Real-time tracking of the autophagy process in living cells using plasmonically enhanced Raman spectroscopy of fucoidan-coated gold nanoparticles

2018 ◽  
Vol 6 (34) ◽  
pp. 5460-5465 ◽  
Author(s):  
Hongje Jang ◽  
Kyungtae Kang ◽  
Mostafa A. El-Sayed
Keyword(s):  
Gold Nanoparticles ◽  
Raman Spectroscopy ◽  
Real Time ◽  
Gold Nanoparticle ◽  
Molecular Level ◽  
Living Cells ◽  
Biological Event ◽  
Real Time Tracking

Fucoidan-coated gold nanoparticle mediated autophagy triggering and PERS monitoring provide molecular level insight on a biological event.

scholarly journals Nitrone Modified Gold Nanoparticles: Synthesis, Characterization and Their Potential as 18F-Labeled PET Probes via I-SPANC

2019 ◽  
Author(s):  
Sara Ghiassian ◽  
Lihai Yu ◽  
Pierangelo Gobbo ◽  
Ali nazemi ◽  
Tommaso Romagnoli ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nmr Spectroscopy ◽  
Gold Nanoparticle ◽  
Molecular Level ◽  
Prosthetic Group ◽  
H Nmr ◽  
Nanoparticles Synthesis ◽  
Interfacial Strain ◽  
Pet Probes ◽  
High Degree

A bioorthogonal gold nanoparticle template displaying interfacial nitrone functional groups for bioorthogonal interfacial strain-promoted alkyne-nitrone cycloaddition (I-SPANC) reactions has been synthesized. The Nitrone-AuNPs were characterized in detail using <sup>1</sup>H NMR spectroscopy, TEM, TGA, and XPS and a nanoparticle raw formula was calculated. The ability to control the conjugation of molecules of interest at the molecular level onto the Nitrone-AuNPs template allowed us to create a methodology for the synthesis of AuNP-based radiolabeled probes with a high degree of loading using copper free, strained-promoted cycloaddition. To this end, we also describe the synthesis of a new prosthetic group containing a strained-alkyne capable of clicking hot <sup>18</sup>F-label onto complementary azide or nitrone labelled agents.

scholarly journals Real-Time Tracking of Lanthanide Ion Doped Upconverting Nanoparticles in Living Cells

2012 ◽  
Vol 102 (3) ◽  
pp. 200a
Author(s):  
Kang Taek Lee ◽  
Sang Hwan Nam ◽  
Yun Mi Bae ◽  
Yong Il Park ◽  
Jeong Hyun Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Living Cells ◽  
Lanthanide Ion ◽  
Real Time Tracking

scholarly journals Temperature monitoring during light-induced release of cargo using polymer capsules modified with gold nanoparticles and nanodiamonds

2021 ◽  
Vol 2015 (1) ◽  
pp. 012045
Author(s):  
E N Gerasimova ◽  
V V Yaroshenko ◽  
P M Talianov ◽  
O O Peltek ◽  
L V Mikhailova ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gold Nanoparticles ◽  
Side Effects ◽  
Temperature Measurement ◽  
Real Time ◽  
Au Nanoparticles ◽  
Living Cells ◽  
Temperature Monitoring ◽  
Biological Objects ◽  
Multifunctional Platform

Abstract Application of different light-sensitive drug delivery carriers is limited due to a risk of overheating of living cells. Therefore, a real-time temperature monitoring within biological objects that controls the photothermal release of different cargos from light-sensitive carriers is highly demanded. In this work, we develop a multifunctional platform comprised of polymer microcapsules modified with nitrogen vacancies (NV) centers as nanothermometers and gold (Au) nanoparticles (NPs) as heating elements for the realization of laser-induced cargo release with a simultaneous temperature measurement inside cells. Such platform allows to prevent unwanted side effects related with the overheating of living cells and tissues.

scholarly journals Nitrone Modified Gold Nanoparticles: Synthesis, Characterization and Their Potential as 18F-Labeled PET Probes via I-SPANC

2019 ◽  
Author(s):  
Sara Ghiassian ◽  
Lihai Yu ◽  
Pierangelo Gobbo ◽  
Ali nazemi ◽  
Tommaso Romagnoli ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Nmr Spectroscopy ◽  
Gold Nanoparticle ◽  
Molecular Level ◽  
Prosthetic Group ◽  
H Nmr ◽  
Nanoparticles Synthesis ◽  
Interfacial Strain ◽  
Pet Probes ◽  
High Degree

A bioorthogonal gold nanoparticle template displaying interfacial nitrone functional groups for bioorthogonal interfacial strain-promoted alkyne-nitrone cycloaddition (I-SPANC) reactions has been synthesized. The Nitrone-AuNPs were characterized in detail using <sup>1</sup>H NMR spectroscopy, TEM, TGA, and XPS and a nanoparticle raw formula was calculated. The ability to control the conjugation of molecules of interest at the molecular level onto the Nitrone-AuNPs template allowed us to create a methodology for the synthesis of AuNP-based radiolabeled probes with a high degree of loading using copper free, strained-promoted cycloaddition. To this end, we also describe the synthesis of a new prosthetic group containing a strained-alkyne capable of clicking hot <sup>18</sup>F-label onto complementary azide or nitrone labelled agents.

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