scholarly journals A metabolic interpretation for the response of cellular autofluorescence to chemical perturbations assessed using spectral phasor analysis

RSC Advances ◽  
2018 ◽  
Vol 8 (72) ◽  
pp. 41526-41535
Author(s):  
Jeff Maltas ◽  
Dylan Palo ◽  
Chong Kai Wong ◽  
Symeon Stefan ◽  
James O'Connor ◽  
...  
Keyword(s):  
Two Component ◽  
Phasor Analysis ◽  
Chemical Perturbations

The two-component nature of the autofluorescence response is indicative of whether a single or several pathways are affected.

scholarly journals Cellular NADH and NADPH Conformation as a Real-Time Fluorescence-Based Metabolic Indicator under Pressurized Conditions

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 5020
Author(s):  
Martin Heidelman ◽  
Bibek Dhakal ◽  
Millicent Gikunda ◽  
Kalinga Pavan Thushara Silva ◽  
Laxmi Risal ◽  
...  
Keyword(s):  
Real Time ◽  
Metabolic Response ◽  
Ambient Pressure ◽  
Spectral Behavior ◽  
Metabolic Monitoring ◽  
Respiratory Inhibitor ◽  
Two Component ◽  
Autofluorescence Spectroscopy ◽  
Agriculture And Food ◽  
Phasor Analysis

Cellular conformation of reduced pyridine nucleotides NADH and NADPH sensed using autofluorescence spectroscopy is presented as a real-time metabolic indicator under pressurized conditions. The approach provides information on the role of pressure in energy metabolism and antioxidant defense with applications in agriculture and food technologies. Here, we use spectral phasor analysis on UV-excited autofluorescence from Saccharomyces cerevisiae (baker’s yeast) to assess the involvement of one or multiple NADH- or NADPH-linked pathways based on the presence of two-component spectral behavior during a metabolic response. To demonstrate metabolic monitoring under pressure, we first present the autofluorescence response to cyanide (a respiratory inhibitor) at 32 MPa. Although ambient and high-pressure responses remain similar, pressure itself also induces a response that is consistent with a change in cellular redox state and ROS production. Next, as an example of an autofluorescence response altered by pressurization, we investigate the response to ethanol at ambient, 12 MPa, and 30 MPa pressure. Ethanol (another respiratory inhibitor) and cyanide induce similar responses at ambient pressure. The onset of non-two-component spectral behavior upon pressurization suggests a change in the mechanism of ethanol action. Overall, results point to new avenues of investigation in piezophysiology by providing a way of visualizing metabolism and mitochondrial function under pressurized conditions.

Spectroscopic Observations of Visual Double Stars

1965 ◽  
Vol 5 ◽  
pp. 109-111
Author(s):  
Frederick R. West
Keyword(s):  
Radial Velocity ◽  
High Dispersion ◽  
Velocity Difference ◽  
Spectrograph Slit ◽  
Spectroscopic Observations ◽  
Double Stars ◽  
Visual Double Stars ◽  
Relative Orbit ◽  
Two Component ◽  
Star Images

There are certain visual double stars which, when close to a node of their relative orbit, should have enough radial velocity difference (10-20 km/s) that the spectra of the two component stars will appear resolved on high-dispersion spectrograms (5 Å/mm or less) obtainable by use of modern coudé and solar spectrographs on bright stars. Both star images are then recorded simultaneously on the spectrograph slit, so that two stellar components will appear on each spectrogram.

A technique for protecting delicate specimens during processing

1989 ◽  
Vol 47 ◽  
pp. 982-983
Author(s):  
R.J. Mount ◽  
R.V. Harrison
Keyword(s):  
Basilar Membrane ◽  
Low Cost ◽  
Organ Of Corti ◽  
Region Of Interest ◽  
Sensory Epithelium ◽  
Physical Damage ◽  
Air Drying ◽  
Specimen Handling ◽  
Two Component

The sensory end organ of the ear, the organ of Corti, rests on a thin basilar membrane which lies between the bone of the central modiolus and the bony wall of the cochlea. In vivo, the organ of Corti is protected by the bony wall which totally surrounds it. In order to examine the sensory epithelium by scanning electron microscopy it is necessary to dissect away the protective bone and expose the region of interest (Fig. 1). This leaves the fragile organ of Corti susceptible to physical damage during subsequent handling. In our laboratory cochlear specimens, after dissection, are routinely prepared by the O-T- O-T-O technique, critical point dried and then lightly sputter coated with gold. This processing involves considerable specimen handling including several hours on a rotator during which the organ of Corti is at risk of being physically damaged. The following procedure uses low cost, readily available materials to hold the specimen during processing ,preventing physical damage while allowing an unhindered exchange of fluids.Following fixation, the cochlea is dehydrated to 70% ethanol then dissected under ethanol to prevent air drying. The holder is prepared by punching a hole in the flexible snap cap of a Wheaton vial with a paper hole punch. A small amount of two component epoxy putty is well mixed then pushed through the hole in the cap. The putty on the inner cap is formed into a “cup” to hold the specimen (Fig. 2), the putty on the outside is smoothed into a “button” to give good attachment even when the cap is flexed during handling (Fig. 3). The cap is submerged in the 70% ethanol, the bone at the base of the cochlea is seated into the cup and the sides of the cup squeezed with forceps to grip it (Fig.4). Several types of epoxy putty have been tried, most are either soluble in ethanol to some degree or do not set in ethanol. The only putty we find successful is “DUROtm MASTERMENDtm Epoxy Extra Strength Ribbon” (Loctite Corp., Cleveland, Ohio), this is a blue and yellow ribbon which is kneaded to form a green putty, it is available at many hardware stores.

Fifth virial coefficient of a two-component mixture of hard discs

1997 ◽  
Vol 90 (4) ◽  
pp. 679-681
Author(s):  
F. SAIJA ◽  
G. FIUMARA ◽  
P.V. GIAQUINTA
Keyword(s):  
Virial Coefficient ◽  
Component Mixture ◽  
Two Component

Phase transitions and shapes of two component membranes and vesicles II : weak segregation limit

1994 ◽  
Vol 4 (8) ◽  
pp. 1333-1362 ◽  
Author(s):  
Takashi Taniguchi ◽  
Kyozi Kawasaki ◽  
David Andelman ◽  
Toshihiro Kawakatsu
Keyword(s):  
Phase Transitions ◽  
Two Component

Strongly coupled nonneutral plasmas : Equilibrium and relaxation in two-component plasmas in Penning-Malmberg trap

2000 ◽  
Vol 10 (PR5) ◽  
pp. Pr5-271-Pr5-274
Author(s):  
H. Totsuji ◽  
K. Tsuruta ◽  
C. Totsuji ◽  
K. Nakano ◽  
T. Kishimoto ◽  
...  
Keyword(s):  
Strongly Coupled ◽  
Nonneutral Plasmas ◽  
Two Component

Features of products for processing of two-component mixes of wheat and flax

2018 ◽  
Vol 0 (12) ◽  
pp. 42-46
Author(s):  
G.N. Pankratov ◽  
◽  
E.P. Meleshkina ◽  
I.V. Vitol ◽  
R.Kh. Kandrokov ◽  
...  
Keyword(s):  
Two Component
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