scholarly journals Double-ratiometric fluorescence imaging of H2Se and O2˙− under hypoxia for exploring Na2SeO3-induced HepG2 cells' apoptosis

RSC Advances ◽  
2018 ◽  
Vol 8 (71) ◽  
pp. 40984-40988 ◽  
Author(s):  
Xiaonan Gao ◽  
Wenfei Guo ◽  
Lihong Ge ◽  
Fanpeng Kong ◽  
Kehua Xu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Hepg2 Cells ◽  
Sodium Selenite ◽  
Stress Process ◽  
Ratiometric Fluorescence ◽  
Induced Apoptosis

Sodium selenite (Na2SeO3), as an anti-tumor drug for inducing tumor cells' apoptosis, has been widely studied under normoxic conditions and has been shown to exhibit oxidative stress process-induced apoptosis.

Hybrid Compounds & Oxidative Stress Induced Apoptosis in Cancer Therapy

2020 ◽  
Vol 27 (13) ◽  
pp. 2118-2132 ◽  
Author(s):  
Aysegul Hanikoglu ◽  
Hakan Ozben ◽  
Ferhat Hanikoglu ◽  
Tomris Ozben
Keyword(s):  
Oxidative Stress ◽  
Drug Resistance ◽  
Side Effects ◽  
Cancer Therapy ◽  
Tumor Cells ◽  
Anticancer Drugs ◽  
Chemotherapeutic Agents ◽  
Oxidation Reactions ◽  
Hybrid Compounds ◽  
Induced Apoptosis

: Elevated Reactive Oxygen Species (ROS) generated by the conventional cancer therapies and the endogenous production of ROS have been observed in various types of cancers. In contrast to the harmful effects of oxidative stress in different pathologies other than cancer, ROS can speed anti-tumorigenic signaling and cause apoptosis of tumor cells via oxidative stress as demonstrated in several studies. The primary actions of antioxidants in cells are to provide a redox balance between reduction-oxidation reactions. Antioxidants in tumor cells can scavenge excess ROS, causing resistance to ROS induced apoptosis. Various chemotherapeutic drugs, in their clinical use, have evoked drug resistance and serious side effects. Consequently, drugs having single-targets are not able to provide an effective cancer therapy. Recently, developed hybrid anticancer drugs promise great therapeutic advantages due to their capacity to overcome the limitations encountered with conventional chemotherapeutic agents. Hybrid compounds have advantages in comparison to the single cancer drugs which have usually low solubility, adverse side effects, and drug resistance. This review addresses two important treatments strategies in cancer therapy: oxidative stress induced apoptosis and hybrid anticancer drugs.

scholarly journals Tributylphosphate (TBP) and tris (2-butoxyethyl) phosphate (TBEP) induced apoptosis and cell cycle arrest in HepG2 cells

2017 ◽  
Vol 6 (6) ◽  
pp. 902-911 ◽  
Author(s):  
Guofa Ren ◽  
Jingwen Hu ◽  
Yu Shang ◽  
Yufang Zhong ◽  
Zhiqiang Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Molecular Mechanism ◽  
Hepg2 Cells ◽  
Cytotoxic Effects ◽  
Cycle Arrest ◽  
Induced Apoptosis

The purpose of this study was to investigate the cytotoxic effects of tributylphosphate (TBP) and tris (2-butoxyethyl) phosphate (TBEP) and to explore the underlying molecular mechanism focusing on oxidative stress, apoptosis, and cell cycle arrest.

scholarly journals Optimized models of xenobiotic-induced oxidative stress in HepG2 cells

2021 ◽  
Vol 18 (5) ◽  
pp. 1001-1007
Author(s):  
Yollada Sriset ◽  
Waranya Chatuphonprasert ◽  
Kanokwan Jarukamjorn
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Hepg2 Cells ◽  
Sodium Selenite ◽  
Cellular Injury ◽  
Stress Model ◽  
Total Glutathione ◽  
Tert Butyl Hydroperoxide ◽  
Stress Models

Purpose: To evaluate the molecular impact of ethanol, sodium selenite, and tert-butyl hydroperoxide (TBHP) on oxidant-antioxidant balance in HepG2 cells to establish an optimized oxidative stress model of HepG2 cells. Methods: HepG2 cells were treated with ethanol (10 - 500 mM) and sodium selenite (1 - 10 µM) for 24 and 48 h and with TBHP (50 - 200 µM) for 3 and 24 h, respectively. Biomarkers for cellular injury, ie, lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA), and for antioxidant system, i.e., superoxide dismutase (SOD), catalase (CAT), and total glutathione content, were determined. Results: All treatments increased the levels of LDH, AST, ALT, and MDA but decreased SOD and CAT activities and the total glutathione content in HepG2 cells. Oxidative stress was induced by these oxidative stressors in HepG2 cells via oxidant-antioxidant imbalance, with TBHP (100 µM, 3 h) acting as a powerful oxidant based on the minimal time to induce oxidative stress. The antioxidants, ascorbic acid and gallic acid, improved oxidant-antioxidant imbalance against xenobiotic-induced oxidative stress in HepG2 cells. Conclusion: These oxidative stress models are suitable for investigating the antioxidant and/or hepatoprotective potential of chemicals, including natural compounds.

In Situ Ratiometric Fluorescence Imaging for Tracking Targeted Delivery and Release of Anticancer Drug in Living Tumor Cells

2019 ◽  
Vol 2 (11) ◽  
pp. 4687-4692 ◽  
Author(s):  
Keqin Yang ◽  
Jingjin Zhao ◽  
Liangliang Zhang ◽  
Rongjun Liu ◽  
Hong Liang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescence Imaging ◽  
Anticancer Drug ◽  
Targeted Delivery ◽  
Ratiometric Fluorescence

scholarly journals Ratiometric fluorescence imaging of Golgi H2O2 reveals a correlation between Golgi oxidative stress and hypertension

2019 ◽  
Vol 10 (47) ◽  
pp. 10876-10880 ◽  
Author(s):  
Hui Wang ◽  
Zixu He ◽  
Yuyun Yang ◽  
Jiao Zhang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Living Systems ◽  
Ratiometric Fluorescence ◽  
Two Photon ◽  
Group A ◽  
Ratiometric Imaging

Based on a novel Golgi-targeting phenylsulfonamide group, a two-photon (TP) fluorescent probe, Np-Golgi, was developed for in situ H2O2 ratiometric imaging in living systems.

Real-time tracing the changes in the intracellular pH value during apoptosis by near-infrared ratiometric fluorescence imaging

2018 ◽  
Vol 54 (65) ◽  
pp. 9071-9074 ◽  
Author(s):  
Liyun Lin ◽  
Jingjin Zhao ◽  
Liangliang Zhang ◽  
Yong Huang ◽  
Fanggui Ye ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Intracellular Ph ◽  
Near Infrared ◽  
Ph Value ◽  
Ratiometric Fluorescence ◽  
Dual Emission ◽  
Induced Apoptosis

A single-excitation dual-emission nanoprobe with near-infrared response was developed for real-time tracing the changes in the pH value during hydrogen peroxide-induced apoptosis by ratiometric fluorescence imaging.

scholarly journals A Novel RNA-Binding Protein, Ossa/C9orf10, Regulates Activity of Src Kinases To Protect Cells from Oxidative Stress-Induced Apoptosis

2008 ◽  
Vol 29 (2) ◽  
pp. 402-413 ◽  
Author(s):  
Masamitsu Tanaka ◽  
Kazuki Sasaki ◽  
Reiko Kamata ◽  
Yukari Hoshino ◽  
Kazuyoshi Yanagihara ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Tumor Cells ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Pi3 Kinase ◽  
Scaffolding Protein ◽  
Functional Protein ◽  
Uncharacterized Protein ◽  
Induced Apoptosis

ABSTRACT During the process of tumor progression and clinical treatments, tumor cells are exposed to oxidative stress. Tumor cells are frequently resistant to such stress by producing antiapoptotic signaling, including activation of Src family kinases (SFKs), although the molecular mechanism is not clear. In an attempt to identify the SFK-binding proteins selectively phosphorylated in gastric scirrhous carcinoma, we identified an uncharacterized protein, C9orf10. Here we report that C9orf10 (designated Ossa for oxidative stress-associated Src activator) is a novel RNA-binding protein that guards cancer cells from oxidative stress-induced apoptosis by activation of SFKs. Exposure to oxidative stress such as UV irradiation induces the association of Ossa/C9orf10 with regulatory domains of SFKs, which activates these kinases and causes marked tyrosine phosphorylation of C9orf10 in turn. Tyrosine-phosphorylated Ossa recruits p85 subunits of phosphatidylinositol 3-kinase (PI3-kinase) and behaves as a scaffolding protein for PI3-kinase and SFKs, which activates the Akt-mediated antiapoptotic pathway. On the other hand, the carboxyl terminus of Ossa has a distinct function that directly binds RNAs such as insulin-like growth factor II (IGF-II) mRNA and promotes the extracellular secretion of IGF-II. Our findings indicate that Ossa is a dual-functional protein and might be a novel therapeutic target which modulates the sensitivity of tumors to oxidative stress.

NF-κB, JNK and p53 pathways are involved in tubeimoside-1-induced apoptosis in HepG2 cells with oxidative stress and G2/M cell cycle arrest

2011 ◽  
Vol 49 (12) ◽  
pp. 3046-3054 ◽  
Author(s):  
Yan Yin ◽  
Wei Chen ◽  
Changyan Tang ◽  
Hanjing Ding ◽  
Jongchol Jang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Hepg2 Cells ◽  
M Cell ◽  
Cycle Arrest ◽  
Induced Apoptosis
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