Multiple microarrays of non-adherent cells on a single 3D stimuli-responsive binary polymer-brush pattern

2018 ◽  
Vol 6 (29) ◽  
pp. 4792-4798 ◽  
Author(s):  
Jianwen Hou ◽  
Runhai Chen ◽  
Jingchuan Liu ◽  
Haozheng Wang ◽  
Qiang Shi ◽  
...  
Keyword(s):  
Polymer Brush ◽  
Adherent Cells ◽  
Stimuli Responsive ◽  
Con A ◽  
Binary Polymer ◽  
Cell Microarrays ◽  
Single Pattern ◽  
Multiple Cell

A hierarchically binary PGAMA/PNIPAM pattern is fabricated, and multiple cell microarrays are formed on this single pattern with the aid of Con A and temperature.

scholarly journals The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes: II. Nature of the responding cells.

1977 ◽  
Vol 145 (1) ◽  
pp. 163-174 ◽  
Author(s):  
A S Kong ◽  
S I Morse
Keyword(s):  
T Cells ◽  
Bordetella Pertussis ◽  
T Cell Response ◽  
Adherent Cells ◽  
Phagocytic Cells ◽  
Mitogenic Response ◽  
Con A ◽  
Surface Receptors ◽  
In Vitro Effects

The mitogenic response of murine lymphocytes to the lymphocytosis-promoting factor of Bordetella pertussis has been shown to be due to activation of T cells. The selectivity of responsiveness to LPF with respect to the population of T cells which is stimllated, differs from that of PHA as well as Con A, and the surface receptors are different. A population of adherent cells, which does not appear to consist of macrophages or other phagocytic cells, is required for the T-cell response.

Facile Approach to Patterned Binary Polymer Brush through Photolithography and Surface-Initiated Photopolymerization

2010 ◽  
Vol 2 (4) ◽  
pp. 1200-1205 ◽  
Author(s):  
Xinyan Jia ◽  
Xuesong Jiang ◽  
Rui Liu ◽  
Jie Yin
Keyword(s):  
Polymer Brush ◽  
Binary Polymer

A switchable polymer brush system for antifouling and controlled detection

2017 ◽  
Vol 53 (26) ◽  
pp. 3713-3716 ◽  
Author(s):  
Serkan Demirci ◽  
Selin Kinali-Demirci ◽  
Shan Jiang
Keyword(s):  
Functional Groups ◽  
Polymer Brush ◽  
Stimuli Responsive ◽  
Surface Functionality ◽  
Responsive Polymer ◽  
Stimuli Responsive Polymer

A stimuli-responsive polymer brush system designed to switch on and off surface functionality and prevent functional groups from fouling.

scholarly journals POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS

1972 ◽  
Vol 136 (1) ◽  
pp. 143-155 ◽  
Author(s):  
Igal Gery ◽  
Byron H. Waksman
Keyword(s):  
T Cells ◽  
Bone Marrow Cells ◽  
Mouse Spleen ◽  
Adherent Cells ◽  
Spleen Cells ◽  
Con A ◽  
Cell Responses ◽  
Donor Cells ◽  
Peripheral Leukocytes ◽  
Nonadherent Cells

Effective supernatants (SUP), which potentiate mouse T-cell responses to phytohemagglutin (PHA), are obtained from cells of several species (human, rabbit, rat, mouse) and indeed from syngeneic spleen, thymus, or bone marrow cells. Unstimulated cells release some SUP activity but more is produced after stimulation. Lipopolysaccharide (LPS) produced very active SUP in all cultures tested. PHA was similarly active on human leukocytes only, whereas concanavalin A (Con A) gave highly efficient SUP only with mouse spleen cells. SUP production is not correlated with a mitotic response of the donor cells and is observed in cultures unable to respond mitotically to the stimulant. Adherent mouse spleen cell populations, consisting largely or entirely of macrophages, produce active SUP, while nonadherent cells do not. Similarly, purification of human peripheral leukocytes on nylon columns, with removal of macrophages and other adherent cells, destroys their ability to produce SUP. The importance of indirect effects in stimulating mitotic responses of T cells is emphasized by the fact that the mitotic response of mouse thymocytes to LPS and its ability to potentiate the response of these cells to PHA disappears with removal of adherent cells from the thymocyte population. Conversely the production of SUP from spleen cells stimulated by Con A requires the presence of T cells.

scholarly journals Multiscale Models in the Biomechanics of Plant Growth

Physiology ◽  
2015 ◽  
Vol 30 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Oliver E. Jensen ◽  
John A. Fozard
Keyword(s):  
Plant Physiology ◽  
Plant Growth ◽  
Cell Walls ◽  
Adherent Cells ◽  
Multiscale Models ◽  
Multiple Cell

Plant growth occurs through the coordinated expansion of tightly adherent cells, driven by regulated softening of cell walls. It is an intrinsically multiscale process, with the integrated properties of multiple cell walls shaping the whole tissue. Multiscale models encode physical relationships to bring new understanding to plant physiology and development.

scholarly journals Micrometre and nanometre scale patterning of binary polymer brushes, supported lipid bilayers and proteins

2017 ◽  
Vol 8 (6) ◽  
pp. 4517-4526 ◽  
Author(s):  
Alexander Johnson ◽  
Jeppe Madsen ◽  
Paul Chapman ◽  
Abdullah Alswieleh ◽  
Omed Al-Jaf ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Polymer Brushes ◽  
Polymer Brush ◽  
Protecting Groups ◽  
Supported Lipid Bilayers ◽  
Binary Polymer ◽  
Binary Polymer Brushes

Binary polymer brush patterns were fabricated using aminosilanes with photo-cleavable protecting groups.

Stimuli-Responsive Biocompatible Polymer Brush

Synfacts ◽  
2006 ◽  
Vol 2006 (10) ◽  
pp. 1015-1015
Author(s):  
M. Stenzel ◽  
L. Zhang ◽  
W. Huck
Keyword(s):  
Polymer Brush ◽  
Stimuli Responsive ◽  
Biocompatible Polymer

Spin-Casting Polymer Brush Films for Stimuli-Responsive and Anti-Fouling Surfaces

2016 ◽  
Vol 8 (10) ◽  
pp. 6685-6692 ◽  
Author(s):  
Binbin Xu ◽  
Chun Feng ◽  
Jianhua Hu ◽  
Ping Shi ◽  
Guangxin Gu ◽  
...  
Keyword(s):  
Polymer Brush ◽  
Stimuli Responsive ◽  
Spin Casting
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