Mammalian cells: a unique scaffold forin situbiosynthesis of metallic nanomaterials and biomedical applications

2018 ◽  
Vol 6 (41) ◽  
pp. 6501-6514 ◽  
Author(s):  
Fawad Ur Rehman ◽  
Hui Jiang ◽  
Matthias Selke ◽  
Xuemei Wang
Keyword(s):  
Mammalian Cells ◽  
Biomedical Applications ◽  
Nanoscale Materials ◽  
Metallic Nanomaterials

Nanoscale materials biosynthesis by using mammalian scaffold is green and highly biocompatible.

Bifunctionally engineered polyethylenimines as efficient DNA carriers and antibacterials against resistant pathogens

2018 ◽  
Vol 33 (3) ◽  
pp. 363-379
Author(s):  
Z Ahmadi ◽  
D Jha ◽  
B Kumar ◽  
HK Gautam ◽  
Pradeep Kumar
Keyword(s):  
Gel Electrophoresis ◽  
Mammalian Cells ◽  
Biomedical Applications ◽  
Pathogenic Bacteria ◽  
Low Molecular Weight ◽  
High Cell ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Resistant Strains ◽  
Better Than

In this study, we have designed and developed two series of bifunctional conjugates by tethering polyethylenimine with streptomycin. By varying the amount of streptomycin, conjugates, polyethylenimine-streptomycin, have been synthesized and characterized spectroscopically. Gel electrophoresis assay revealed a slight decrease in the cationic charge density on the conjugates as these retarded the mobility of pDNA at higher w/w ratios. Further, transfection studies showed that both the series of conjugates transfected the mammalian cells efficiently with low-molecular weight polyethylenimine-streptomycin conjugates were more competent (∼9-fold enhancement with respect to native bPEI) exhibiting high cell viability too. Besides, both the series of conjugates displayed excellent antibacterial activity on pathogenic bacteria, even better than native streptomycin on resistant strains. Altogether, these results ensure the promising potential of the projected bifunctional conjugates as safe and efficient gene delivery vectors as well as antibacterials for future biomedical applications.

scholarly journals Bioprospection of Natural Sources of Polyphenols with Therapeutic Potential for Redox-Related Diseases

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 789 ◽  
Author(s):  
Regina Menezes ◽  
Alexandre Foito ◽  
Carolina Jardim ◽  
Inês Costa ◽  
Gonçalo Garcia ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biomedical Applications ◽  
Therapeutic Potential ◽  
Integrative Approach ◽  
Natural Sources ◽  
Analysis And Evaluation ◽  
Cellular Assays ◽  
Health Promoting ◽  
Rubus Species ◽  
Anti Inflammatory

Plants are a reservoir of high-value molecules with underexplored biomedical applications. With the aim of identifying novel health-promoting attributes in underexplored natural sources, we scrutinized the diversity of (poly)phenols present within the berries of selected germplasm from cultivated, wild, and underutilized Rubus species. Our strategy combined the application of metabolomics, statistical analysis, and evaluation of (poly)phenols’ bioactivity using a yeast-based discovery platform. We identified species as sources of (poly)phenols interfering with pathological processes associated with redox-related diseases, particularly, amyotrophic lateral sclerosis, cancer, and inflammation. In silico prediction of putative bioactives suggested cyanidin–hexoside as an anti-inflammatory molecule which was validated in yeast and mammalian cells. Moreover, cellular assays revealed that the cyanidin moiety was responsible for the anti-inflammatory properties of cyanidin–hexoside. Our findings unveiled novel (poly)phenolic bioactivities and illustrated the power of our integrative approach for the identification of dietary (poly)phenols with potential biomedical applications.

scholarly journals Mucoadhesive Hydrogel Nanoparticles as Smart Biomedical Drug Delivery System

2019 ◽  
Vol 9 (5) ◽  
pp. 825 ◽  
Author(s):  
Nemany Hanafy ◽  
Stefano Leporatti ◽  
Maged El-Kemary
Keyword(s):  
Drug Delivery ◽  
Biological Activity ◽  
Mammalian Cells ◽  
Biomedical Applications ◽  
Biological Fluids ◽  
Medical Applications ◽  
Stimuli Responsive ◽  
Stimuli Responsive Polymers ◽  
Responsive Polymers ◽  
Hydrogel Formulation

Hydrogels are widely used materials which have many medical applications. Their ability to absorb aqueous solutions and biological fluids gives them innovative characterizations resulting in increased compatibility with biological activity. In this sense, they are used extensively for encapsulation of several targets such as biomolecules, viruses, bacteria, and mammalian cells. Indeed, many methods have been published which are used in hydrogel formulation and biomedical encapsulations involving several cross-linkers. This system is still rich with the potential of undiscovered features. The physicochemical properties of polymers, distinguished by their interactions with biological systems into mucoadhesive, gastro-adhesive, and stimuli responsive polymers. Hydrogel systems may be assembled as tablets, patches, gels, ointments, and films. Their potential to be co-formulated as nanoparticles extends the limits of their assembly and application. In this review, mucoadhesive nanoparticles and their importance for biomedical applications are highlighted with a focus on mechanisms of overcoming mucosal resistance.

scholarly journals Transport and programmed release of nanoscale cargo from cells by using NETosis

Nanoscale ◽  
2020 ◽  
Vol 12 (16) ◽  
pp. 9104-9115 ◽  
Author(s):  
Daniel Meyer ◽  
Saba Telele ◽  
Anna Zelená ◽  
Alice J. Gillen ◽  
Alessandra Antonucci ◽  
...  
Keyword(s):  
Immune Cells ◽  
Biomedical Applications ◽  
Nanoscale Materials ◽  
Cellular Functions ◽  
Programmed Release

Immune cells take up nanoscale materials and can be programmed to release it again, which has important implications for understanding cellular functions, biocompatibility as well as biomedical applications.

Carbon Nano-onions: A Valuable Class of Carbon Nanomaterials in Biomedicine

2019 ◽  
Vol 26 (38) ◽  
pp. 6915-6929 ◽  
Author(s):  
Silvia Giordani ◽  
Adalberto Camisasca ◽  
Viviana Maffeis
Keyword(s):  
Quality Of Life ◽  
Biomedical Applications ◽  
Carbon Nanomaterials ◽  
Nanoscale Materials ◽  
Improve Quality ◽  
Good Biocompatibility

: The development of nanoscale materials is an important area of research as it provides access to materials with unique properties that can be applied to improve quality of life. Multi-layer fullerenes, also known as carbon nano-onions (CNOs) are an exciting class of nanostructures which show great versatility and applicability. They find applications in several fields of technology and biomedicine. This review highlights the potential advantages of CNOs for biomedical applications, which include but are not limited to bioimaging and sensing. Their good biocompatibility renders them promising platforms for the development of novel healthcare devices.

Biomimetic Principles to Develop Blood Compatible Surfaces

2017 ◽  
Vol 40 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Vladislav Semak ◽  
Michael B. Fischer ◽  
Viktoria Weber
Keyword(s):  
Surface Functionalization ◽  
Mammalian Cells ◽  
Membrane Structure ◽  
Biomedical Applications ◽  
Nonspecific Adsorption ◽  
Biomaterial Surface ◽  
Biomaterial Surfaces ◽  
Biomedical Technologies ◽  
Surface Patterns ◽  
Over Time

Functionalized biomaterial surface patterns capable of resisting nonspecific adsorption while retaining their bioactivity are crucial in the advancement of biomedical technologies, but currently available biomaterials intended for use in whole blood frequently suffer from nonspecific adsorption of proteins and cells, leading to a loss of activity over time. In this review, we address two concepts for the design and modification of blood compatible biomaterial surfaces, zwitterionic modification and surface functionalization with glycans – both of which are inspired by the membrane structure of mammalian cells – and discuss their potential for biomedical applications.

Synthesis Fe-Ni Alloy Magnetic Nanoparticles for Biomedical Applications

2006 ◽  
Author(s):  
Ming-Fong Tai ◽  
Jong-Kai Hsiao ◽  
Hon-Man Liu ◽  
Shio-Chao Lee ◽  
Shin-Tai Chen
Keyword(s):  
Magnetic Nanoparticles ◽  
Saturation Magnetization ◽  
Mammalian Cells ◽  
Biomedical Applications ◽  
Magnetic Beads ◽  
Combustion Method ◽  
Chemical Precipitation ◽  
Mri Contrast Agent ◽  
Precipitation Method

In this investigation, we synthesize FeNi alloy magnetic nanoparticles (MNPs) by using both chemical precipitation and combustion methods. The FeNi MNPs prepared by combustion method have a rather high saturation magnetization Ms of ∼180 emu/g and a coercivity field Hc of near zero. The functionalized FeNi MNPs which were coated with biocompatible polyethyleneimine (PEI) polymer have also been synthesized. We demonstrated that the PEI coated FeNi MNPs can enter the mammalian cells in vitro and can be used as a magnetic resonance imagine (MRI) contrast agent. The results demonstrated that FeNi MNPs potentially could be applied in the biomedical field. To prepare a higher quality and well controlled Fe-Ni MNPs, we also developed a thermal reflux chemical precipitation method to synthesize FeNi3 alloy MNPs. The precursor chemicals of Fe(acac)3 and Ni(acac)2 in a molecular ratio of 1:3 reacted in octyl ether solvent at the boiling point of solvent (∼300°C) by the thermal reflux process. The 1,2-hexadecandiol and tri-n-octylphosphine oxide (TOPO) were used as reducer and surfactant, respectively. The chemically precipitated FeNi3 MNPs are well dispensed and have well-controlled particle sizes around 10–20 nm with a very narrow size distribution (± 1.2 nm). The highly monodispersive FeNi3 NPs present good uniformity in particle shape and crystallinity on particle surfaces. The MNPs exhibit well soft magnetism with saturation magnetization of ∼61 emu/g and Hc ∼ 0. The functionalized magnetic beads with biocompatible polymer coated on MNPs are also generated completed for biomedical applications.

scholarly journals Biomedical Applications of Nanomaterials: Diagnosis and Therapy of Thrombotic Disorders

2017 ◽  
Vol 53 (01) ◽  
pp. 036-040
Author(s):  
D. Dash
Keyword(s):  
Gold Nanorods ◽  
Photothermal Therapy ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Infrared Laser ◽  
Coronary Risk ◽  
Innovative Strategy ◽  
Diagnosis And Therapy ◽  
Metallic Nanomaterials

ABSTRACTWe have employed unique properties of carbon-based as well as metallic nanomaterials to develop diagnostic / therapeutic devices targeted against thrombotic disorders. We have designed a novel graphene-based biosensor that can detect individuals with high coronary risk. Further, we describe an innovative strategy to ablate pathological thrombus in situ employing near-infrared laser-irradiated gold nanorods (photothermal therapy).

scholarly journals Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability

Materials ◽  
2019 ◽  
Vol 12 (6) ◽  
pp. 949 ◽  
Author(s):  
Xuemei Xu ◽  
Shiqi Fang ◽  
Yuan Zhuang ◽  
Shanshan Wu ◽  
Qingling Pan ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biomedical Applications ◽  
Transfection Efficiency ◽  
Dna Origami ◽  
Biological Applications ◽  
Dna Nanostructures ◽  
Cationic Protein ◽  
Physiological Conditions ◽  
Cellular Transfection ◽  
Biological Environment

DNA nanostructures, owing to their controllable and adaptable nature, have been considered as highly attractive nanoplatforms for biomedical applications in recent years. However, their use in the biological environment has been restricted by low cellular transfection efficiency in mammalian cells, weak stability under physiological conditions, and endonuclease degradation. Herein, we demonstrate an effective approach to facilitate fast transfection of DNA nanostructures and enhance their stability by encapsulating DNA origami with a biocompatible cationic protein (cHSA) via electrostatic interaction. The coated DNA origami is found to be stable under physiological conditions. Moreover, the cHSA coating could significantly improve the cellular transfection efficiency of DNA origami, which is essential for biological applications.

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