scholarly journals Photo-patterned oxygen sensing films based on Pt porphyrin for controlling cell growth and studying metabolism

RSC Advances ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 924-930 ◽  
Author(s):  
Fei Zeng ◽  
Zengju Fan ◽  
Shanshan Wu ◽  
Xing Cheng ◽  
Yanqing Tian
Keyword(s):  
Cell Growth ◽  
Oxygen Sensing ◽  
Oxygen Sensors ◽  
Cell Respiration ◽  
Affect Cell Growth

Developing biocompatible and photo-polymerizable hydrogels with oxygen sensors for microengineering to affect cell growth and monitor cell respiration simultaneously.

scholarly journals Prohibitins Regulate Membrane Protein Degradation by the m-AAA Protease in Mitochondria

1999 ◽  
Vol 19 (5) ◽  
pp. 3435-3442 ◽  
Author(s):  
Gregor Steglich ◽  
Walter Neupert ◽  
Thomas Langer
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Growth ◽  
Membrane Proteins ◽  
Eukaryotic Cells ◽  
Mitochondrial Morphology ◽  
Regulatory Effect ◽  
Inner Membrane ◽  
Functional Activities ◽  
Native Molecular Mass ◽  
Affect Cell Growth

ABSTRACT Prohibitins comprise a protein family in eukaryotic cells with potential roles in senescence and tumor suppression. Phb1p and Phb2p, members of the prohibitin family in Saccharomyces cerevisiae, have been implicated in the regulation of the replicative life span of the cells and in the maintenance of mitochondrial morphology. The functional activities of these proteins, however, have not been elucidated. We demonstrate here that prohibitins regulate the turnover of membrane proteins by the m-AAA protease, a conserved ATP-dependent protease in the inner membrane of mitochondria. The m-AAA protease is composed of the homologous subunits Yta10p (Afg3p) and Yta12p (Rca1p). Deletion ofPHB1 or PHB2 impairs growth of Δyta10 or Δyta12 cells but does not affect cell growth in the presence of the m-AAA protease. A prohibitin complex with a native molecular mass of approximately 2 MDa containing Phb1p and Phb2p forms a supercomplex with them-AAA protease. Proteolysis of nonassembled inner membrane proteins by the m-AAA protease is accelerated in mitochondria lacking Phb1p or Phb2p, indicating a negative regulatory effect of prohibitins on m-AAA protease activity. These results functionally link members of two conserved protein families in eukaryotes to the degradation of membrane proteins in mitochondria.

How Plant Hormones and Their Interactions Affect Cell Growth

2016 ◽  
pp. 174-195 ◽  
Author(s):  
Stephen Depuydt ◽  
Stan Van Praet ◽  
Hilde Nelissen ◽  
Bartel Vanholme ◽  
Danny Vereecke
Keyword(s):  
Cell Growth ◽  
Plant Hormones ◽  
Affect Cell Growth

scholarly journals Flavored e-liquids increase cytoplasmic Ca2+ levels in airway epithelia

2020 ◽  
Vol 318 (2) ◽  
pp. L226-L241 ◽  
Author(s):  
Temperance R. Rowell ◽  
James E. Keating ◽  
Bryan T. Zorn ◽  
Gary L. Glish ◽  
Stephen B. Shears ◽  
...  
Keyword(s):  
Cell Growth ◽  
Biological Effects ◽  
Potential Toxicity ◽  
Airway Epithelia ◽  
Unknown Mechanism ◽  
Kinase C ◽  
Short And Long Term ◽  
Cellular Ca ◽  
Affect Cell Growth

E-cigarettes are noncombustible, electronic nicotine-delivery devices that aerosolize an e-liquid, i.e., nicotine, in a propylene glycol-vegetable glycerin vehicle that also contains flavors. While the effects of nicotine are relatively well understood, more information regarding the potential biological effects of the other e-liquid constituents is needed. This is a serious concern, because e-liquids are available in >7,000 distinct flavors. We previously demonstrated that many e-liquids affect cell growth/viability through an unknown mechanism. Since Ca2+ is a ubiquitous second messenger that regulates cell growth, we characterized the effects of e-liquids on cellular Ca2+ homeostasis. To better understand the extent of this effect, we screened e-liquids for their ability to alter cytosolic Ca2+ levels and found that 42 of 100 flavored e-liquids elicited a cellular Ca2+ response. Banana Pudding (BP) e-liquid, a representative e-liquid from this group, caused phospholipase C activation, endoplasmic reticulum (ER) Ca2+ release, store-operated Ca2+ entry (SOCE), and protein kinase C (PKCα) phosphorylation. However, longer exposures to BP e-liquid depleted ER Ca2+ stores and inhibited SOCE, suggesting that this e-liquid may alter Ca2+ homeostasis by short- and long-term mechanisms. Since dysregulation of Ca2+ signaling can cause chronic inflammation, ER stress, and abnormal cell growth, flavored e-cigarette products that can elicit cell Ca2+ responses should be further screened for potential toxicity.

Microplates with Integrated Oxygen Sensors for Kinetic Cell Respiration Measurement and Cytotoxicity Testing in Primary and Secondary Cell Lines

2005 ◽  
Vol 3 (3) ◽  
pp. 299-307 ◽  
Author(s):  
Rahul Ravi Deshpande ◽  
Yvonne Koch-Kirsch ◽  
Ruth Maas ◽  
Gernot T. John ◽  
Christian Krause ◽  
...  
Keyword(s):  
Cell Lines ◽  
Oxygen Sensors ◽  
Cell Respiration ◽  
Cytotoxicity Testing

scholarly journals Concerning the localization of steroids in centrioles and basal bodies by immunofluorescence.

1978 ◽  
Vol 76 (2) ◽  
pp. 255-260 ◽  
Author(s):  
I Nenci ◽  
E Marchetti
Keyword(s):  
Cell Growth ◽  
Basal Bodies ◽  
Immunofluorescence Technique ◽  
Cell Growth And Differentiation ◽  
Affect Cell Growth

Specific steroid antibodies, by the immunofluorescence technique, regularly reveal fluorescent centrioles and cilia-bearing basal bodies in target and nontarget cells. Although the precise identity of the immunoreactive steroid substance has not yet been established, it seems noteworthy that exogenous steroids can be vitally concentrated by centrioles, perhaps by exchange with steroids already present at this level. This unexpected localization suggests that steroids may affect cell growth and differentiation in some way different from the two-step receptor mechanism.

scholarly journals Robust optical oxygen sensors based on polymer-bound NIR-emitting platinum(ii)–benzoporphyrins

2014 ◽  
Vol 2 (36) ◽  
pp. 7589-7598 ◽  
Author(s):  
L. H. Hutter ◽  
B. J. Müller ◽  
K. Koren ◽  
S. M. Borisov ◽  
I. Klimant
Keyword(s):  
High Performance ◽  
Oxygen Sensing ◽  
Oxygen Sensors ◽  
Covalently Bound

New high performance optical oxygen sensing materials based on NIR-emitting indicators covalently bound to various polymers overcome main limitations of the conventional sensors.

The GntR-type regulators GtrA and GtrB affect cell growth and nodulation of Sinorhizobium meliloti

2008 ◽  
Vol 46 (2) ◽  
pp. 137-145 ◽  
Author(s):  
Yi Wang ◽  
Ai-Min Chen ◽  
Ai-Yuan Yu ◽  
Li Luo ◽  
Guan-Qian Yu ◽  
...  
Keyword(s):  
Cell Growth ◽  
Sinorhizobium Meliloti ◽  
Affect Cell Growth
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