Rapid isolation of cfDNA from large-volume whole blood on a centrifugal microfluidic chip based on immiscible phase filtration

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4162-4174 ◽  
Author(s):  
Fei Hu ◽  
Juan Li ◽  
Niancai Peng ◽  
Zheng Li ◽  
Zengming Zhang ◽  
...  
Keyword(s):  
Large Volume ◽  
Microfluidic Chip ◽  
Whole Blood ◽  
Rapid Isolation ◽  
Immiscible Phase

The C-IFAST device enabled the rapid isolation of cfDNA, from 4 ml whole blood to 50 μl elution, within 15 min.

Cardiac Troponin I Microfluidic Chip Driven by Adaptive Pressure

2020 ◽  
Vol 12 (10) ◽  
pp. 1239-1247
Author(s):  
Xingshang Xu ◽  
Yuan Liu ◽  
Zhu Chen ◽  
Hui Chen ◽  
Yan Deng ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Whole Blood ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Accurate Determination ◽  
Cardiac Troponin I ◽  
Chip Surface ◽  
Layer Composite ◽  
Novel Strategy ◽  
The Stability

To develop and design an adaptive microfluidic chip for accurate determination of cardiac troponin I (cTnI) in whole blood sample and explore the operating parameters of the chip in detecting cTnI, in order to provide a novel strategy for the detection of cTnI, cTnI microfluidic chip was prepared by injection moulding, and the improved polystyrene polymer was used as the chip substrate to construct a three-layer composite structure, namely the upper, middle, and lower layers. The antihuman troponin I antibody I/II was grafted onto the chip surface to construct the detection reaction zone using UV-induced production of surface-active free radicals. The stability of the chip preparation process, the running performance of the chip, and the analytical performance of the whole blood samples were investigated. It was shown that I adaptive pressure-driven microfluidic chip has the advantages of easy bonding, integration, and a simple and stable production process. In the actual detection and analysis, the chip has high selectivity for cTnI in whole blood, lower detection limit (0.054 ng/mL), and small difference between batches (RSD% 2.50%). Therefore, the chip is assumed to provide novel strategy for the assessment of myocardial infarction by detecting cTnI.

Pneumatic-pumping metering in CD-like microfluidic chip for whole blood analysis

2014 ◽  
Vol 22 (10) ◽  
pp. 2733-2739 ◽  
Author(s):  
范建华 FAN Jian-hua ◽  
邓永波 DENG Yong-bo ◽  
宣明 XUAN Ming ◽  
周松 ZHOU Song ◽  
武俊峰 WU Jun-feng ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Whole Blood ◽  
Blood Analysis ◽  
Whole Blood Analysis

Isolation of circulating fetal trophoblasts by a four-stage inertial microfluidic device for single-cell analysis and noninvasive prenatal testing

Lab on a Chip ◽  
2020 ◽  
Vol 20 (23) ◽  
pp. 4342-4348
Author(s):  
Yifang Huang ◽  
Sheng Yu ◽  
Shuzhe Chao ◽  
Limei Wu ◽  
Maliang Tao ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Whole Blood ◽  
Single Cell Analysis ◽  
Low Cost ◽  
Prenatal Testing ◽  
Cell Analysis ◽  
Noninvasive Prenatal Testing ◽  
Blood Samples ◽  
Trophoblastic Cells ◽  
Whole Blood Samples

A novel four-stage inertial microfluidic chip is developed for isolating rare circulating trophoblastic cells from whole blood samples of pregnancies. The antibody-free, low-cost assay may serve as a useful platform for noninvasive prenatal testing.

Device for whole genome sequencing single circulating tumor cells from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3168-3178 ◽  
Author(s):  
Ren Li ◽  
Fei Jia ◽  
Weikai Zhang ◽  
Fanghao Shi ◽  
Zhiguo Fang ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Genome Sequencing ◽  
Microfluidic Chip ◽  
Whole Blood ◽  
Whole Genome

To sequence single circulating tumor cells (CTCs) from whole blood, a microfluidic chip was developed to perform blood filtering/CTC enrichment/CTC sorting and in situ MDA for whole genome sequencing.

A method for large volume blood sampling and transfusion in rats

1986 ◽  
Vol 250 (3) ◽  
pp. E331-E337
Author(s):  
H. R. Berthoud ◽  
W. B. Laughton ◽  
T. L. Powley
Keyword(s):  
Large Volume ◽  
Whole Blood ◽  
Superior Vena ◽  
Vena Cava ◽  
Blood Sampling ◽  
Indwelling Catheters ◽  
Multiple Channel ◽  
Glucose Levels ◽  
Include Blood Pressure ◽  
Volume Blood

A new protocol that makes it feasible to withdraw large volumes of whole blood from an individual rat within 1 h or less is described. This method involves the use of indwelling catheters for withdrawal of blood from the inferior vena cava with concurrent isovolemic replacement of whole blood into the superior vena cava. Simultaneity of the transfusion and withdrawal, strict equality of volumes, and a smooth exchange of blood are assured by the use of separate channels of the same multiple-channel peristaltic pump for withdrawal and replacement. Validation experiments using both anesthetized and unanesthetized rats indicate that several responses remain essentially undisturbed during large volume blood sampling; these parameters include blood pressure, heart rate, hematocrit, plasma hormones including insulin and glucagon, plasma glucose levels, and feeding behavior. Considerations of technical and physiological limitations of the protocol, including choice of catheters and catheter placement, pump, sampling parameters, and obtaining donor blood, are discussed.

Large volume whole blood mononuclear cell enrichment using a COBE spectra

Cytotherapy ◽  
2013 ◽  
Vol 15 (4) ◽  
pp. S50
Author(s):  
N. Almezel ◽  
S. Pinkard ◽  
T. Leemhuis
Keyword(s):  
Mononuclear Cell ◽  
Large Volume ◽  
Whole Blood ◽  
Cell Enrichment
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