Smartly designed AIE triazoliums as unique targeting fluorescence tags for sulfonic biomacromolecule recognition via ‘electrostatic locking’

2018 ◽  
Vol 6 (46) ◽  
pp. 12529-12536 ◽  
Author(s):  
Qing Kang ◽  
Yin Xiao ◽  
Wenping Hu ◽  
Yong Wang
Keyword(s):  
Fluorescence Tags

Smartly designed AIE triazoliums as unique targeting fluorescence tags for specific sulfonic biomacromolecule recognition via ‘electrostatic locking’.

scholarly journals Microhomology-based CRISPR tagging tools for protein tracking, purification, and depletion

2019 ◽  
Vol 294 (28) ◽  
pp. 10877-10885 ◽  
Author(s):  
Da-Wei Lin ◽  
Benjamin P. Chung ◽  
Jia-Wei Huang ◽  
Xiaorong Wang ◽  
Lan Huang ◽  
...  
Keyword(s):  
Protein Function ◽  
Mammalian Cells ◽  
Gene Locus ◽  
End Joining ◽  
High Quality ◽  
Physiological Conditions ◽  
Protein Tracking ◽  
Endogenous Proteins ◽  
And Behavior ◽  
Fluorescence Tags

Work in yeast models has benefitted tremendously from the insertion of epitope or fluorescence tags at the native gene locus to study protein function and behavior under physiological conditions. In contrast, work in mammalian cells largely relies on overexpression of tagged proteins because high-quality antibodies are only available for a fraction of the mammalian proteome. CRISPR/Cas9-mediated genome editing has recently emerged as a powerful genome-modifying tool that can also be exploited to insert various tags and fluorophores at gene loci to study the physiological behavior of proteins in most organisms, including mammals. Here we describe a versatile toolset for rapid tagging of endogenous proteins. The strategy utilizes CRISPR/Cas9 and microhomology-mediated end joining repair for efficient tagging. We provide tools to insert 3×HA, His6FLAG, His6-Biotin-TEV-RGSHis6, mCherry, GFP, and the auxin-inducible degron tag for compound-induced protein depletion. This approach and the developed tools should greatly facilitate functional analysis of proteins in their native environment.

Engineered fluorescence tags for in vivo protein labelling

RSC Advances ◽  
2014 ◽  
Vol 4 (14) ◽  
pp. 7235-7245 ◽  
Author(s):  
Zhipeng Wang ◽  
Xiaozhe Ding ◽  
Sijian Li ◽  
Jing Shi ◽  
Yiming Li
Keyword(s):  
Fluorescent Probe ◽  
Chemical Biology ◽  
Protein Distribution ◽  
Protein Labelling ◽  
Important Chemical ◽  
Probe System ◽  
And Function ◽  
Fluorescence Tags ◽  
Peptide Tag

In vivoprotein labelling with a peptide tag–fluorescent probe system is an important chemical biology strategy for studying protein distribution, interaction and function.

scholarly journals Cell uptake, intracellular distribution, fate and reactive oxygen species generation of polymer brush engineered CeO2−xNPs

Nanoscale ◽  
2015 ◽  
Vol 7 (15) ◽  
pp. 6588-6598 ◽  
Author(s):  
Yuan Qiu ◽  
Elena Rojas ◽  
Richard A. Murray ◽  
Joseba Irigoyen ◽  
Danijela Gregurec ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Surface Charge ◽  
Polymer Brush ◽  
Reactive Oxygen Species Generation ◽  
Cell Uptake ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Oxygen Species ◽  
Biological Fate ◽  
Fluorescence Tags

Cerium Oxide nanoparticles are modified with spherical brushes of charged polyelectrolytes to study the effect of the surface charge on their biological fate and to shield fluorescence tags.

Biotags Based on Surface-Enhanced Raman Can Be as Bright as Fluorescence Tags

Nano Letters ◽  
2015 ◽  
Vol 15 (10) ◽  
pp. 6745-6750 ◽  
Author(s):  
Alessia Pallaoro ◽  
Gary B. Braun ◽  
Martin Moskovits
Keyword(s):  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Fluorescence Tags

scholarly journals Non-invasive and label-free 3D-visualization shows in vivo oligomerization of the staphylococcal alkaline shock protein 23 (Asp23)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Inga Petersen ◽  
Rabea Schlüter ◽  
Katharina J. Hoff ◽  
Volkmar Liebscher ◽  
Gert Bange ◽  
...  
Keyword(s):  
Spatial Distribution ◽  
3D Visualization ◽  
Bacterial Cells ◽  
Label Free ◽  
Serial Sections ◽  
Non Invasive ◽  
Native Proteins ◽  
Fluorescence Tags

AbstractFluorescence-tags, commonly used to visualize the spatial distribution of proteins within cells, can influence the localization of the tagged proteins by affecting their stability, interaction with other proteins or the induction of oligomerization artifacts. To circumvent these obstacles, a protocol was developed to generate 50 nm thick serial sections suitable for immunogold labeling and subsequent reconstruction of the spatial distribution of immuno-labeled native proteins within individual bacterial cells. Applying this method, we show a cellular distribution of the staphylococcal alkaline shock protein 23 (Asp23), which is compatible with filament formation, a property of Asp23 that we also demonstrate in vitro.

Fluorescence tags using FRET networks and DNA structural change

2021 ◽  
Author(s):  
Yusuke Ogura ◽  
Keita Hayashi ◽  
Jun Tanida
Keyword(s):  
Structural Change ◽  
Fluorescence Tags

Carbosilane dendrimers peripherally functionalized with dansyl fluorescence tags and their cellular internalization studies

2009 ◽  
Vol 7 (15) ◽  
pp. 3079 ◽  
Author(s):  
Paula Ortega ◽  
Ma Jesús Serramía ◽  
Rafael Samaniego ◽  
F. Javier de la Mata ◽  
Rafael Gomez ◽  
...  
Keyword(s):  
Cellular Internalization ◽  
Carbosilane Dendrimers ◽  
Fluorescence Tags

scholarly journals FORTIS: a live-cell assay to monitor AMPA receptors using pH-sensitive fluorescence tags

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
María Calleja-Felipe ◽  
Magdalena Natalia Wojtas ◽  
Marta Diaz-González ◽  
Dalila Ciceri ◽  
Raúl Escribano ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Ampa Receptors ◽  
Population Level ◽  
Neuronal Population ◽  
Neuroscience Research ◽  
Genetic Modifications ◽  
Rapid Changes ◽  
Microplate Reader ◽  
Pharmacological Manipulations ◽  
Fluorescence Tags

AbstractThe real-time live fluorescent monitoring of surface AMPA receptors (AMPARs) could open new opportunities for drug discovery and phenotypic screening concerning neuropsychiatric disorders. We have developed FORTIS, a tool based on pH sensitivity capable of detecting subtle changes in surface AMPARs at a neuronal population level. The expression of SEP-GluA1 or pHuji-GluA1 recombinant AMPAR subunits in mammalian neurons cultured in 96-well plates enables surface AMPARs to be monitored with a microplate reader. Thus, FORTIS can register rapid changes in surface AMPARs induced by drugs or genetic modifications without having to rely on conventional electrophysiology or imaging. By combining FORTIS with pharmacological manipulations, basal surface AMPARs, and plasticity-like changes can be monitored. We expect that employing FORTIS to screen for changes in surface AMPARs will accelerate both neuroscience research and drug discovery.

Coexpressing shRNA with fluorescence tags for quantification of cell migration studies

2012 ◽  
Vol 39 (7) ◽  
pp. 7695-7703 ◽  
Author(s):  
Christine Xing’er Koo ◽  
Wanru Fang ◽  
Manuel Salto-Tellez ◽  
David Tai Leong
Keyword(s):  
Cell Migration ◽  
Migration Studies ◽  
Fluorescence Tags
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