scholarly journals A ratiometric fluorescence assay for acetylcholinesterase activity and inhibitor screening based on supramolecular assembly induced monomer–excimer emission transition of a perylene probe

RSC Advances ◽  
2018 ◽  
Vol 8 (23) ◽  
pp. 12785-12790 ◽  
Author(s):  
Chunhua He ◽  
Huipeng Zhou ◽  
Ejaz Hussain ◽  
Yunyi Zhang ◽  
Niu Niu ◽  
...  
Keyword(s):  
Lauric Acid ◽  
Ache Activity ◽  
Fluorescence Probe ◽  
Supramolecular Assembly ◽  
Acetylcholinesterase Activity ◽  
Supramolecular Assemblies ◽  
Ratiometric Fluorescence ◽  
Excimer Emission ◽  
Inhibitor Screening ◽  
Emission Transition

The PDI-DHA can aggregate to form supramolecular assemblies when mixed with lauroylcholine and lauric acid, and is employed as a ratiometric fluorescence probe for the detection of AChE activity.

Phenylsulfonic acid functionalized carbon quantum dots based biosensor for acetylcholinesterase activity monitoring and inhibitor screening

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105454-105460 ◽  
Author(s):  
Fengqi Zhou ◽  
Hui Feng ◽  
Yafen Fang ◽  
Qian Sun ◽  
Zhaosheng Qian
Keyword(s):  
Quantum Dots ◽  
Ache Activity ◽  
Acetylcholinesterase Activity ◽  
Carbon Quantum Dots ◽  
Activity Monitoring ◽  
Inhibitor Screening

Phenylsulfonic acid functionalized carbon quantum dots (PSA-CQDs) were prepared and used to construct a convenient and reliable fluorometric biosensor for acetylcholinesterase (AChE) activity and inhibitor screening.

A sensitive ratiometric fluorescence probe for chymotrypsin activity and inhibitor screening

2018 ◽  
Vol 273 ◽  
pp. 204-210 ◽  
Author(s):  
Yiping Chen ◽  
Jing Cao ◽  
Xiaoxue Jiang ◽  
Zhizhen Pan ◽  
Nanyan Fu
Keyword(s):  
Fluorescence Probe ◽  
Ratiometric Fluorescence ◽  
Inhibitor Screening ◽  
Chymotrypsin Activity

A Ratiometric Fluorescence Probe for Selective Visual Sensing of Zn2+

2005 ◽  
Vol 127 (43) ◽  
pp. 14962-14963 ◽  
Author(s):  
Ayyappanpillai Ajayaghosh ◽  
Priya Carol ◽  
Sivaramapanicker Sreejith
Keyword(s):  
Fluorescence Probe ◽  
Ratiometric Fluorescence ◽  
Visual Sensing

Ratiometric fluorescence probe for two-photon bioimaging of Cr3+ in living cells

2014 ◽  
Vol 55 (30) ◽  
pp. 4075-4077 ◽  
Author(s):  
Zhiguo Zhou ◽  
Ying Li ◽  
Yongquan Wu
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Ratiometric Fluorescence ◽  
Two Photon

scholarly journals The Toxic Effect of Manganese on the Acetylcholinesterase Activity in Rat Brains

2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Vahid Yousefi Babadi ◽  
Leila Sadeghi ◽  
Kobra Shirani ◽  
Ali Akbar Malekirad ◽  
Mohammad Rezaei
Keyword(s):  
Toxic Effect ◽  
Ache Activity ◽  
Time Course ◽  
Acetylcholinesterase Activity ◽  
Time Intervals ◽  
Chronic Dose ◽  
Essential Nutrient ◽  
Short Time ◽  
Chronic Doses ◽  
Dose Dependent

Manganese (Mn) is a naturally occurring element and an essential nutrient for humans and animals. However, exposure to high levels of Mn may cause neurotoxic effects. Accumulation of manganese damages central nervous system and causes Parkinson’s disease-like syndrome called manganism. Mn neurotoxicity has been suggested to involve an imbalance between the DAergic and cholinergic systems. The pathological mechanisms associated with Mn neurotoxicity are poorly understood, but several reports have established it is mediated by changing of AChE activity that resulted in oxidative stress. Therefore we focused the effect of Mn in AChE activity in the rat’s brain by MnCl2injection intraperitoneally and analyzed their brains after time intervals. This study used different acute doses in short time course and different chronic doses at different exposing time to investigate which of them (exposing dose or time) is more important in Mn toxic effect. Results showed toxic effect of Mn is highly dose dependent and AChE activity in presence of chronic dose in 8 weeks reaches acute dose in only 2 days.

Evaluation of acetylcholinesterase activity and behavioural alterations induced by ketamine in an animal model of schizophrenia

2013 ◽  
Vol 26 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Alexandra I. Zugno ◽  
Maria Paula Matos ◽  
Leila Canever ◽  
Daiane B. Fraga ◽  
Renata D. De Luca ◽  
...  
Keyword(s):  
Animal Model ◽  
Cognitive Deficits ◽  
Cholinergic System ◽  
Ache Activity ◽  
Cognitive Deficit ◽  
Chronic Administration ◽  
Inhibitory Avoidance ◽  
Acetylcholinesterase Activity ◽  
Chronic Effects ◽  
Behavioural Tests

ObjectiveCognitive deficits in schizophrenia play a crucial role in its clinical manifestation and seem to be related to changes in the cholinergic system, specifically the action of acetylcholinesterase (AChE). Considering this context, the aim of this study was to evaluate the chronic effects of ketamine in the activity of AChE, as well as in behavioural parameters involving learning and memory.MethodsThe ketamine was administered for 7 days. A duration of 24 h after the last injection, the animals were submitted to behavioural tests. The activity of AChE in prefrontal cortex, hippocampus and striatum was measured at different times after the last injection (1, 3, 6 and 24 h).ResultsThe results indicate that ketamine did not affect locomotor activity and stereotypical movements. However, a cognitive deficit was observed in these animals by examining their behaviour in inhibitory avoidance. In addition, an increase in AChE activity was observed in all structures analysed 1, 3 and 6 h after the last injection. Differently, serum activity of AChE was similar between groups.ConclusionChronic administration of ketamine in an animal model of schizophrenia generates increased AChE levels in different brain tissues of rats that lead to cognitive deficits. Therefore, further studies are needed to elucidate the complex mechanisms associated with schizophrenia.

Determination of L-ascorbic Acid by Carbon Quantum Dot/Eosin Y Ratiometric Fluorescence Probe

2020 ◽  
Vol 41 (3) ◽  
pp. 331-338
Author(s):  
武文波 WU Wen-bo ◽  
张越诚 ZHANG Yue-cheng ◽  
李承佳 LI Cheng-jia ◽  
马红燕 MA Hong-yan ◽  
杨晓军 YANG Xiao-jun
Keyword(s):  
Ascorbic Acid ◽  
Quantum Dot ◽  
Fluorescence Probe ◽  
Eosin Y ◽  
Ratiometric Fluorescence ◽  
Carbon Quantum Dot
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