scholarly journals Chemical studies on the parasitic plant Thonningia sanguinea Vahl

RSC Advances ◽  
2018 ◽  
Vol 8 (37) ◽  
pp. 21002-21011 ◽  
Author(s):  
Ama Kyeraa Thomford ◽  
Reda Fouad Ahmed Abdelhameed ◽  
Koji Yamada
Keyword(s):  
Parasitic Plant ◽  
Plant Sterols ◽  
Significant Evidence ◽  
Chemical Studies ◽  
Thonningia Sanguinea ◽  
First Time

A detailed first-time report on plant sterols and one flavanone characterized from Thonningia sanguinea provides significant evidence for its ethnomedicinal usage.

scholarly journals Cyclam-Based Chelators Bearing Phosphonated Pyridine Pendants for 64Cu-PET Imaging : Synthesis, Physico-Chemical Studies, Radiolabeling and Bioimaging.

2020 ◽  
Author(s):  
Richard Knighton ◽  
Thibault Troadec ◽  
Valerie Mazan ◽  
Patricia La Saëc ◽  
Séverine Marionneau-Lambot ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Copper Complexes ◽  
The Body ◽  
Renal Elimination ◽  
X Ray Diffraction ◽  
Physico Chemical ◽  
Chemical Studies ◽  
First Time ◽  
Excellent Stability

Herein we present the preparation of two novel cyclam-based macrocycles (te1pyp and cb-te1pyp), bearing phosphonate-appended pyridine side-arms for the coordination of copper(II) ions in the context of 64Cu PET imaging. The two ligands have been prepared through conventional protection-alkylation sequences on cyclam, and their coordination properties have been thoroughly investigated. The corresponding copper complexes have been fully characterized in the solid-state (X-Ray diffraction analysis) and in solution (EPR and UV-Vis spectroscopies). Potentiometric studies, combined with spectrometry, have also allowed us to determine their thermodynamic stability constants, confirming their high affinity for copper(II) cations. The kinetic inertness of the complexes has also been verified by acid-assisted dissociation experiments, enabling their use in 64Cu-PET imaging in mice for the first time. Indeed, the two ligands could be quantitatively radiolabeled under mild conditions, and the resulting 64Cu complexes have demonstrated excellent stability in serum. PET imaging demon-strated a set of features emerging from the combination of picolinates and phosphonate units: high stability in vivo, fast clear-ance from the body via renal elimination, and most interestingly, very low fixation in the liver. The latter is in contrast with what was observed for monopicolinate cyclam (te1pa), that had a non-negligible accumulation in the liver, owing probably to its different charge and lipophillicity. These results thus pave the way for the use of such phosphonated pyridine chelators for in vivo 64Cu-PET imaging.

scholarly journals Cyclam-Based Chelators Bearing Phosphonated Pyridine Pendants for 64Cu-PET Imaging : Synthesis, Physico-Chemical Studies, Radiolabeling and Bioimaging.

2020 ◽  
Author(s):  
Richard Knighton ◽  
Thibault Troadec ◽  
Valerie Mazan ◽  
Patricia La Saëc ◽  
Séverine Marionneau-Lambot ◽  
...  
Keyword(s):  
Pet Imaging ◽  
Copper Complexes ◽  
The Body ◽  
Renal Elimination ◽  
X Ray Diffraction ◽  
Physico Chemical ◽  
Chemical Studies ◽  
First Time ◽  
Excellent Stability

Herein we present the preparation of two novel cyclam-based macrocycles (te1pyp and cb-te1pyp), bearing phosphonate-appended pyridine side-arms for the coordination of copper(II) ions in the context of 64Cu PET imaging. The two ligands have been prepared through conventional protection-alkylation sequences on cyclam, and their coordination properties have been thoroughly investigated. The corresponding copper complexes have been fully characterized in the solid-state (X-Ray diffraction analysis) and in solution (EPR and UV-Vis spectroscopies). Potentiometric studies, combined with spectrometry, have also allowed us to determine their thermodynamic stability constants, confirming their high affinity for copper(II) cations. The kinetic inertness of the complexes has also been verified by acid-assisted dissociation experiments, enabling their use in 64Cu-PET imaging in mice for the first time. Indeed, the two ligands could be quantitatively radiolabeled under mild conditions, and the resulting 64Cu complexes have demonstrated excellent stability in serum. PET imaging demon-strated a set of features emerging from the combination of picolinates and phosphonate units: high stability in vivo, fast clear-ance from the body via renal elimination, and most interestingly, very low fixation in the liver. The latter is in contrast with what was observed for monopicolinate cyclam (te1pa), that had a non-negligible accumulation in the liver, owing probably to its different charge and lipophillicity. These results thus pave the way for the use of such phosphonated pyridine chelators for in vivo 64Cu-PET imaging.

scholarly journals Coffee carbohydrates

2006 ◽  
Vol 18 (1) ◽  
pp. 165-174 ◽  
Author(s):  
Robert Redgwell ◽  
Monica Fischer
Keyword(s):  
Cell Wall ◽  
Potential Role ◽  
Structural Features ◽  
Coffee Bean ◽  
Cell Wall Polysaccharides ◽  
Pectic Polysaccharides ◽  
Specific Polysaccharide ◽  
Chemical Studies ◽  
First Time ◽  
Detailed Chemical

This review summarises recent advances in the chemistry, physiology and molecular properties of coffee carbohydrates with a particular focus on the cell wall polysaccharides. The results of detailed chemical studies have demonstrated novel structural features of both the galactomannans and the arabinogalactan polysaccharides of the green and roasted coffee bean. For the first time immunological probes based on monoclonal antibodies for specific polysaccharide epitopes were used to reveal the patterns of distribution of the galactomannans, arabinogalactans and pectic polysaccharides in the coffee bean cell wall. Finally, the results of physiological and molecular studies are presented which emphasise the growing awareness of the potential role the metabolic status of the green bean may play in final coffee beverage quality.

scholarly journals De novo transcriptome sequencing of Rhododendron molle and identification of genes involved in the biosynthesis of secondary metabolites

2020 ◽  
Author(s):  
Guolin Zhou ◽  
Ping Zhu
Keyword(s):  
Secondary Metabolites ◽  
Transcriptome Sequencing ◽  
De Novo ◽  
Illumina Hiseq ◽  
Medicinal Value ◽  
Relieve Pain ◽  
Chemical Studies ◽  
Illumina Hiseq Platform ◽  
Differential Gene ◽  
First Time

Abstract Background: Rhododendron molle (Ericaceae) is a traditional Chinese medicinal plant, its flower and root have been widely used to treat rheumatism and relieve pain for thousands of years in China. Chemical studies have revealed that R. molle contains abundant secondary metabolites such as terpenoinds, flavonoids and lignans, some of which have exhibited various bioactivities including antioxidant, hypotension and analgesic activity. In spite of immense pharmaceutical importance, the mechanism underlying the biosynthesis of secondary metabolites remains unknown and the genomic information is unavailable. Results: To gain molecular insight into this plant, especially on the information of pharmaceutically important secondary metabolites including grayanane diterpenoids, we conducted deep transcriptome sequencing for R. molle flower and root using the Illumina Hiseq platform. In total, 100,603 unigenes were generated through de novo assembly with mean length of 778 bp, 57.1% of these unigenes were annotated in public databases and 17,906 of those unigenes showed significant match in the KEGG database. Unigenes involved in the biosynthesis of secondary metabolites were annotated, including the TPSs and CYPs that were potentially responsible for the biosynthesis of grayanoids. Moreover, 3,376 transcription factors and 10,828 simple sequence repeats (SSRs) were also identified. Additionally, we further performed differential gene expression (DEG) analysis of the flower and root transcriptome libraries and identified numerous genes that were specifically expressed or up-regulated in flower.Conclusions: To the best of our knowledge, this is the first time to generate and thoroughly analyze the transcriptome data of both R. molle flower and root. This study provided an important genetic resource which will shed light on elucidating various secondary metabolite biosynthetic pathways in R. molle, especially for those with medicinal value and allow for drug development in this plant.

scholarly journals De novo transcriptome sequencing of Rhododendron molle and identification of genes involved in the biosynthesis of secondary metabolites

2020 ◽  
Author(s):  
Guolin Zhou ◽  
Ping Zhu
Keyword(s):  
Secondary Metabolites ◽  
Transcriptome Sequencing ◽  
De Novo ◽  
Illumina Hiseq ◽  
Medicinal Value ◽  
Relieve Pain ◽  
Chemical Studies ◽  
Illumina Hiseq Platform ◽  
Differential Gene ◽  
First Time

Abstract Background: Rhododendron molle (Ericaceae) is a traditional Chinese medicinal plant, its flower and root have been widely used to treat rheumatism and relieve pain for thousands of years in China. Chemical studies have revealed that R. molle contains abundant secondary metabolites such as terpenoinds, flavonoids and lignans, some of which have exhibited various bioactivities including antioxidant, hypotension and analgesic activity. In spite of immense pharmaceutical importance, the mechanism underlying the biosynthesis of secondary metabolites remains unknown and the genomic information is unavailable. Results: To gain molecular insight into this plant, especially on the pharmaceutically important secondary metabolic information, we conducted deep transcriptome sequencing for R. molle flower and root using the Illumina Hiseq platform. In total, 100,603 unigenes were generated through de novo assembly with mean length of 778 bp, 57.1% of these unigenes were annotated in public databases and 17,906 of those unigenes showed significant match in the KEGG database. Unigenes involved in the biosynthesis of secondary metabolites were annotated, including the TPSs and CYPs that were potentially responsible for the biosynthesis of grayanoids. Moreover, 3,376 transcription factors and 10,828 simple sequence repeats (SSRs) were also identified. Additionally, we further performed differential gene expression (DEG) analysis of the flower and root transcriptome libraries and identified numerous genes that were specifically expressed or up-regulated in flower.Conclusions: To the best of our knowledge, this is the first time to generate and thoroughly analyze the transcriptome data of both R. molle flower and root. This study provided an important genetic resource which will shed light on elucidating various secondary metabolite biosynthetic pathways in R. molle, especially for those with medicinal value and allow for drug development in this plant.

scholarly journals Unveiling a surprising diversity in the lichen genus Micarea (Pilocarpaceae) in Réunion (Mascarenes archipelago, Indian Ocean)

2014 ◽  
Vol 46 (3) ◽  
pp. 413-439 ◽  
Author(s):  
A. Maarten BRAND ◽  
Pieter P. G. VAN DEN BOOM ◽  
Emmanuël SÉRUSIAUX
Keyword(s):  
Indian Ocean ◽  
Tropical Island ◽  
The Status ◽  
Chemical Studies ◽  
The Indian Ocean ◽  
Suitable Habitats ◽  
Almost All ◽  
Local Ecology ◽  
First Time ◽  
Made In

AbstractDetailed anatomical and chemical studies conducted on recent collections made in almost all suitable habitats on Réunion, a small remote tropical island in the Indian Ocean, yielded a surprising diversity in the widespread lichen genus Micarea (Pilocarpaceae, Lecanorales). Twenty-one species are recognized, including 13 described here as new to science. They are: Micarea alectorialica, M. bebourensis, M. borbonica, M. boryana, M. cilaoensis, M. hyalinoxanthonica, M. isidiosa, M. melanoprasina, M. pseudocoppinsii, M. pseudolignaria, M. sublithinella, M. takamakae and M. tenuispora. Notes on local ecology and important biogeographical features are also given and a key to the species is provided. Isidiiform areolae are reported for the first time in the genus (M. isidiosa and M. tenuispora), as well as the production of protolichesterinic and confluentic acids (M. sublithinella and M. takamakae, respectively). Two groups within the genus are species-rich on the island: the M. peliocarpa group with possibly 5 species, including 3 new to science, and the M. prasina group with 4 species, including 2 new to science. Micarea levicula is reported here for the first time since its description, and the status of the material that can be referred to M. micrococca s. lat. needs further study.

scholarly journals De novo transcriptome sequencing of Rhododendron molle and identification of genes involved in the biosynthesis of secondary metabolites

2019 ◽  
Author(s):  
Guolin Zhou ◽  
Ping Zhu
Keyword(s):  
Secondary Metabolites ◽  
Transcriptome Sequencing ◽  
De Novo ◽  
Illumina Hiseq ◽  
Medicinal Value ◽  
Relieve Pain ◽  
Chemical Studies ◽  
Illumina Hiseq Platform ◽  
Differential Gene ◽  
First Time

Abstract Background Rhododendron molle (Ericaceae) is a traditional Chinese medicinal plant, its flower and root have been widely used to treat rheumatism and relieve pain for thousands of years in China. Chemical studies have revealed that R. molle contains abundant secondary metabolites such as terpenoinds, flavonoids and lignans, some of which have exhibited various bioactivities including analgesia and hypotension. In despite of immense pharmaceutical importance, the mechanism underlying the biosynthesis of secondary metabolites remains unknown and the genomic information is unavailable.Results To gain molecular insight into the R. molle , we conducted deep transcriptome sequencing for R. molle flower and root using the Illumina Hiseq platform. In total, 100,603 unigenes were generated through de novo assembly with mean length of 778 bp, 57.1% of these unigenes were annotated in public databases and 17,906 of those unigenes showed significant match in the KEGG database. Unigenes involved in biosynthesis of secondary metabolites were annotated. Moreover, 3,376 transcription factors and 10,828 simple sequence repeats (SSRs) were also identified. Additionally, we further performed differential gene expression (DEG) analysis of the flower and root transcriptome libraries and identified numerous genes that were specifically expressed or up-regulated in flower.Conclusions To the best of our knowledge, this is the first time to generate and thoroughly analyze the transcriptome data of both R. molle flower and root. This study provided an important genetic resource which will shed light on elucidating various secondary metabolite biosynthetic pathways in R. molle , especially for those with medicinal value and allow for drug development in this herb.

scholarly journals De novo transcriptome sequencing of Rhododendron molle and identification of genes involved in the biosynthesis of secondary metabolites

2020 ◽  
Author(s):  
Guolin Zhou ◽  
Ping Zhu
Keyword(s):  
Secondary Metabolites ◽  
Transcriptome Sequencing ◽  
De Novo ◽  
Illumina Hiseq ◽  
Medicinal Value ◽  
Relieve Pain ◽  
Chemical Studies ◽  
Illumina Hiseq Platform ◽  
Differential Gene ◽  
First Time

Abstract Background: Rhododendron molle (Ericaceae) is a traditional Chinese medicinal plant, its flower and root have been widely used to treat rheumatism and relieve pain for thousands of years in China. Chemical studies have revealed that R. molle contains abundant secondary metabolites such as terpenoinds, flavonoids and lignans, some of which have exhibited various bioactivities including antioxidant, hypotension and analgesic activity. In spite of immense pharmaceutical importance, the mechanism underlying the biosynthesis of secondary metabolites remains unknown and the genomic information is unavailable. Results: To gain molecular insight into this plant, especially on the information of pharmaceutically important secondary metabolites including grayanane diterpenoids, we conducted deep transcriptome sequencing for R. molle flower and root using the Illumina Hiseq platform. In total, 100,603 unigenes were generated through de novo assembly with mean length of 778 bp, 57.1% of these unigenes were annotated in public databases and 17,906 of those unigenes showed significant match in the KEGG database. Unigenes involved in the biosynthesis of secondary metabolites were annotated, including the TPSs and CYPs that were potentially responsible for the biosynthesis of grayanoids. Moreover, 3,376 transcription factors and 10,828 simple sequence repeats (SSRs) were also identified. Additionally, we further performed differential gene expression (DEG) analysis of the flower and root transcriptome libraries and identified numerous genes that were specifically expressed or up-regulated in flower.Conclusions: To the best of our knowledge, this is the first time to generate and thoroughly analyze the transcriptome data of both R. molle flower and root. This study provided an important genetic resource which will shed light on elucidating various secondary metabolite biosynthetic pathways in R. molle, especially for those with medicinal value and allow for drug development in this plant.

Enzymes Inhibitory Constituents From Buddleja Crispa

2005 ◽  
Vol 60 (3) ◽  
pp. 341-346 ◽  
Author(s):  
Ijaz Ahmad ◽  
Abdul Malik ◽  
Nighat Afza ◽  
Itrat Anis ◽  
Itrat Fatima ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Methyl Benzoate ◽  
Spectroscopic Techniques ◽  
Chemical Studies ◽  
First Time

Steroidal galactoside 1 and aryl esters 2 and 3 have been isolated from Buddleja crispa, along with ginipin 4, gardiol 5, 1-heptacosanol 6, and methyl benzoate 7, isolated for the first time from this species. The structures of all of the compounds were determined by spectroscopic techniques and chemical studies. The steroidal galactoside 1 is an inhibitor of lipoxygenase. Compounds 1- 3 displayed inhibitory activity against butyrylcholinesterse, while compounds 2 and 3 further showed inhibition against acetylcholinesterase

scholarly journals A first report of the Broad-Tail Royal Creon cleobis Godart, 1824 (Insecta: Lepidoptera: Lycaenidae) and its host plant from Agasthyamalai Biosphere Reserve of the southern Western Ghats, India

2017 ◽  
Vol 9 (5) ◽  
pp. 10240
Author(s):  
Raveendran Lekshmi
Keyword(s):  
Literature Review ◽  
Host Plant ◽  
Western Ghats ◽  
Biosphere Reserve ◽  
Parasitic Plant ◽  
Field Observations ◽  
Wildlife Sanctuary ◽  
Southern Western Ghats ◽  
Lycaenid Butterfly ◽  
First Time

The lycaenid butterfly Broad-tail Royal Creon cleobis is being reported for the first time from Agasthyamalai Biosphere Reserve, at Peppara Wildlife Sanctuary.  Adults were observed within the elevations of 1,000–1,350 m in November 2014.  The eggs, larva and pupa were found on Helixanthera intermedia, an endemic parasitic plant found in the southern Western Ghats.  Based on field observations and literature review, the peak occurrence of this butterfly seems to be during the months September–November.

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