scholarly journals Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis

2018 ◽  
Vol 9 (30) ◽  
pp. 6340-6347 ◽  
Author(s):  
Penghui Cheng ◽  
Jianjian Zhang ◽  
Jiaguo Huang ◽  
Qingqing Miao ◽  
Chenjie Xu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Near Infrared ◽  
Reactive Oxygen ◽  
Molecular Probes ◽  
Fluorescence Probes ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Near Infrared Fluorescence

Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of keloid diseases.

A reversible near-infrared fluorescence probe for reactive oxygen species based on Te–rhodamine

2012 ◽  
Vol 48 (25) ◽  
pp. 3091 ◽  
Author(s):  
Yuichiro Koide ◽  
Mitsuyasu Kawaguchi ◽  
Yasuteru Urano ◽  
Kenjiro Hanaoka ◽  
Toru Komatsu ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Near Infrared ◽  
Fluorescence Probe ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Near Infrared Fluorescence

scholarly journals Detection of Vascular Reactive Oxygen Species in Experimental Atherosclerosis by High-Resolution Near-Infrared Fluorescence Imaging Using VCAM-1-Targeted Liposomes Entrapping a Fluorogenic Redox-Sensitive Probe

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Simona-Adriana Manea ◽  
Mihaela-Loredana Vlad ◽  
Daniela Rebleanu ◽  
Alexandra-Gela Lazar ◽  
Ioana Madalina Fenyo ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
High Resolution ◽  
Near Infrared ◽  
Reactive Oxygen ◽  
Experimental Models ◽  
Fluorescent Imaging ◽  
Quantitative Detection ◽  
Oxygen Species ◽  
Near Infrared Fluorescence ◽  
Models Of Disease

Excessive production of reactive oxygen species (ROS) and the ensuing oxidative stress are instrumental in all phases of atherosclerosis. Despite the major achievements in understanding the regulatory pathways and molecular sources of ROS in the vasculature, the specific detection and quantification of ROS in experimental models of disease remain a challenge. We aimed to develop a reliable and straightforward imaging procedure to interrogate the ROS overproduction in the vasculature and in various organs/tissues in atherosclerosis. To this purpose, the cell-impermeant ROS Brite™ 700 (RB700) probe that produces bright near-infrared fluorescence upon ROS oxidation was encapsulated into VCAM-1-targeted, sterically stabilized liposomes (VLp). Cultured human endothelial cells (EC) and macrophages (Mac) were used for in vitro experiments. C57BL6/J and ApoE-/- mice were randomized to receive normal or high-fat, cholesterol-rich diet for 10 or 32 weeks. The mice received a retroorbital injection with fluorescent tagged VLp incorporating RB700 (VLp-RB700). After two hours, the specific signals of the oxidized RB700 and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-DSPE), inserted into liposome bilayers, were measured ex vivo in the mouse aorta and various organs by high-resolution fluorescent imaging. VLp-RB700 was efficiently taken up by cultured human EC and Mac, as confirmed by fluorescence microscopy and spectrofluorimetry. After systemic administration in atherosclerotic ApoE-/- mice, VLp-RB700 were efficiently concentrated at the sites of aortic lesions, as indicated by the augmented NBD fluorescence. Significant increases in oxidized RB700 signal were detected in the aorta and in the liver and kidney of atherosclerotic ApoE-/- mice. RB700 encapsulation into sterically stabilized VCAM-1-sensitive Lp could be a novel strategy for the qualitative and quantitative detection of ROS in the vasculature and various organs and tissues in animal models of disease. The accurate and precise detection of ROS in experimental models of disease could ease the translation of the results to human pathologies.

Plasmonic Enhancement of Nitric Oxide Generation

Nanoscale ◽  
2021 ◽  
Author(s):  
Rachael Knoblauch ◽  
Chris Geddes
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Reactive Nitrogen Species ◽  
Reactive Oxygen ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Plasmonic Enhancement ◽  
Cancer Treatments

While the utility of reactive oxygen species in photodynamic therapies for both cancer treatments and antimicrobial applications has received much attention, the inherent potential of reactive nitrogen species (RNS) including...

scholarly journals A Generator of Peroxynitrite Activatable with Red Light

2021 ◽  
Author(s):  
Cristina Parisi ◽  
Mariacristina Failla ◽  
Aurore Fraix ◽  
Luca Menilli ◽  
Francesca Moret ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Nitrogen Species ◽  
Red Light ◽  
Reactive Oxygen ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
New Horizons ◽  
Light Stimuli ◽  
Spatiotemporal Control

The generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) as “unconventional” therapeutics with precise spatiotemporal control by using light stimuli may open entirely new horizons for innovative...

scholarly journals What Did We Accomplish in Fighting Radical Species in Human Health?

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Rachid Skouta
Keyword(s):  
Reactive Oxygen Species ◽  
Human Health ◽  
Reactive Nitrogen Species ◽  
Reactive Oxygen ◽  
The Body ◽  
Reactive Nitrogen ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Radical Species ◽  
Physiological Level

Maintaining the physiological level of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the body is highly important in the fight against radical species in the context of human health [...]

scholarly journals Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Micol Falabella ◽  
Elena Forte ◽  
Maria Chiara Magnifico ◽  
Paolo Santini ◽  
Marzia Arese ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Optic Neuropathy ◽  
Nitrosative Stress ◽  
Mononuclear Cells ◽  
Reactive Oxygen ◽  
Leber’S Hereditary Optic Neuropathy ◽  
Oxygen Species ◽  
Nitrogen Species ◽  
Leber's Hereditary Optic Neuropathy ◽  
Hereditary Optic Neuropathy

Here we have collected evidence suggesting that chronic changes in the NO homeostasis and the rise of reactive oxygen species bioavailability can contribute to cell dysfunction in Leber’s hereditary optic neuropathy (LHON) patients. We report that peripheral blood mononuclear cells (PBMCs), derived from a female LHON patient with bilateral reduced vision and carrying the pathogenic mutation 11778/ND4, display increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS), as revealed by flow cytometry, fluorometric measurements of nitrite/nitrate, and 3-nitrotyrosine immunodetection. Moreover, viability assays with the tetrazolium dye MTT showed that lymphoblasts from the same patient are more sensitive to prolonged NO exposure, leading to cell death. Taken together these findings suggest that oxidative and nitrosative stress cooperatively play an important role in driving LHON pathology when excess NO remains available over time in the cell environment.

Plasmon-Mediated Generation of Reactive Oxygen Species from Near-Infrared Light Excited Gold Nanocages for Photodynamic Therapyin Vitro

ACS Nano ◽  
2014 ◽  
Vol 8 (7) ◽  
pp. 7260-7271 ◽  
Author(s):  
Liang Gao ◽  
Ru Liu ◽  
Fuping Gao ◽  
Yaling Wang ◽  
Xinglu Jiang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Near Infrared ◽  
Reactive Oxygen ◽  
Infrared Light ◽  
Oxygen Species ◽  
Near Infrared Light ◽  
Gold Nanocages
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