An innovative silicon-chip for sensitive real time PCR improvement in pathogen detection

The Analyst ◽  
2019 ◽  
Vol 144 (7) ◽  
pp. 2353-2358 ◽  
Author(s):  
Simone Battaglia ◽  
Salvatore Petralia ◽  
Nunzio Vicario ◽  
Daniela Cirillo ◽  
Sabrina Conoci
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pathogen Detection ◽  
Sensitive Detection ◽  
Qrt Pcr ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

An innovative miniaturized silicon-chip was developed for highly sensitive detection of pathogen genomes of both viruses and bacteria through real time PCR (qRT-PCR).

scholarly journals Novel One-Step Single-Tube Nested Quantitative Real-Time PCR Assay for Highly Sensitive Detection of SARS-CoV-2

2020 ◽  
Vol 92 (13) ◽  
pp. 9399-9404 ◽  
Author(s):  
Ji Wang ◽  
Kun Cai ◽  
Ruiqing Zhang ◽  
Xiaozhou He ◽  
Xinxin Shen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Highly Sensitive ◽  
One Step ◽  
Highly Sensitive Detection

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

A supersensitive biosensor based on MoS2 nanosheet arrays for the real-time detection of H2O2 secreted from living cells

2019 ◽  
Vol 55 (65) ◽  
pp. 9653-9656 ◽  
Author(s):  
Huitong Du ◽  
Xinyue Zhang ◽  
Zhe Liu ◽  
Fengli Qu
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Sensitive Detection ◽  
Live Cells ◽  
The Real ◽  
Highly Sensitive ◽  
Nanosheet Arrays ◽  
Mos2 Nanosheet ◽  
Real Time Detection ◽  
Highly Sensitive Detection

A self-supported MoS2 nanosheet biosensor for highly sensitive detection of H2O2 secreted from live cells.

Highly sensitive conjugated polymer fluorescent sensors based on benzochalcogendiazole for nickel ions in real-time detection

2014 ◽  
Vol 2 (35) ◽  
pp. 7402-7410 ◽  
Author(s):  
Yunxiang Lei ◽  
Hui Li ◽  
Wenxia Gao ◽  
Miaochang Liu ◽  
Jiuxi Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Conjugated Polymer ◽  
Fluorescent Sensors ◽  
Sensitive Detection ◽  
Nickel Ions ◽  
Highly Sensitive ◽  
Real Time Detection ◽  
Highly Sensitive Detection

Two conjugated polymer fluorescent sensors using a benzochalcogendiazole unit and a triazole unit as cooperative receptors were synthesized for the highly sensitive detection of nickel ions.

scholarly journals Highly Sensitive GMO Detection Using Real-Time PCR with a Large Amount of DNA Template: Single-Laboratory Validation

2018 ◽  
Vol 101 (2) ◽  
pp. 507-514 ◽  
Author(s):  
Junichi Mano ◽  
Shuko Hatano ◽  
Yasuaki Nagatomi ◽  
Satoshi Futo ◽  
Reona Takabatake ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Detection Methods ◽  
35S Promoter ◽  
Processed Foods ◽  
Gmo Detection ◽  
Nos Terminator ◽  
Highly Sensitive ◽  
Dna Template

Abstract Current genetically modified organism (GMO) detection methods allow for sensitive detection. However, a further increase in sensitivity will enable more efficient testing for large grain samples and reliable testing for processed foods. In this study, we investigated real-time PCR-based GMO detection methods using a large amount of DNA template. We selected target sequences that are commonly introduced into many kinds of GM crops, i.e., 35S promoter and nopaline synthase (NOS) terminator. This makes the newly developed method applicable to a wide range of GMOs, including some unauthorized ones. The estimated LOD of the new method was 0.005% of GM maize events; to the best of our knowledge, this method is the most sensitive among the GM maize detection methods for which the LOD was evaluated in terms of GMO content. A 10-fold increase in the DNA amount as compared with the amount used under common testing conditions gave an approximately 10-fold reduction in the LOD without PCR inhibition. Our method is applicable to various analytical samples, including processed foods. The use of other primers and fluorescence probes would permit highly sensitive detection of various recombinant DNA sequences besides the 35S promoter and NOS terminator.

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