High-specificity double-stranded DNA detection with a “humanoid” molecular beacon and TALEs

Nanoscale ◽  
2018 ◽  
Vol 10 (38) ◽  
pp. 18354-18361 ◽  
Author(s):  
Yu Wang ◽  
Jialei Bai ◽  
Xiaochen Qu ◽  
Yifei Gao ◽  
Jiang Wang ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Dna Detection ◽  
High Specificity ◽  
Fluorescence Signal ◽  
Specific Recognition ◽  
Double Stranded Dna ◽  
Signal Output

Ultra-sensitive and high-specificity detection of dsDNA based on TALEs-specific recognition of DNA properties and H-MB fluorescence signal output capability.

Electrochemical molecular beacon biosensor for sequence-specific recognition of double-stranded DNA

2014 ◽  
Vol 59 ◽  
pp. 54-57 ◽  
Author(s):  
Xiangmin Miao ◽  
Xiaoting Guo ◽  
Zhiyou Xiao ◽  
Liansheng Ling
Keyword(s):  
Molecular Beacon ◽  
Specific Recognition ◽  
Double Stranded Dna

A colorimetric method for the sequence-specific recognition of double-stranded DNA on the surface of a silver-coated glass slide

2018 ◽  
Vol 96 (5) ◽  
pp. 466-470
Author(s):  
Xiaoting Guo ◽  
Jing Wang ◽  
Zhifang Zhu ◽  
Manjun Zhang ◽  
Haigang Li ◽  
...  
Keyword(s):  
Colorimetric Method ◽  
Colour Change ◽  
Glass Slide ◽  
Coated Glass ◽  
Specific Recognition ◽  
Double Stranded Dna ◽  
Pcr Products ◽  
Polymerase Chain ◽  
Good Sequence ◽  
Coated Glass Slide

In this study, a colorimetric method for sequence-specific recognition of double-stranded DNA (dsDNA) was established on the surface of a silver-coated glass slide. Oligo-1 was assembled on the surface of a silver-coated glass slide through an Ag–S bond, and Oligo-2 as reporter was used to bind with streptavidin-horseradish peroxidase (SA–HRP). They could bind with target dsDNA that was composed of Oligo-3 and Oligo-4 on the surface of a silver-coated glass slide through triplex formation. The bound HRP could be moved into the solution by DNase I and catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, the concentration of target dsDNA could be determined with the colour change of TMB. Under the optimum conditions, the absorbance was proportional to the concentration of target dsDNA over the range of 100 pmol/L to 2.0 nmol/L, with a detection limit of 13 pmol/L. In addition, this method showed good sequence selectivity, enabling it to be further developed for the detection of other polymerase chain reaction (PCR) products.

scholarly journals A one-step and label-free, electrochemical DNA detection using metal ion-mediated molecular beacon probe

2019 ◽  
Vol 100 ◽  
pp. 64-69 ◽  
Author(s):  
Songyi Baek ◽  
Jun Ki Ahn ◽  
Byoung Yeon Won ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Metal Ion ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Label Free ◽  
One Step ◽  
Electrochemical Dna Detection

A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (6) ◽  
pp. 4019-4025 ◽  
Author(s):  
Fulin Zhou ◽  
Baoxin Li ◽  
Jiyuan Ma
Keyword(s):  
Fluorescence Quenching ◽  
Molecular Beacon ◽  
Dna Detection ◽  
Rolling Circle Amplification ◽  
Dna Probes ◽  
Dna Probe ◽  
Rolling Circle ◽  
Hairpin Probe ◽  
Linear Dna ◽  
Signal Probe

Linear single-labeled DNA probes are used in this RCA-based fluorescence strategy for DNA detection, which could effectively avoid the fluorescence quenching between neighboring signal probes using hairpin probe as signal probe.

A graphene-enhanced molecular beacon for homogeneous DNA detection

Nanoscale ◽  
2010 ◽  
Vol 2 (6) ◽  
pp. 1021 ◽  
Author(s):  
Fan Li ◽  
Yan Huang ◽  
Qing Yang ◽  
Zentao Zhong ◽  
Di Li ◽  
...  
Keyword(s):  
Molecular Beacon ◽  
Dna Detection
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