scholarly journals IGF signalling and endocytosis in the human villous placenta in early pregnancy as revealed by comparing quantum dot conjugates with a soluble ligand

Nanoscale ◽  
2019 ◽  
Vol 11 (25) ◽  
pp. 12285-12295 ◽  
Author(s):  
Magdalena Karolczak-Bayatti ◽  
Karen Forbes ◽  
James Horn ◽  
Tambet Teesalu ◽  
Lynda K. Harris ◽  
...  
Keyword(s):  
Growth Factors ◽  
Quantum Dot ◽  
Early Pregnancy ◽  
Complex Combination ◽  
Soluble Ligand ◽  
Cell Layers

A complex combination of trafficking and signaling occurs at the surface of the placenta. We explain how signals from maternal growth factors may be transmitted to deeper cell layers.

Profiling Chemokines, Cytokines and Growth Factors in Human Early Pregnancy Decidua By Protein Array

2007 ◽  
Vol 58 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Sabine Engert ◽  
Lorenz Rieger ◽  
Michaela Kapp ◽  
Jürgen C. Becker ◽  
Johannes Dietl ◽  
...  
Keyword(s):  
Growth Factors ◽  
Early Pregnancy ◽  
Protein Array

scholarly journals Early Pregnancy Human Decidua Gamma/Delta T Cells Exhibit the Tissue Resident and Specific Functional characteristics

2021 ◽  
Author(s):  
Shuo Yang ◽  
Ting Feng ◽  
Yong Cheng Ma ◽  
Hao Tie Wang ◽  
Qin Hong Chen ◽  
...  
Keyword(s):  
T Cells ◽  
Growth Factors ◽  
Fetal Growth ◽  
Early Pregnancy ◽  
Mononuclear Cells ◽  
A549 Cells ◽  
Γδ T Cells ◽  
Normal Pregnancy ◽  
Peripheral Blood Mononuclear ◽  
Research Attention

Abstract Background: A successful pregnancy is a complicated process that builds upon two aspects of the maternal immune system that needs to be balanced. As one of the dominant groups of cells at the maternal fetal interface, the decidual γδ T cells have attracted great research attention in normal pregnancy or miscarriage. However, the role of γδ T cells in fetal growth still remains poorly studied. Results: In this study, we identified γδ T cells were enriched and resident in decidua during early pregnancy, and early decidual γδ T cells were involved in the secretion of growth factors, including growth differentiation factor 15 (GDF15) and bone morphogenetic protein 1 (BMP1). Decrease of these growth factors could impaire fetal development, resulting in fetal growth restriction. We also observed that the early decidual γδ T cells exhibited stronger cytokine secretion characteristic, but its cytotoxicity against A549 cells was weaker, when compared with the γδ T cells in peripheral blood mononuclear cells (PBMCs). In addition, the functional abilities of early decidual γδ T cells in promoting trophoblast cell proliferation, migration, invasion and tube formation were also significantly stronger than those in γδ T cells of PBMCs. Conclusions: These findings highlighted the importance of γδ T cells in fetal growth and maternal immune tolerance during pregnancy, which is different from γδ T cells in PBMCs, and encouraged further research in this field.

scholarly journals Suppressor of cytokine signalling (SOCS) genes are expressed in the endometrium and regulated by conceptus signals during early pregnancy in the ewe

2005 ◽  
Vol 34 (3) ◽  
pp. 637-644 ◽  
Author(s):  
Olivier Sandra ◽  
Isabelle Bataillon ◽  
Pascale Roux ◽  
Jacques Martal ◽  
Gilles Charpigny ◽  
...  
Keyword(s):  
Growth Factors ◽  
Early Pregnancy ◽  
Negative Regulation ◽  
Signalling Pathway ◽  
Cytokine Signaling ◽  
Suppressor Of Cytokine Signaling ◽  
Stimulation Of

It is established that the conceptus–endometrium dialogue involves cytokines, growth factors and hormones. Given the crucial functions of the suppressor of cytokine signaling (SOCS) family proteins in cytokine signalling, we analyzed the expression and the regulation of CIS and SOCSs 1–3 transcripts during early pregnancy in the ovine endometrium. An overall stimulation of the SOCS transcripts was described in the pregnant ewes with two specific patterns. Unilaterally pregnant ewes confirmed the conceptus-produced factors as regulators of the SOCSs 1–3 expression at day 16 of pregnancy. Intrauterine injection of recombinant ovine interferon τ (IFNτ) in cyclic ewes stimulated the expression of the SOCS mRNA with various potencies, therefore suggesting that the SOCS could take part in the negative regulation of the IFNτ signalling pathway.

scholarly journals Porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 gene expression

Reproduction ◽  
2006 ◽  
Vol 132 (6) ◽  
pp. 939-947 ◽  
Author(s):  
S C Fernando ◽  
J S Buck ◽  
M D Ashworth ◽  
J W Ross ◽  
R D Geisert ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factors ◽  
Mrna Expression ◽  
Estrous Cycle ◽  
Early Pregnancy ◽  
Tissue Remodeling ◽  
Uterine Epithelium ◽  
Tissue Kallikrein ◽  
Rt Pcr

Previous studies have suggested that the porcine endometrium may express several tissue kallikreins during the estrous cycle and early pregnancy. The present study investigated porcine endometrial and conceptus tissue kallikrein 1, 4, 11, and 14 mRNA expression during the estrous cycle and early pregnancy. Tissue kallikrein (KLK) gene expression was evaluated using quantitative RT-PCR and in situ hybridization. KLK1 expression was similar across the estrous cycle and early pregnancy, and localized to the endometrial luminal (L) and glandular (G) epithelium. KLK4 endometrial mRNA expression was greatest on days 0, 5, and 10 when compared with days 12, 15, and 17 of the estrous cycle and greater in cyclic compared with pregnant gilts. Expression of KLK4 was more intense in the stroma and uterine epithelium from days 0 to 10 of the estrous cycle. Endometrial KLK11 mRNA was not different between cyclic and pregnant gilts but the expression was greatest on days 10 and 12 compared with all other days evaluated. There was an increased intensity of KLK11 gene expression in the stratum compactum on day 10 of the estrous cycle and early pregnancy. Endometrial KLK14 mRNA expression was not detectable on days 5 and 10 but was expressed on days 0, 12, 15, and 17 of the estrous cycle and pregnancy. KLK14 expression was localized in the uterine L and G epithelium, and stroma throughout the endometrium after day 10. Conceptus KLK1 mRNA did not change from days 10 to 17 of gestation. However, conceptus KLK4, and 14 mRNA expression was greatest on day 10 with expression declining after day 14 of gestation. Expression of the various tissue kallikreins in the endometrium and conceptus during the estrous cycle and early pregnancy in the pig can serve in the activation of growth factors and tissue remodeling during the establishment of pregnancy.

Actin crosslinking protein filamin A during early pregnancy in the rat uterus

2016 ◽  
Vol 28 (7) ◽  
pp. 960 ◽  
Author(s):  
Romanthi J. Madawala ◽  
Connie E. Poon ◽  
Samson N. Dowland ◽  
Christopher R. Murphy
Keyword(s):  
Actin Cytoskeleton ◽  
Early Pregnancy ◽  
Filamin A ◽  
Uterine Receptivity ◽  
Rat Uterus ◽  
Decidual Cells ◽  
Cell Layers ◽  
Important Regulator ◽  
Blastocyst Implantation ◽  
Major Transformation

During early pregnancy the endometrium undergoes a major transformation in order for it to become receptive to blastocyst implantation. The actin cytoskeleton and plasma membrane of luminal uterine epithelial cells (UECs) and the underlying stromal cells undergo dramatic remodelling to facilitate these changes. Filamin A (FLNA), a protein that crosslinks actin filaments and also mediates the anchorage of membrane proteins to the actin cytoskeleton, was investigated in the rat uterus at fertilisation (Day 1) and implantation (Day 6) to determine the role of FLNA in actin cytoskeletal remodelling of UECs and decidua during early pregnancy. Localisation of FLNA in UECs at the time of fertilisation was cytoplasmic, whilst at implantation it was distributed apically; its localisation is under the influence of progesterone. FLNA was also concentrated to the first two to three stromal cell layers at the time of fertilisation and shifted to the primary decidualisation zone at the time of implantation. This shift in localisation was found to be dependent on the decidualisation reaction. Protein abundance of the FLNA 280-kDa monomer and calpain-cleaved fragment (240 kDa) did not change during early pregnancy in UECs. Since major actin cytoskeletal remodelling occurs during early pregnancy in UECs and in decidual cells, the changing localisation of FLNA suggests that it may be an important regulator of cytoskeletal remodelling of these cells to allow uterine receptivity and decidualisation necessary for implantation in the rat.

Growth factors in the biology of human trophoblast and their clinical relevances in early pregnancy maintenance

Placenta ◽  
1993 ◽  
Vol 14 ◽  
pp. 251-263
Author(s):  
Takeshi Maruo ◽  
Hiroya Matsuo ◽  
Kazuaki Katayama ◽  
Cecilia A. Ladines-Llave ◽  
Augusto S. Manalo ◽  
...  
Keyword(s):  
Growth Factors ◽  
Early Pregnancy ◽  
Human Trophoblast

Cellular Proliferation and Fibroblast Growth Factors in the Corpus Luteum during Early Pregnancy in Ewes

1997 ◽  
Vol 14 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Albina Jablonka-Shariff ◽  
Anna T. Grazul-Bilska ◽  
Dale A. Redmer ◽  
Lawrence P. Reynolds
Keyword(s):  
Growth Factors ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Cellular Proliferation ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth
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