scholarly journals Dual-spectra encoded suspension array using reversed-phase microemulsion UV curing and electrostatic self-assembling

RSC Advances ◽  
2018 ◽  
Vol 8 (38) ◽  
pp. 21272-21279 ◽  
Author(s):  
Guangxia Feng ◽  
Qinghua He ◽  
WenYue Xie ◽  
Yonghong He ◽  
Xuejing Chen ◽  
...  
Keyword(s):  
High Throughput ◽  
Rapid Growth ◽  
Uv Curing ◽  
Reversed Phase ◽  
Suspension Array ◽  
Self Assembling ◽  
Modern Biotechnology

The rapid growth of demand for high-throughput multiplexed biochips from modern biotechnology has led to growing interest in suspension array based on multi-channel encoded microbeads.

scholarly journals Combination of Suspension Array and Mycelial Growth Assay for Detecting Multiple-Fungicide Resistance in Botrytis cinerea in Hebei Province in China

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1213-1219
Author(s):  
Zehua Su ◽  
Xin Zhang ◽  
Jianjiang Zhao ◽  
Wenqiao Wang ◽  
Lei Shang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
High Throughput ◽  
Fungicide Resistance ◽  
Mycelial Growth ◽  
Accurate Method ◽  
Hebei Province ◽  
Combination Method ◽  
Major Type ◽  
Suspension Array ◽  
Growth Assay

To provide a high-throughput, efficient, and accurate method to monitor multiple-fungicide resistance of Botrytis cinerea in the field, we used the suspension array, sequencing, and mycelial growth assay in our research. Discriminating-dose bioassays for detecting carbendazim, diethofencarb, boscalid, and iprodione resistance (CarR, DieR, BosR, and IprR, respectively) were used to analyze 257 isolates collected from Hebei Province in China during 2016 and 2017. High resistance frequencies to carbendazim (100%), diethofencarb (92.08%), and iprodione (86.59%) were detected. BosR isolates accounted for 11.67% of the total. In addition, 103 isolates were randomly selected for phenotype and genotype detection. The high-throughput suspension array was utilized to detect eight genotypes simultaneously, including BenA-E198, BenA-198A, SdhB-H272, SdhB-272Y, BcOS1-I365, BcOS1-365S, erg27-F412, and erg27-412S, which were associated with resistance toward carbendazim or diethofencarb, boscalid, iprodione, and fenhexamid (FenR), respectively. Most of the benzimidazole-resistant isolates (81.55%) possessed the E198V mutation in the BenA gene. Ninety-three isolates with dual resistance to carbendazim and diethofencarb showed the E198V/K mutation. All BosR isolates carried the H272R mutation in the SdhB gene. The I365S and Q369P+N373S (66.99%) mutations in the BcOS1 gene were more frequently observed. No mutation was detected in the erg27 gene in Hebei isolates. There were 13 resistance profile phenotypes. Phenotypes with triple resistance were the most common (83.50%), and CarRDieRBosSIprRFenS was the major type. CarR isolates that carried E198V/K/A were all highly resistant (HR) and only one F200Y mutant was moderately resistant (MR) to carbendazim. Isolates that possessed E198V/K were MR or HR to diethofencarb. BosR isolates that possessed H272R mutation were lowly resistant (LR). IprR isolates were all LR or MR. The distribution of half maximal effective concentrations of CarR isolates with E198V/K mutations and IprR isolates with Q369P+N373S mutations significantly increased from 2016 to 2017. Combined with our observations, a combination method of the high-throughput suspension array and the mycelial growth assay was suggested to accurately monitor multiple resistance of B. cinerea in the field.

Simultaneous detection of five antibiotics in milk by high-throughput suspension array technology

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1160-1165 ◽  
Author(s):  
Pu Su ◽  
Nan Liu ◽  
Maoxiang Zhu ◽  
Baoan Ning ◽  
Ming Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Simultaneous Detection ◽  
Suspension Array ◽  
Array Technology

Self-assembling of multi-jets by pyro-electrohydrodynamic effect for high throughput liquid nanodrops transfer

Lab on a Chip ◽  
2011 ◽  
Vol 11 (19) ◽  
pp. 3294 ◽  
Author(s):  
Sara Coppola ◽  
Veronica Vespini ◽  
Simonetta Grilli ◽  
Pietro Ferraro
Keyword(s):  
High Throughput ◽  
Self Assembling

High-throughput simultaneous genotyping of human platelet antigen-1 to -16 by using suspension array

Transfusion ◽  
2013 ◽  
Vol 53 (11) ◽  
pp. 2722-2728 ◽  
Author(s):  
Qun-Xing An ◽  
Cui-Ying Li ◽  
Li-Juan Xu ◽  
Xian-Qing Zhang ◽  
Yan-Jun Bai ◽  
...  
Keyword(s):  
High Throughput ◽  
Human Platelet ◽  
Suspension Array ◽  
Platelet Antigen ◽  
Human Platelet Antigen

Cytometric Microbead Magnetic Suspension Array for High-Throughput Ultrasensitive Detection of Aflatoxin B1

2018 ◽  
Vol 91 (1) ◽  
pp. 1194-1202 ◽  
Author(s):  
Xiaofei Liu ◽  
Guangyao Ying ◽  
Xiaofang Liao ◽  
Chaonan Sun ◽  
Fang Wei ◽  
...  
Keyword(s):  
High Throughput ◽  
Aflatoxin B1 ◽  
Magnetic Suspension ◽  
Ultrasensitive Detection ◽  
Suspension Array

scholarly journals A high-throughput and ultrasensitive identification methodology for unauthorized GMP component based on suspension array and logical calculator

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Pengyu Zhu ◽  
Wei Fu ◽  
Shuang Wei ◽  
Xiao Liu ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Suspension Array

scholarly journals High-throughput morphology mapping of self-assembling ternary polymer blends

RSC Advances ◽  
2020 ◽  
Vol 10 (69) ◽  
pp. 42529-42541
Author(s):  
Kristof Toth ◽  
Chinedum O. Osuji ◽  
Kevin G. Yager ◽  
Gregory S. Doerk
Keyword(s):  
Block Copolymer ◽  
High Throughput ◽  
Grazing Incidence ◽  
Ternary Mixtures ◽  
Electrospray Deposition ◽  
X Ray ◽  
Copolymer Blends ◽  
Self Assembling ◽  
X Ray Scattering ◽  
Ternary Polymer

Compositionally graded electrospray deposition combined with grazing incidence small angle X-ray scattering forms a high-throughput approach for mapping phase behavior in ternary mixtures as demonstrated here using block copolymer blends.

scholarly journals Optimisation and standardisation of a multiplex immunoassay of diverse Plasmodium falciparum antigens to assess changes in malaria transmission using sero-epidemiology

2019 ◽  
Vol 4 ◽  
pp. 26 ◽  
Author(s):  
Lindsey Wu ◽  
Tom Hall ◽  
Isaac Ssewanyana ◽  
Tate Oulton ◽  
Catriona Patterson ◽  
...  
Keyword(s):  
Quality Control ◽  
Plasmodium Falciparum ◽  
Serum Sample ◽  
High Throughput ◽  
High Sensitivity ◽  
Positive Control ◽  
Antibody Responses ◽  
Suspension Array ◽  
Epidemiological Surveys ◽  
Positive Controls

Background: Antibody responses have been used to characterise transmission and exposure history in malaria-endemic settings for over a decade. Such studies have typically been conducted on well-standardised enzyme-linked immunosorbent assays (ELISAs). However, recently developed quantitative suspension array technologies (qSAT) are now capable of high-throughput and multiplexed screening of up to hundreds of analytes at a time. This study presents a customised protocol for the Luminex MAGPIX© qSAT using a diverse set of malaria antigens. The aim is to develop a standardised assay for routine serological surveillance that is implementable across laboratories and epidemiological settings. Methods: A panel of eight Plasmodium falciparum recombinant antigens, associated with long- and short-lived antibody responses, was designed for the Luminex MAGPIX© platform. The assay was optimised for key steps in the protocol: antigen-bead coupling concentration, buffer composition, serum sample dilution, and bead storage conditions. Quality control procedures and data normalisation methods were developed to address high-throughput assay processing.  Antigen-specific limits of quantification (LOQs) were also estimated using both in-house and WHO reference serum as positive controls. Results: Antigen-specific bead coupling was optimised across five serum dilutions and two positive controls, resulting in concentrations operational within stable analytical ranges. Coupled beads were stable after storage at room temperature (22⁰C) for up to eight weeks. High sensitivity and specificity for distinguishing positive and negative controls at serum sample dilutions of 1:500 (AUC 0.94 95%CI 0.91-0.96) and 1:1000 (AUC 0.96 95%CI 0.94-0.98) were observed. LOQs were also successfully estimated for all analytes but varied by antigen and positive control. Conclusions: This study demonstrates that developing a standardised malaria-specific qSAT protocol for a diverse set of antigens is achievable, though further optimisations may be required. Quality control and data standardisation methods may also be useful for future analysis of large sero-epidemiological surveys.

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