Biomimetic Principles to Develop Blood Compatible Surfaces

2017 ◽  
Vol 40 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Vladislav Semak ◽  
Michael B. Fischer ◽  
Viktoria Weber
Keyword(s):  
Surface Functionalization ◽  
Mammalian Cells ◽  
Membrane Structure ◽  
Biomedical Applications ◽  
Nonspecific Adsorption ◽  
Biomaterial Surface ◽  
Biomaterial Surfaces ◽  
Biomedical Technologies ◽  
Surface Patterns ◽  
Over Time

Functionalized biomaterial surface patterns capable of resisting nonspecific adsorption while retaining their bioactivity are crucial in the advancement of biomedical technologies, but currently available biomaterials intended for use in whole blood frequently suffer from nonspecific adsorption of proteins and cells, leading to a loss of activity over time. In this review, we address two concepts for the design and modification of blood compatible biomaterial surfaces, zwitterionic modification and surface functionalization with glycans – both of which are inspired by the membrane structure of mammalian cells – and discuss their potential for biomedical applications.

Bifunctionally engineered polyethylenimines as efficient DNA carriers and antibacterials against resistant pathogens

2018 ◽  
Vol 33 (3) ◽  
pp. 363-379
Author(s):  
Z Ahmadi ◽  
D Jha ◽  
B Kumar ◽  
HK Gautam ◽  
Pradeep Kumar
Keyword(s):  
Gel Electrophoresis ◽  
Mammalian Cells ◽  
Biomedical Applications ◽  
Pathogenic Bacteria ◽  
Low Molecular Weight ◽  
High Cell ◽  
Gene Delivery Vectors ◽  
Efficient Gene ◽  
Resistant Strains ◽  
Better Than

In this study, we have designed and developed two series of bifunctional conjugates by tethering polyethylenimine with streptomycin. By varying the amount of streptomycin, conjugates, polyethylenimine-streptomycin, have been synthesized and characterized spectroscopically. Gel electrophoresis assay revealed a slight decrease in the cationic charge density on the conjugates as these retarded the mobility of pDNA at higher w/w ratios. Further, transfection studies showed that both the series of conjugates transfected the mammalian cells efficiently with low-molecular weight polyethylenimine-streptomycin conjugates were more competent (∼9-fold enhancement with respect to native bPEI) exhibiting high cell viability too. Besides, both the series of conjugates displayed excellent antibacterial activity on pathogenic bacteria, even better than native streptomycin on resistant strains. Altogether, these results ensure the promising potential of the projected bifunctional conjugates as safe and efficient gene delivery vectors as well as antibacterials for future biomedical applications.

Superparamagnetic Twist-Type Actuators with Shape-Independent Magnetic Properties and Surface Functionalization for Advanced Biomedical Applications

2014 ◽  
Vol 24 (33) ◽  
pp. 5269-5276 ◽  
Author(s):  
Christian Peters ◽  
Olgaç Ergeneman ◽  
Pedro D. Wendel García ◽  
Michelle Müller ◽  
Salvador Pané ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Surface Functionalization ◽  
Biomedical Applications

Calcium Orthophosphate Bioceramics

2010 ◽  
Vol 5 ◽  
pp. 57-100 ◽  
Author(s):  
Sergey V. Dorozhkin
Keyword(s):  
Biomedical Applications ◽  
Skeletal Structure ◽  
Calcium Orthophosphate ◽  
Skeletal System ◽  
The Past ◽  
Calcium Orthophosphates ◽  
Initial Work ◽  
Compositional Control ◽  
Over Time ◽  
Made In

Ceramics used for the repair and reconstruction of diseased or damaged parts of the musculo-skeletal system, termed bioceramics, can be bioinert, bioresorbable and bioactive, as well as porous for tissue ingrowth. This review is devoted to calcium orthophosphates, which belong to the categories of bioresorbable and bioactive bioceramics. There have been a number of major advances made in this field during the past 30 – 40 years. From initial work on development of bioceramics that were tolerated in the physiological environment, emphasis has now shifted towards the use of bioceramics that interact with bone tissue by forming a direct bond. By structural and compositional control, it is now possible to choose whether the bioceramics of calcium orthophosphates are biologically stable once incorporated within the skeletal structure or whether they are resorbed over time. Current biomedical applications of calcium orthophosphate bioceramics include replacements for hips, knees, teeth, tendons and ligaments, as well as repair for periodontal disease, maxillofacial reconstruction, augmentation and stabilization of the jawbone, spinal fusion and bone fillers after tumor surgery.

scholarly journals Biomedical Applications: Surface Functionalization of Gold Nanoparticles with Red Blood Cell Membranes (Adv. Mater. 26/2013)

2013 ◽  
Vol 25 (26) ◽  
pp. 3616-3616 ◽  
Author(s):  
Weiwei Gao ◽  
Che-Ming J. Hu ◽  
Ronnie H. Fang ◽  
Brian T. Luk ◽  
Jing Su ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Blood Cell ◽  
Surface Functionalization ◽  
Red Blood Cell ◽  
Biomedical Applications ◽  
Cell Membranes ◽  
Red Blood Cell Membranes

scholarly journals The Smart Solution for DNA Removal in Biopharmaceutical Production by Benzonase Endonuclease

2013 ◽  
Vol 2 (1) ◽  
Author(s):  
Zhu Yang ◽  
Mingxin Li ◽  
Wen Chen ◽  
Alexander Peters
Keyword(s):  
Mammalian Cells ◽  
Rna World ◽  
Rapid Development ◽  
World Health ◽  
Base Pairs ◽  
Biological Products ◽  
Continuous Cell Lines ◽  
Dna And Rna ◽  
Biomedical Technologies ◽  
Health Organization

<p>More and more mammalian cells especially continuous cell lines were used to produce vaccines and therapeutic biological products with the rapid development of biomedical technologies. Therefore, more attention should be paid from drug regulatory authorities to the safety of biological products especially quality control of residual DNA and RNA. World Health Organization has evaluated the methods commonly used in vaccine manufacture for their ability to reduce the biological activity of DNA. Benzonase endonuclease is frequently used during the production of vaccines and can degrade all nucleic acid sequences down to oligonucleotides of approximately 3 to 5 base pairs.</p>

scholarly journals Bioprospection of Natural Sources of Polyphenols with Therapeutic Potential for Redox-Related Diseases

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 789 ◽  
Author(s):  
Regina Menezes ◽  
Alexandre Foito ◽  
Carolina Jardim ◽  
Inês Costa ◽  
Gonçalo Garcia ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biomedical Applications ◽  
Therapeutic Potential ◽  
Integrative Approach ◽  
Natural Sources ◽  
Analysis And Evaluation ◽  
Cellular Assays ◽  
Health Promoting ◽  
Rubus Species ◽  
Anti Inflammatory

Plants are a reservoir of high-value molecules with underexplored biomedical applications. With the aim of identifying novel health-promoting attributes in underexplored natural sources, we scrutinized the diversity of (poly)phenols present within the berries of selected germplasm from cultivated, wild, and underutilized Rubus species. Our strategy combined the application of metabolomics, statistical analysis, and evaluation of (poly)phenols’ bioactivity using a yeast-based discovery platform. We identified species as sources of (poly)phenols interfering with pathological processes associated with redox-related diseases, particularly, amyotrophic lateral sclerosis, cancer, and inflammation. In silico prediction of putative bioactives suggested cyanidin–hexoside as an anti-inflammatory molecule which was validated in yeast and mammalian cells. Moreover, cellular assays revealed that the cyanidin moiety was responsible for the anti-inflammatory properties of cyanidin–hexoside. Our findings unveiled novel (poly)phenolic bioactivities and illustrated the power of our integrative approach for the identification of dietary (poly)phenols with potential biomedical applications.

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