A gene-activating skin substitute comprising PLLA/POSS nanofibers and plasmid DNA encoding ANG and bFGF promotes in vivo revascularization and epidermalization

2018 ◽  
Vol 6 (43) ◽  
pp. 6977-6992 ◽  
Author(s):  
Wenqiang Li ◽  
Dongwei Wu ◽  
Jianwang Tan ◽  
Zhibin Liu ◽  
Lu Lu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Sustained Release ◽  
Plasmid Dna ◽  
Nanofibrous Scaffold ◽  
Skin Substitute ◽  
Dna Encoding ◽  
Dermal Wound Healing ◽  
Plasmid Dnas ◽  
Dermal Wound

A gene-activated porous nanofibrous scaffold for effectively promoting vascularization, epidermalization and dermal wound healing by sustained release of dual plasmid DNAs.

Systemic Isotretinoin: Effects on Dermal Wound Healing in a Rabbit Ear Model In Vivo

1990 ◽  
Vol 16 (12) ◽  
pp. 1142-1146 ◽  
Author(s):  
RONALD L. MOY ◽  
LARRY S. MOY ◽  
RICHARD G. BENNETT ◽  
JOHN A. ZITELLI ◽  
JOUNI UITTO
Keyword(s):  
Wound Healing ◽  
Dermal Wound Healing ◽  
Rabbit Ear ◽  
Dermal Wound

Antioxidant, antibacterial and in vivo dermal wound healing effects of Opuntia flower extracts

2015 ◽  
Vol 81 ◽  
pp. 483-490 ◽  
Author(s):  
Imene Ammar ◽  
Sana Bardaa ◽  
Massara Mzid ◽  
Zouheir Sahnoun ◽  
Tarak Rebaii ◽  
...  
Keyword(s):  
Wound Healing ◽  
Dermal Wound Healing ◽  
Dermal Wound

scholarly journals Hypoxia Preconditioned Serum (HPS)-Hydrogel Can Accelerate Dermal Wound Healing in Mice—An In Vivo Pilot Study

Biomedicines ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 176
Author(s):  
Jun Jiang ◽  
Ursula Kraneburg ◽  
Ulf Dornseifer ◽  
Arndt F. Schilling ◽  
Ektoras Hadjipanayi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Lymphatic Vessels ◽  
Scar Tissue ◽  
Tissue Samples ◽  
Medicine Research ◽  
Dermal Wound Healing ◽  
Wound Model ◽  
Dermal Wound

The ability to use the body’s resources to promote wound repair is increasingly becoming an interesting area of regenerative medicine research. Here, we tested the effect of topical application of blood-derived hypoxia preconditioned serum (HPS) on wound healing in a murine wound model. Alginate hydrogels loaded with two different HPS concentrations (10 and 40%) were applied topically on full-thickness wounds created on the back of immunocompromised mice. We achieved a significant dose-dependent wound area reduction after 5 days in HPS-treated groups compared with no treatment (NT). On average, both HPS-10% and HPS-40% -treated wounds healed 1.4 days faster than NT. Healed tissue samples were investigated on post-operative day 15 (POD 15) by immunohistology and showed an increase in lymphatic vessels (LYVE-1) up to 45% with HPS-40% application, while at this stage, vascularization (CD31) was comparable in the HPS-treated and NT groups. Furthermore, the expression of proliferation marker Ki67 was greater on POD 15 in the NT-group compared to HPS-treated groups, in accordance with the earlier completion of wound healing observed in the latter. Collagen deposition was similar in all groups, indicating lack of scar tissue hypertrophy as a result of HPS-hydrogel treatment. These findings show that topical HPS application is safe and can accelerate dermal wound healing in mice.

scholarly journals Development of an in-situ forming, self-healing scaffold for dermal wound healing: in-vivo and in-vivo studies

2021 ◽  
pp. 112263
Author(s):  
Swati Sharma ◽  
Harishkumar Madhyastha ◽  
K. Laxmi Swetha ◽  
Kavya Sree Maravajjala ◽  
Archana Singh ◽  
...  
Keyword(s):  
Wound Healing ◽  
In Vivo Studies ◽  
Self Healing ◽  
Dermal Wound Healing ◽  
In Situ Forming ◽  
Dermal Wound

scholarly journals In vivo evaluation of an electrospun and 3D printed cellular delivery device for dermal wound healing

2020 ◽  
Vol 108 (6) ◽  
pp. 2560-2570 ◽  
Author(s):  
Ryan M. Clohessy ◽  
David J. Cohen ◽  
Karolina Stumbraite ◽  
Barbara D. Boyan ◽  
Zvi Schwartz
Keyword(s):  
Wound Healing ◽  
Cellular Delivery ◽  
Delivery Device ◽  
In Vivo Evaluation ◽  
Dermal Wound Healing ◽  
3D Printed ◽  
Dermal Wound

Fabrication, characterization and in vivo evaluation of dexpanthenol sustained-release nanofibers for wound healing

2020 ◽  
Vol 91 ◽  
pp. 106827
Author(s):  
Maryam Najafiasl ◽  
Shahriar Osfouri ◽  
Reza Azin ◽  
Sasan Zaeri
Keyword(s):  
Wound Healing ◽  
Sustained Release ◽  
In Vivo Evaluation

scholarly journals Ex vivoexpanded haematopoietic progenitor cells improve dermal wound healing by paracrine mechanisms

2009 ◽  
Vol 18 (5) ◽  
pp. 445-453 ◽  
Author(s):  
Christian Templin ◽  
Karsten Grote ◽  
Kai Schledzewski ◽  
Jelena-Rima Ghadri ◽  
Sabine Schnabel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Progenitor Cells ◽  
Dermal Wound Healing ◽  
Dermal Wound

scholarly journals Augmentation of Dermal Wound Healing by Adipose Tissue-Derived Stromal Cells (ASC)

2018 ◽  
Vol 5 (4) ◽  
pp. 91 ◽  
Author(s):  
Joris van Dongen ◽  
Martin Harmsen ◽  
Berend van der Lei ◽  
Hieronymus Stevens
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Randomized Clinical Trials ◽  
Cell Types ◽  
Matrix Remodeling ◽  
Skin Wound Healing ◽  
Dermal Wound Healing ◽  
Dermal Wound

The skin is the largest organ of the human body and is the first line of defense against physical and biological damage. Thus, the skin is equipped to self-repair and regenerates after trauma. Skin regeneration after damage comprises a tightly spatial-temporally regulated process of wound healing that involves virtually all cell types in the skin. Wound healing features five partially overlapping stages: homeostasis, inflammation, proliferation, re-epithelization, and finally resolution or fibrosis. Dysreguled wound healing may resolve in dermal scarring. Adipose tissue is long known for its suppressive influence on dermal scarring. Cultured adipose tissue-derived stromal cells (ASCs) secrete a plethora of regenerative growth factors and immune mediators that influence processes during wound healing e.g., angiogenesis, modulation of inflammation and extracellular matrix remodeling. In clinical practice, ASCs are usually administered as part of fractionated adipose tissue i.e., as part of enzymatically isolated SVF (cellular SVF), mechanically isolated SVF (tissue SVF), or as lipograft. Enzymatic isolation of SVF obtained adipose tissue results in suspension of adipocyte-free cells (cSVF) that lack intact intercellular adhesions or connections to extracellular matrix (ECM). Mechanical isolation of SVF from adipose tissue destructs the parenchyma (adipocytes), which results in a tissue SVF (tSVF) with intact connections between cells, as well as matrix. To date, due to a lack of well-designed prospective randomized clinical trials, neither cSVF, tSVF, whole adipose tissue, or cultured ASCs can be indicated as the preferred preparation procedure prior to therapeutic administration. In this review, we present and discuss current literature regarding the different administration options to apply ASCs (i.e., cultured ASCs, cSVF, tSVF, and lipografting) to augment dermal wound healing, as well as the available indications for clinical efficacy.

scholarly journals Fabrication of chitosan-polyvinyl alcohol and silk electrospun fiber seeded with differentiated keratinocyte for skin tissue regeneration in animal wound model

2020 ◽  
Author(s):  
Afshin Fathi ◽  
Mehdi Khanmohammadi ◽  
Arash Goodarzi ◽  
Lale Foroutani ◽  
Zahra Taherian Mobarakeh ◽  
...  
Keyword(s):  
Wound Healing ◽  
Polyvinyl Alcohol ◽  
Electrospun Fiber ◽  
Biochemical Properties ◽  
Skin Substitute ◽  
Natural Polymers ◽  
Silk Fibers ◽  
Wound Model

Abstract Hybrid fibrous mat containing cell interactive molecules offers the ability to deliver the cells and drugs in wound bed, which will help to achieve a high therapeutic treatment. In this study, a co-electrospun hybrid of polyvinyl alcohol (PVA), chitosan (Ch) and silk fibrous mat was developed and their wound healing potential by localizing bone marrow mesenchymal stem cells (MSCs)-derived keratinocytes on it was evaluated in vitro and in vivo. It was expected that fabricated hybrid construct could promote wound healing due to its structure, physical, biological specifications. The fabricated fibrous mats were characterized for their structural, mechanical and biochemical properties. The shape uniformity and pore size of fibers showed smooth and homogenous structures of them. Fourier transform infrared spectroscopy (FTIR) verified all typical absorption characteristics of Ch-PVA + Silk polymers as well as Ch-PVA or pure PVA substrates. The contact angle and wettability measurement of fibers showed that mats found moderate hydrophilicity by addition of Ch and silk substrates compared with PVA alone. The mechanical features of Ch-PVA + Silk fibrous mat increase significantly through co-electrospun process as well as hybridization of these synthetic and natural polymers. Higher degrees of cellular attachment and proliferation obtained on Ch-PVA + Silk fibers compared with PVA and Ch-PVA fibers. In terms of the capability of Ch-PVA + Silk fibers and MSC-derived keratinocytes, histological analysis and skin regeneration results showed this novel fibrous construct could be suggested as a skin substitute in the repair of injured skin and regenerative medicine applications.

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