scholarly journals An exploration of nucleic acid liquid biopsy using a glucose meter

2018 ◽  
Vol 9 (14) ◽  
pp. 3517-3522 ◽  
Author(s):  
Yu Gu ◽  
Ting-Ting Zhang ◽  
Zhi-Feng Huang ◽  
Shan-Wen Hu ◽  
Wei Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Liquid Biopsy ◽  
Proof Of Concept ◽  
Circulating Mirna ◽  
Personal Glucose Meter ◽  
Glucose Meter

A proof-of-concept strategy for a circulating miRNA assay using a personal glucose meter (PGM) was proposed.

Digital quantitative detection of serum circulating miRNAs using dual-enhanced magnetobiosensors based on cascaded nucleic acid circuits

2019 ◽  
Vol 55 (91) ◽  
pp. 13733-13736 ◽  
Author(s):  
Chunxue Liu ◽  
Yayun An ◽  
Yuanfu Zhang ◽  
Xia Li ◽  
Qingwang Xue ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Quantitative Detection ◽  
Circulating Mirnas ◽  
Personal Glucose Meter ◽  
Glucose Meter

Here, we developed a dual-enhanced magnetobiosensor based on cascaded nucleic acid circuits for sensitive, portable and digital quantitative detection of circulating miRNAs in serum by a personal glucose meter (PGM).

scholarly journals Giving New Uses to Glucose Meters: Detection of Prostate Cancer

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 24
Author(s):  
Clara Abardía-Serrano ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Prostate Cancer ◽  
Alkaline Phosphatase ◽  
Nucleic Acid ◽  
Cancer Patients ◽  
Magnetic Particles ◽  
Personal Glucose Meter ◽  
Reliable Detection ◽  
Glucose Meter ◽  
Enzyme Alkaline Phosphatase

A sandwich genoassay for the detection of PCA3, a nucleic acid biomarker overexpressed in the urine of prostate cancer patients, has been developed by using the enzyme alkaline phosphatase (ALP) as a tracer of the hybrid generated onto the surface of magnetic particles. ALP converts D-glucose-1-phosphate into D-glucose, which is quantified with a personal glucose meter. The resulting methodology allows the reliable detection of PCA3 at low picomolar levels, thus fostering massive screening of prostate cancer.

scholarly journals New Uses for the Personal Glucose Meter: Detection of Nucleic Acid Biomarkers for Prostate Cancer Screening

Sensors ◽  
2020 ◽  
Vol 20 (19) ◽  
pp. 5514
Author(s):  
Clara Abardía-Serrano ◽  
Rebeca Miranda-Castro ◽  
Noemí de-los-Santos-Álvarez ◽  
María Jesús Lobo-Castañón
Keyword(s):  
Prostate Cancer ◽  
Cancer Screening ◽  
Nucleic Acid ◽  
Magnetic Beads ◽  
Dynamic Range ◽  
Prostate Cancer Screening ◽  
Biological Fluids ◽  
Quantitative Detection ◽  
Personal Glucose Meter ◽  
Glucose Meter

A personal glucose meter (PGM)-based method for quantitative detection of a urinary nucleic acid biomarker in prostate cancer screening, the so-called PCA3, is reported herein. A sandwich-type genoassay is conducted on magnetic beads to collect the target from the sample by specific hybridization, making the assay appropriate for PCA3 detection in biological fluids. The success of the method hinges on the use of alkaline phosphatase (ALP) to link the amount of nucleic acid biomarker to the generation of glucose. In particular, specifically attached ALP molecules hydrolyze D-glucose-1-phosphate into D-glucose, thus enabling the amplification of the recorded signal on the personal glucose meter. The developed genoassay exhibits good sensitivity (3.3 ± 0.2 mg glucose dL−1 pM−1) for PCA3, with a dynamic range of 5 to 100 pM and a quantification limit of 5 pM. Likewise, it facilitates point-of-care testing of nucleic acid biomarkers by using off-the-shelf PGM instead of complex instrumentation involved in traditional laboratory-based tests.

scholarly journals P1DH.8 - An Exploration of Nucleic Acid Liquid Biopsy Using Glucose Meter

2018 ◽  
Author(s):  
J.-J. Xu ◽  
W. Zhao ◽  
Y. Gu ◽  
C.-H. Xu ◽  
H.-Y. Chen
Keyword(s):  
Nucleic Acid ◽  
Liquid Biopsy ◽  
Glucose Meter

scholarly journals Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kanako Saito ◽  
Yuri Ota ◽  
Dieter M. Tourlousse ◽  
Satoko Matsukura ◽  
Hirotsugu Fujitani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Purity ◽  
Droplet Microfluidics ◽  
Individual Growth ◽  
Proof Of Concept ◽  
Widespread Adoption ◽  
Technical Challenges ◽  
On Chip ◽  
Uncultured Microorganisms ◽  
Environmental Microbes

AbstractDroplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.

scholarly journals A glucose meter interface for point-of-care gene circuit-based diagnostics

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Evan Amalfitano ◽  
Margot Karlikow ◽  
Masoud Norouzi ◽  
Katariina Jaenes ◽  
Seray Cicek ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Synthetic Biology ◽  
Molecular Diagnostics ◽  
Point Of Care ◽  
Low Cost ◽  
Gene Circuit ◽  
Reporter Systems ◽  
Glucose Meter ◽  
Pandemic Response ◽  
Glucose Output

AbstractRecent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2.

scholarly journals Longitudinal profiling of circulating miRNA during cardiac allograft rejection: a proof‐of‐concept study

2021 ◽  
Author(s):  
Peter J. Kennel ◽  
Alexandre Yahi ◽  
Yoshifumi Naka ◽  
Donna M. Mancini ◽  
Charles C. Marboe ◽  
...  
Keyword(s):  
Allograft Rejection ◽  
Cardiac Allograft ◽  
Proof Of Concept ◽  
Cardiac Allograft Rejection ◽  
Circulating Mirna ◽  
Concept Study

scholarly journals Correction to “DNAzyme Amplified Aptasensing Platform for Ochratoxin A Detection Using a Personal Glucose Meter”

2021 ◽  
Author(s):  
Songbai Zhang ◽  
Yunxia Luan ◽  
Mengyi Xiong ◽  
Jingjing Zhang ◽  
Ryan Lake ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Personal Glucose Meter ◽  
Glucose Meter

Bioinspired DNA–Inorganic Hybrid Nanoflowers Combined with a Personal Glucose Meter for Onsite Detection of miRNA

2018 ◽  
Vol 10 (49) ◽  
pp. 42050-42057 ◽  
Author(s):  
Tingting Wu ◽  
Yuemeng Yang ◽  
Yu Cao ◽  
Yongchao Song ◽  
Li-Ping Xu ◽  
...  
Keyword(s):  
Inorganic Hybrid ◽  
Personal Glucose Meter ◽  
Glucose Meter
Sign in / Sign up

Export Citation Format

Share Document