Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles

2015 ◽  
Vol 3 (13) ◽  
pp. 2660-2670 ◽  
Author(s):  
Qian Guo ◽  
Yan-Hong Liu ◽  
Miao-Miao Xun ◽  
Ji Zhang ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Glycidyl Ether ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

PEI 600-based polymers were synthesized via ring-opening polymerization and exhibited much better transfection efficiency and biocompatibility than PEI 25 kDa.

Bio-reducible polycations from ring-opening polymerization as potential gene delivery vehicles

2016 ◽  
Vol 14 (27) ◽  
pp. 6470-6478 ◽  
Author(s):  
Qing-Ying Yu ◽  
Yan-Hong Liu ◽  
Zheng Huang ◽  
Ji Zhang ◽  
Chao-Ran Luan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Ring Opening Polymerization ◽  
Dna Condensation ◽  
Molecular Weights ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

Bio-reducible polycations were prepared via ring-opening polymerization. These materials have relatively low molecular weights and cytotoxicity but have good DNA condensation ability, transfection efficiency and excellent serum tolerance.

Zinc ion coordination significantly improved the transfection efficiency of low molecular weight polyethylenimine

2019 ◽  
Vol 7 (4) ◽  
pp. 1716-1728 ◽  
Author(s):  
Huapan Fang ◽  
Lin Lin ◽  
Jie Chen ◽  
Jiayan Wu ◽  
Huayu Tian ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Delivery System ◽  
Transfection Efficiency ◽  
Zinc Ion ◽  
Low Molecular Weight ◽  
Gene Delivery System ◽  
System P

A zinc ion coordination-contained polycationic gene delivery system.

Amphiphilic polymers formed from ring-opening polymerization: a strategy for the enhancement of gene delivery

2017 ◽  
Vol 5 (4) ◽  
pp. 718-729 ◽  
Author(s):  
Yi-Mei Zhang ◽  
Zheng Huang ◽  
Ji Zhang ◽  
Wan-Xia Wu ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Ring Opening Polymerization ◽  
Amphiphilic Polymers ◽  
Cationic Lipids ◽  
Promising Strategy

Ring-opening polymerization was found to be a promising strategy to improve the transfection efficiency and serum tolerance of cationic lipids.

scholarly journals Pentamethylcyclopentadienyl Molybdenum(V) Complexes Derived from Iodoanilines: Synthesis, Structure, and ROP of ε-Caprolactone

Catalysts ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1554
Author(s):  
Tian Xing ◽  
Mark R. J. Elsegood ◽  
Sophie H. Dale ◽  
Carl Redshaw
Keyword(s):  
Molecular Weight ◽  
Comparative Studies ◽  
Ring Opening ◽  
Kinetic Studies ◽  
Ring Opening Polymerization ◽  
Molecular Structures ◽  
Low Molecular Weight ◽  
End Groups ◽  
Amine Complex ◽  
Reaction Stoichiometry

The reaction of [Mo(η-C5Me5)Cl4] with the ortho-, meta-, or para-iodo-functionalized anilines 2-IC6H4NH2, 3-IC6H4NH2, 4-IC6H4NH2 yields imido or amine products of the type [Mo(η-C5Me5)Cl2(IC6H4N)] (2-I, 1, 3-I, 3, 4-I, 5) or [Mo(η-C5Me5)Cl4(IC6H4NH2)] (3-I, 2, 4-I, 4), respectively, depending on the reaction stoichiometry/conditions; we were unable to isolate an amine complex of the 2-I derivative. The reaction of [Mo(η-C5Me5)Cl4] with one equivalent of 2-I,4-FC6H3NH2 in the presence of Et3N afforded [Mo(η-C5Me5)Cl2(2-I,4-FC6H3N)]·MeCN (6·MeCN), which, upon exposure to air, afforded the Mo(VI) imido complex [Mo(η-C5Me5)Cl3(2-I,4-FC6H3N)] (7). For comparative studies, the structure of the aniline (C6H5NH2)-derived complex [Mo(η-C5Me5)Cl2(2-C6H3N)] (8) has also been prepared. The molecular structures of 1–8 have been determined and reveal packing in the form of zig-zag chains or ladders. The complexes catalyze, in the presence of benzyl alcohol under N2, the ring-opening polymerization (ROP) of ε-caprolactone affording relatively low molecular weight products. The MALDI-ToF spectra indicate that a number of polymer series bearing a variety of end groups are formed. Conducting the ROPs as melts or under air results in the isolation of higher molecular weight products, again bearing a variety of end groups. Kinetic studies reveal the aniline-derived imido complex 8 performs best, whilst a meta-iodo substituent and a Mo(V) centre are also found to be beneficial. The structures of the side products 2-IC6H4NH3Cl and 3-IC6H4NH3Cl are also reported.

scholarly journals Amino Acid-Linked Low Molecular Weight Polyethylenimine for Improved Gene Delivery and Biocompatibility

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 975 ◽  
Author(s):  
Xiao-Ru Wu ◽  
Ji Zhang ◽  
Ju-Hui Zhang ◽  
Ya-Ping Xiao ◽  
Xi He ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Amino Acid ◽  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gel Permeation Chromatography ◽  
Viral Gene ◽  
Low Molecular Weight ◽  
Ph Buffering ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

The construction of efficient and low toxic non-viral gene delivery vectors is of great significance for gene therapy. Herein, two novel polycations were constructed via Michael addition from low molecular weight polyethylenimine (PEI) 600 Da and amino acid-containing linkages. Lysine and histidine were introduced for the purpose of improved DNA binding and pH buffering capacity, respectively. The ester bonds afforded the polymer biodegradability, which was confirmed by the gel permeation chromatography (GPC) measurement. The polymers could well condense DNA into nanoparticles and protect DNA from degradation by nuclease. Compared with PEI 25 kDa, these polymers showed higher transfection efficiency, lower toxicity, and better serum tolerance. Study of this mechanism revealed that the polyplexes enter the cells mainly through caveolae-mediated endocytosis pathway; this, together with their biodegradability, facilitates the internalization of polyplexes and the release of DNA. The results reveal that the amino acid-linked low molecular weight PEI polymers could serve as promising candidates for non-viral gene delivery.

scholarly journals A Low-Molecular-Weight Polyethylenimine/pDNA-VEGF Polyplex System Constructed in a One-Pot Manner for Hindlimb Ischemia Therapy

Pharmaceutics ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 171 ◽  
Author(s):  
Xiaoshuang Guo ◽  
Zihan Yuan ◽  
Yang Xu ◽  
Xiaotian Zhao ◽  
Zhiwei Fang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Low Molecular Weight ◽  
Physical Interaction ◽  
Hindlimb Ischemia ◽  
One Pot

Peripheral arterial disease (PAD) is often characterized by continued reduction in blood flow supply to limbs. Advanced therapeutic strategies like gene therapy could potentially be applied to limb ischemia therapy. However, developing a gene delivery system with low toxicity and high efficiency remains a great challenge. In this study, a one-pot construction was used to integrate vector synthesis and polyplex fabrication simultaneously in a simple and robust manner. We fabricated an interpenetrating gene delivery network through the physical interaction between low-molecular-weight polyethylenimine (PEI 1.8 kDa) and plasmid DNA (pDNA) and the chemical bonding between PEI and glutaraldehyde (GA), which was named the glutaraldehydelinked-branched PEI (GPEI) polyplex. The final GPEI polyplex system was pH-responsive and biodegradable due to the imine linkage and it could successfully deliver desired vascular endothelial growth factor (VEGF) pDNA. Compared with PEI (25 kDa)/pDNA polyplexes, GPEI polyplexes showed lower cytotoxicity and higher transfection efficiency both in vitro and in vivo. In addition, we demonstrated that GPEI polyplexes could efficiently promote the formation of new capillaries in vivo, which may provide a practicable strategy for clinical hindlimb ischemia therapy in the future.

scholarly journals Fast and Convenient Synthesis of Amine-Terminated Polylactide as a Macroinitiator forω-Benzyloxycarbonyl-L-Lysine-N-Carboxyanhydrides

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Mingjie Ju ◽  
Feirong Gong ◽  
Shujun Cheng ◽  
Yun Gao
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Ring Opening ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Amine Group ◽  
H Nmr ◽  
Convenient Synthesis ◽  
Ft Ir ◽  
Chain Lengths

Amine-terminated poly (L-lactide) (NH2-PLLA) with various chain lengths were successfully synthesized by sequential tert-butyl-N-(3-hydroxypropyl) carbamate initiated bulk ring-opening polymerization (ROP) of L-lactide (L-LA) in the presence of Stannous(II) 2-ethylhexanoate (Sn(Oct)2) and deprotection of theN-tert-butoxycarbonyl (Boc) group at the end of the polymer chain. The polymers obtained were characterized by FT-IR,1H NMR, and GPC method. NH2-PLLA thus prepared was used to initiate the polymerization of ω-benzyloxycarbonyl-L-lysine-N-carboxyanhydride (Lys (Z)-NCA), and the result confirmed the high nucleophilicity of the terminal amine group. This method was not only suitable for the preparation of low molecular weight NH2-PLLA, but also quite efficient in the synthesis of high molecular weight samples.

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