2-Aminoimidazole facilitates efficient gene delivery in a low molecular weight poly(amidoamine) dendrimer

2018 ◽  
Vol 16 (24) ◽  
pp. 4464-4470 ◽  
Author(s):  
Jing Wang ◽  
Xuefeng Hu ◽  
Dongli Wang ◽  
Cao Xie ◽  
Weiyue Lu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Low Molecular Weight ◽  
Efficient Gene

2-Aminoimidazole greatly improved the transfection efficiency of G2. It contributes to condensing DNA into small, monodisperse nanostructures, enhancing cellular penetration and endosome/lysosome escape.

Zinc ion coordination significantly improved the transfection efficiency of low molecular weight polyethylenimine

2019 ◽  
Vol 7 (4) ◽  
pp. 1716-1728 ◽  
Author(s):  
Huapan Fang ◽  
Lin Lin ◽  
Jie Chen ◽  
Jiayan Wu ◽  
Huayu Tian ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Zinc Ion ◽  
Low Molecular Weight ◽  
Gene Delivery System ◽  
System P

A zinc ion coordination-contained polycationic gene delivery system.

Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles

2015 ◽  
Vol 3 (13) ◽  
pp. 2660-2670 ◽  
Author(s):  
Qian Guo ◽  
Yan-Hong Liu ◽  
Miao-Miao Xun ◽  
Ji Zhang ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Glycidyl Ether ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

PEI 600-based polymers were synthesized via ring-opening polymerization and exhibited much better transfection efficiency and biocompatibility than PEI 25 kDa.

Starburst low-molecular weight polyethylenimine for efficient gene delivery

2011 ◽  
Vol 100A (1) ◽  
pp. 134-140 ◽  
Author(s):  
Yanjun Zhao ◽  
Rulei Yang ◽  
Dong Liu ◽  
Mingjing Sun ◽  
Lijun Zhou ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
Efficient Gene

scholarly journals Amino Acid-Linked Low Molecular Weight Polyethylenimine for Improved Gene Delivery and Biocompatibility

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 975 ◽  
Author(s):  
Xiao-Ru Wu ◽  
Ji Zhang ◽  
Ju-Hui Zhang ◽  
Ya-Ping Xiao ◽  
Xi He ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gel Permeation Chromatography ◽  
Viral Gene ◽  
Low Molecular Weight ◽  
Ph Buffering ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

The construction of efficient and low toxic non-viral gene delivery vectors is of great significance for gene therapy. Herein, two novel polycations were constructed via Michael addition from low molecular weight polyethylenimine (PEI) 600 Da and amino acid-containing linkages. Lysine and histidine were introduced for the purpose of improved DNA binding and pH buffering capacity, respectively. The ester bonds afforded the polymer biodegradability, which was confirmed by the gel permeation chromatography (GPC) measurement. The polymers could well condense DNA into nanoparticles and protect DNA from degradation by nuclease. Compared with PEI 25 kDa, these polymers showed higher transfection efficiency, lower toxicity, and better serum tolerance. Study of this mechanism revealed that the polyplexes enter the cells mainly through caveolae-mediated endocytosis pathway; this, together with their biodegradability, facilitates the internalization of polyplexes and the release of DNA. The results reveal that the amino acid-linked low molecular weight PEI polymers could serve as promising candidates for non-viral gene delivery.

scholarly journals A Low-Molecular-Weight Polyethylenimine/pDNA-VEGF Polyplex System Constructed in a One-Pot Manner for Hindlimb Ischemia Therapy

Pharmaceutics ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 171 ◽  
Author(s):  
Xiaoshuang Guo ◽  
Zihan Yuan ◽  
Yang Xu ◽  
Xiaotian Zhao ◽  
Zhiwei Fang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Low Molecular Weight ◽  
Physical Interaction ◽  
Hindlimb Ischemia ◽  
One Pot

Peripheral arterial disease (PAD) is often characterized by continued reduction in blood flow supply to limbs. Advanced therapeutic strategies like gene therapy could potentially be applied to limb ischemia therapy. However, developing a gene delivery system with low toxicity and high efficiency remains a great challenge. In this study, a one-pot construction was used to integrate vector synthesis and polyplex fabrication simultaneously in a simple and robust manner. We fabricated an interpenetrating gene delivery network through the physical interaction between low-molecular-weight polyethylenimine (PEI 1.8 kDa) and plasmid DNA (pDNA) and the chemical bonding between PEI and glutaraldehyde (GA), which was named the glutaraldehydelinked-branched PEI (GPEI) polyplex. The final GPEI polyplex system was pH-responsive and biodegradable due to the imine linkage and it could successfully deliver desired vascular endothelial growth factor (VEGF) pDNA. Compared with PEI (25 kDa)/pDNA polyplexes, GPEI polyplexes showed lower cytotoxicity and higher transfection efficiency both in vitro and in vivo. In addition, we demonstrated that GPEI polyplexes could efficiently promote the formation of new capillaries in vivo, which may provide a practicable strategy for clinical hindlimb ischemia therapy in the future.

Low molecular weight PEI-based fluorinated polymers for efficient gene delivery

2019 ◽  
Vol 162 ◽  
pp. 602-611 ◽  
Author(s):  
Ya-Ping Xiao ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
Ju-Hui Zhang ◽  
Qing-Ying Yu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
Fluorinated Polymers ◽  
Efficient Gene

Gene delivery with low molecular weight linear polyethylenimines

2005 ◽  
Vol 7 (10) ◽  
pp. 1287-1298 ◽  
Author(s):  
Miriam Breunig ◽  
Uta Lungwitz ◽  
Renate Liebl ◽  
Claudia Fontanari ◽  
Juergen Klar ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight
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