4266-4280 ◽  
Author(s):  
Vincent Ricotta ◽  
Yingjie Yu ◽  
Nicholas Clayton ◽  
Ya-Chen Chuang ◽  
Yantian Wang ◽  
...  
Keyword(s):  
Molecular Imprinting ◽  
Zika Virus ◽  
High Selectivity ◽  
Detection System ◽  
Point Of Care ◽  
Lab On A Chip ◽  
High Sensitivity ◽  
Potentiometric Sensor ◽  
Surface Molecular Imprinting ◽  
3D Surface

The high sensitivity and high selectivity demonstrated here proved that this lab-on-a-chip diagnostic has the potential to become a point-of-care (POC) detection system for rapid and accurate screening of flaviviruses.

Quantitative real-time detection of carcinoembryonic antigen (CEA) from pancreatic cyst fluid using 3-D surface molecular imprinting

The Analyst ◽  
2016 ◽  
Vol 141 (14) ◽  
pp. 4424-4431 ◽  
Author(s):  
Yingjie Yu ◽  
Qi Zhang ◽  
Jonathan Buscaglia ◽  
Chung-Chueh Chang ◽  
Ying Liu ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Real Time ◽  
Molecular Imprinting ◽  
Pancreatic Cyst ◽  
Cyst Fluid ◽  
Surface Molecular Imprinting ◽  
3D Surface ◽  
Real Time Detection ◽  
Pancreatic Cyst Fluid

In this study, a real time potentiometric biosensor based on the 3D surface molecular imprinting was developed for CEA detection.

scholarly journals The Fabrication of Amino Acid Incorporated Nanoflowers with Intrinsic Peroxidase-like Activity and Its Application for Efficiently Determining Glutathione with TMB Radical Cation as Indicator

Micromachines ◽  
2021 ◽  
Vol 12 (9) ◽  
pp. 1099
Author(s):  
Ning Jiang ◽  
Chuang Zhang ◽  
Meng Li ◽  
Shuai Li ◽  
Zhili Hao ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Selectivity ◽  
Storage Stability ◽  
Mechanical Stability ◽  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Detection System ◽  
High Sensitivity ◽  
Peroxidase Mimics ◽  
Application Potential

The assessment of glutathione (GSH) levels is associated with early diagnostics and pathological analysis for various disorders. Among all kinds of techniques for detecting GSH, the colorimetric assay relying on the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) catalyzed by many nanomaterials with peroxidase-like activity attracts increasing attention owing to its outstanding merits, such as high sensitivity and high selectivity. However, the aggregation between the nanomaterials severely hinders the entrance of TMB into the “active site” of these peroxidase mimics. To address this problem, the D-amino acid incorporated nanoflowers possessing peroxidase-like activity with a diameter of 10–15 μm, TMB and H2O2 were employed to establish the detection system for determining the level of glutathione. The larger diameter size of the hybrid nanoflowers substantially averts the aggregation between them. The results confirm that the hybrid nanoflowers detection system presents a low limit of detection, wide linear range, perfect selectivity, good storage stability and desired operational stability for the detection of GSH relying on the intrinsic peroxidase-like activity and favorable mechanical stability of the hybrid nanoflowers, indicating that the hybrid nanoflowers detection system has tremendous application potential in clinical diagnosis and treatment.

scholarly journals Highly Specific and Sensitive Detection of Yersinia pestis by Portable Cas12a-UPTLFA Platform

2021 ◽  
Vol 12 ◽  
Author(s):  
Yang You ◽  
Pingping Zhang ◽  
Gengshan Wu ◽  
Yafang Tan ◽  
Yong Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Yersinia Pestis ◽  
Rapid Detection ◽  
Detection Method ◽  
Detection System ◽  
Point Of Care ◽  
High Sensitivity ◽  
Nucleic Acid Detection ◽  
Great Promise ◽  
High Background

The recent discovery of collateral cleavage activity of class-II clustered regularly interspaced short palindromic repeats–CRISPR-associated protein (CRISPR-Cas) makes CRISPR-based diagnosis a potential high-accuracy nucleic acid detection method. Colloidal gold-based lateral flow immunochromatographic assay (LFA), which has been combined with CRISPR/Cas-based nucleic detection, usually associates with drawbacks of relative high background and the subjectivity in naked-eye read-out of the results. Here, we developed a novel system composed of Cas12a-based nucleic acid detection and up-converting phosphor technology (UPT)-based LFA (UPT–LFA), termed Cas12a-UPTLFA. We further demonstrated the utility of this platform in highly sensitive and specific detection of Yersinia pestis, the causative agent of the deadly plague. Due to high infectivity and mortality, as well as the potential to be misused as bioterrorism agent, a culture-free, ultrasensitive, specific, and rapid detection method for Y. pestis has long been desired. By incorporating isothermal recombinase polymerase amplification, the Cas12a-UPTLFA we established can successfully detect genomic DNA of Y. pestis as low as 3 attomolar (aM) and exhibited high sensitivity (93.75%) and specificity (90.63%) for detection of spiked blood samples with a detection limit of 102 colony-forming unit per 100 μl of mouse blood. With a portable biosensor, Cas12a-UPTLFA assay can be operated easily by non-professional personnel. Taken together, we have developed a novel Cas12a-UPTLFA platform for rapid detection of Y. pestis with high sensitivity and specificity, which is portable, not expensive, and easy to operate as a point-of-care method. This detection system can easily be extended to detect other pathogens and holds great promise for on-site detection of emerging infectious pathogens.

Reusable Surface Molecular Imprint Biosensors Aided by Naturally Occurring Surface Roughness Indices for Point-of-Care Diagnostics

MRS Advances ◽  
2019 ◽  
Vol 4 (22) ◽  
pp. 1299-1308 ◽  
Author(s):  
Yehoshua Auerbach ◽  
Rebecca Isseroff ◽  
Nicholas Clayton ◽  
Miguel Hulyalkar ◽  
Andrew Todt ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Imprinting ◽  
Point Of Care ◽  
High Sensitivity ◽  
Cost Effective ◽  
Stem Cell Differentiation ◽  
Diverse Range ◽  
Point Of Care Diagnostics ◽  
Molecular Imprint ◽  
Detection Of Biomarkers

ABSTRACTWe have shown that molecular imprinting (MI) technology can be used to produce sensitive, robust, cost-effective biosensing systems with a real-time electrochemical readout that can be utilized for point of care diagnostics. Real time detection of biomarkers is essential when rapid, critical decisions need to be made, such as in situations where public health is threatened. Our biosensor has high sensitivity compared to standard methods like ELISA, and results are obtained within minutes, using inexpensive, accessible potentiometric readout technology. These biosensors utilize surface molecular imprinting of a self-assembling monolayer of hydroxy-terminated alkanethiol chains which form a crystalline ‘lock-and-key’ structure around a target analyte, allowing the sensors to detect and differentiate between bio-macromolecules of similar size and shape with high selectivity and sensitivity. The sensors are extremely versatile and able to detect a diverse range of molecules of varied chemical composition and structure. To fully exploit the sensors’ advantages, especially in remote, economically disadvantaged areas, it is important to quantify their durability and reusability. Biosensor chips were created to test the viability of hemoglobin detection and to evaluate the potential for sensor reusability when washed after detection testing. The successful readsorption of hemoglobin even after washing, accompanied by cyclic voltammetry data indicating the preservation of the SAM, indicate that these biosensors are reusable, significantly augmenting the device’s value. Potential applications include the analysis of complex chemical and biological processes such as stem cell differentiation and on-the-spot detection of diseases such as Zika.

Molecularly imprinted polymers’ application in pesticide residue detection

The Analyst ◽  
2018 ◽  
Vol 143 (17) ◽  
pp. 3971-3989 ◽  
Author(s):  
Saqib Farooq ◽  
Jiyun Nie ◽  
Yang Cheng ◽  
Zhen Yan ◽  
Jing Li ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Pesticide Residue ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
High Selectivity ◽  
High Sensitivity ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Residue Detection ◽  
Molecular Imprinting Technology

Molecularly imprinted polymers (MIPs) are produced using molecular imprinting technology (MIT) and have specific analyte-binding abilities and unique properties, including chemical and thermal stability, reusability, high selectivity, and high sensitivity.

Giant Magnetoresistive Based Handheld System for Rapid Detection of Human NT-proBNP

2019 ◽  
Author(s):  
Wei Wang ◽  
Todd Klein ◽  
James Collins
Keyword(s):  
Limit Of Detection ◽  
Detection System ◽  
Point Of Care ◽  
High Sensitivity ◽  
Time Signal ◽  
Point Of Care Testing ◽  
Detection Range ◽  
Further Development ◽  
Signal Readout

In this work, we developed giant magnetoresistive (GMR) based handheld biosensing systems that serve as platform for detecting human NT-proBNP. This assay takes advantages of high sensitivity and real-time signal readout of GMR biosensor. The limit of detection was estimated to be less than 0.01ng/mL, and detection range covered from 0.01 ng/mL to 5 ng/mL was obtained. The assay can be completed within 20 min, which is very important for further development of point-of-care testing. The proposed GMR handheld system is also successfully used for the detection of real NT-proBNP human samples. It can be foreseen that this handheld detection system could become a robust contender in the applications of in vitro biomarker diagnostics.

Surface molecular imprinting on CdTe quantum dots for fluorescence sensing of 4-nitrophenol

2016 ◽  
Vol 8 (10) ◽  
pp. 2226-2232 ◽  
Author(s):  
Linye Jiang ◽  
Haijian Liu ◽  
Ming Li ◽  
Yue Xing ◽  
Xueqin Ren
Keyword(s):  
Quantum Dots ◽  
Detection Limit ◽  
Molecular Imprinting ◽  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Surface Molecular Imprinting ◽  
Fluorescence Sensing ◽  
Lower Detection Limit ◽  
Lower Detection ◽  
Sensitivity And Selectivity

The developed fluorescent sensor provides high sensitivity and selectivity and a lower detection limit than previously developed sensors.

Novel High Sensitivity Tuberculosis Point-of-Care Test for People Living with HIV

2018 ◽  
Author(s):  
Tobias Broger ◽  
Bianca Sossen ◽  
Elloise du Toit ◽  
Andrew D. Kerkhoff ◽  
Charlotte Schutz ◽  
...  
Keyword(s):  
Point Of Care ◽  
High Sensitivity ◽  
People Living With Hiv ◽  
Point Of Care Test ◽  
Living With Hiv

Trends in Diagnosis for Active Tuberculosis Using Nanomaterials

2019 ◽  
Vol 26 (11) ◽  
pp. 1946-1959 ◽  
Author(s):  
Le Minh Tu Phan ◽  
Lemma Teshome Tufa ◽  
Hwa-Jung Kim ◽  
Jaebeom Lee ◽  
Tae Jung Park
Keyword(s):  
Sensitivity And Specificity ◽  
High Efficiency ◽  
Point Of Care ◽  
High Sensitivity ◽  
Signs And Symptoms ◽  
Diagnostic Methods ◽  
Advantages And Disadvantages ◽  
Treatment And Prevention ◽  
Clinical Tools ◽  
Diagnostic Applications

Background:Tuberculosis (TB), one of the leading causes of death worldwide, is difficult to diagnose based only on signs and symptoms. Methods for TB detection are continuously being researched to design novel effective clinical tools for the diagnosis of TB.Objective:This article reviews the methods to diagnose TB at the latent and active stages and to recognize prospective TB diagnostic methods based on nanomaterials.Methods:The current methods for TB diagnosis were reviewed by evaluating their advantages and disadvantages. Furthermore, the trends in TB detection using nanomaterials were discussed regarding their performance capacity for clinical diagnostic applications.Results:Current methods such as microscopy, culture, and tuberculin skin test are still being employed to diagnose TB, however, a highly sensitive point of care tool without false results is still needed. The utilization of nanomaterials to detect the specific TB biomarkers with high sensitivity and specificity can provide a possible strategy to rapidly diagnose TB. Although it is challenging for nanodiagnostic platforms to be assessed in clinical trials, active TB diagnosis using nanomaterials is highly expected to achieve clinical significance for regular application. In addition, aspects and future directions in developing the high-efficiency tools to diagnose active TB using advanced nanomaterials are expounded.Conclusion:This review suggests that nanomaterials have high potential as rapid, costeffective tools to enhance the diagnostic sensitivity and specificity for the accurate diagnosis, treatment, and prevention of TB. Hence, portable nanobiosensors can be alternative effective tests to be exploited globally after clinical trial execution.

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